LIU Xiao-chen,CAO Jun-mai,CHEN Yan-yun,et al.Explants Selection and Callus Induction of Apocynum venetum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):24-26.
罗布麻外植体的筛选及愈伤组织诱导
- Title:
- Explants Selection and Callus Induction of Apocynum venetum L.
- 文章编号:
- 1002-2767(2012)09-0024-03
- Keywords:
- Apocynum venetum L.; medium; explant; callus
- 分类号:
- S563.7
- 文献标志码:
- A
- 摘要:
- 以罗布麻带芽茎段、茎段、幼根为外植体,探讨不同培养基、不同激素配比对罗布麻愈伤组织形成的影响。结果表明:适宜的罗布麻外植体为带芽茎段;适宜的培养基为MS培养基;适宜愈伤组织诱导的激素配比为6-BA0.6 mg·L-1+NAA0.5 mg·L-1,其愈伤组织诱导率达到100%。
- Abstract:
- The effect of different mediums and different hormone combinations on callus formation of Apocynum venetum L. was investigated using stems with a bud,stem and root of Apocynum venetum L. as explants.The results showed that the suitable explant was stem with a bud,the suitable medