GAO Feng-mei,SHAO Li-gang,WANG Yan,et al.SSR Molecular Characterization and Applied Research ofWheat Scab Major Resistance Genes in Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(11):15-20.
春小麦赤霉病主效抗性基因的SSR分子检测与应用研究
- Title:
- SSR Molecular Characterization and Applied Research ofWheat Scab Major Resistance Genes in Spring Wheat
- 文章编号:
- 1002-2767（2012）11-0015-06
- 分类号:
- S512.034
- 文献标志码:
- A
- 摘要:
- 为提高黑龙江省农业科学院小麦赤霉病抗性种质创新能力和抗赤育种水平，向生产提供优良的小麦赤霉病抗性新材料,以苏麦3号衍生材料为研究对象，以病小穗为评价指标，运用单位花滴注对2011年产量鉴定13份材料、F618份材料、F532份材料进行田间抗赤霉病性鉴定，同时用覆盖苏麦3号、在3BS、6BS上与小麦抗赤霉病紧密连锁的主效抗性基因相关的SSR标记：Xgwm493、Xwmg533、Xgwm389、barc133、barc147、barc102（3BS）和Xgwm644、Xgwm133、barc101（6BS）对这些材料进行分子检测，以研究其表现型与基因型的关系。结果表明：经田间鉴定出的25份抗性材料中15份抗性材料在主效抗性位点3BS和6BS上分别存在相应的抗性基因。说明这15份材料具有表现型和基因型的统一。有10份抗性材料在3BS和6BS上均不存在相应抗性基因，20份感病材料3BS和6BS上存在相应的抗性基因。
- Abstract:
- The Sumai No.3 derived materials were used as the research materials,the disease-spikelet was regarded as evaluation index,the unit flower drip-method was applied to evaluate 13 materials of production evaluation nursery,18 materials of F6 generation and 32 materials of F5 generation from 2011 year for field scab resistance evaluation,and using the cover Sumai No.3,major genes of close linkage scab-resistance on 3BS,6B and their SSR markers,namely,Xgwm493、Xwmg533、Xgwm389、barc133、barc147、barc102（3BS）、Xgwm644、Xgwm133 and barc101（6BS）,which were used to identify these materials with SSR molecular characterization,the relationship of phenotype and genotype was studied.The results showed that the 15 resistant materials were carried with corresponding resistance genes on 3BS and 6Bs in 25 resistant materials in field scab resistance evaluation,the 15 resistant materials performed unification of phenotype and genotype,the 10 resistant materials were not carried with the corresponding resistance genes on 3BS and 6BS,the 18 susceptible materials were carried with the corresponding resistance genes on 3BS and 6BS.
参考文献/References:
[1]陆维中，程顺和，王裕中.小麦赤霉病研究[M]北京：科学技术出版社，2001:114-115. [2]张勇，张佰桥，高德荣，等.小麦抗病新材料S42抗赤霉病性的主基因+多基因遗传分析[J].江苏农业学报,2005,21(4):272-276. [3]高力，陈力，周立人，等.小麦品种望水白的抗赤霉病性遗传分析[J].麦类作物学报，2005，25（5）：5-9. [4]Zhang Xu,Zhou Miaoping,Ren Lijuan,et al.Molecular characterization of Fusariumhead blight resistance from wheat varietyWangshuibai[J].Euphytica,2004,139:59-64. [5]余桂红,任丽娟,马鸿翔,等.分子标记在小麦抗赤霉病辅助育种中的应用[J].江苏农业学报,２００６,２２（３）:189-191. [6]Anderson J A,Stack R W,Liu S,et al.DNA markers for Fusariumhead blight resistance QTL in two wheat populations[J].Theor Appl Genet,2001,102:1164-1168. [7]周淼平,任丽娟,张旭,等.3B染色体短臂小麦赤霉病抗性主效QTL的分析[J].遗传学报,2003,30(6):571-576. [8]盖钧镒，章元明，王建康.植物数量性状遗传体系[M].北京：科学出版社，2003：120-126. [9]林一波，杨竹平，吴兆苏.不同地理来源抗赤霉病小麦品种的抗性遗传分析[J].上海农业学报，1992，8（1）：31-36. [10]Zhou W C,Kolb F L,Bai G H,et al.Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers[J].Genome,2002,45:719-727. [11]Buerstmayr H,Lemmens M,Hartl L,et al.Molecular mapping of QTLs for Fusariumhead blight resistance in spring wheatⅠ.Resistance to fungal spread(TypeⅡresistance)[J].Theor Appl Genet,2002,104:84-91. [12]Shen X,Zhou M,Lu W,et al.Detection of Fusariumhead blight resistance QTL in a wheat population using bulked segregant analysis[J].Theor Appl Genet,2003,106:1041-1047.
相似文献/References:
[1]郑茂波,丁海燕,谢玉锋,等.黑龙江春小麦品种对温度反应特性的研究[J].黑龙江农业科学,2014,(07):1.
ZHENG Mao-bo,DING Hai-yan,XIE Yu-feng,et al.Study on the Response Characteristics ofTemperature on Heilongjiang Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):1.
[2]车京玉.不同土壤类型的土壤肥力及含水量对春小麦产量的影响[J].黑龙江农业科学,2011,(03):41.
CHE Jing-yu.Effect of Soil Fertility and Soil Moisture Content ofDifferent Soil Types on Spring Wheat Yield[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):41.
[3]邵立刚,刘宁涛.春小麦主要农艺性状配合力分析[J].黑龙江农业科学,2011,(01):1.
SHAO Li-gang,LIU Ning-tao.Analysis of Combining Ability on Main AgronomicCharacters in Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(11):1.
[4]郭怡璠,张宏纪,孙 岩,等.春小麦空间诱变SP2的SSR标记变异分析[J].黑龙江农业科学,2010,(07):9.
GUO Yi-fan,ZHANG Hong-ji,SUN Yan,et al.Variance Analysis of Microsatellite Markers in Spring Wheat SP2 Induced by Space Flight[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(11):9.
[5]朱广芬.互助县浅山地区春小麦品种适应性筛选试验[J].黑龙江农业科学,2010,(07):43.
[6]杨先卿,孙 岩,刘东军,等.相同HMW-GS组成春小麦相关亚基形成和积累规律的研究[J].黑龙江农业科学,2010,(06):16.
YANG Xian-qing,SUN Yan,LIU Dong-jun,et al.Study on the Formation and Accumulation of HMW-GS in Spring Wheat with the Same HMW-GS[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(11):16.
[7]徐 充,黄春艳.75%苯磺隆水分散粒剂防除春小麦田阔叶杂草田间药效评价[J].黑龙江农业科学,2014,(01):50.
XU Chong,HUANG Chun-yan.Field Efficacy Evaluate of 75% Tribenuron-methyl WDG on Broadleaf Weeds Control in Spring Wheat Field[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(11):50.
[8]孙连发,祁适雨,肖志敏,等. 光照方式及时间对不同光反应型春小麦生育进程影响的研究[J].黑龙江农业科学,1994,(01):13.[doi:10.11942/j.issn1002-2767.1994.01.0013]
Sun Lianfa,Qi shiyu,Xiao Zhimin and Liu Wei.[J].HEILONGJIANG AGRICULTURAL SCIENCES,1994,(11):13.[doi:10.11942/j.issn1002-2767.1994.01.0013]
[9]盖德安. 旱灌春小麦需氮磷钾量及高产经济施肥技术研究[J].黑龙江农业科学,1994,(01):24.[doi:10.11942/j.issn1002-2767.1994.01.0024]
Gai Dean.[J].HEILONGJIANG AGRICULTURAL SCIENCES,1994,(11):24.[doi:10.11942/j.issn1002-2767.1994.01.0024]
[10]金正勋,李卓夫,孙艳丽,等. 春小麦高蛋白与高产结合的可能性及途径分析[J].黑龙江农业科学,1994,(04):8.[doi:10.11942/j.issn1002-2767.1994.04.0008]
Jin Zhengxun et al..[J].HEILONGJIANG AGRICULTURAL SCIENCES,1994,(11):8.[doi:10.11942/j.issn1002-2767.1994.04.0008]
备注/Memo
基金项目：国家小麦现代产业技术体系资助项目(CARS-03)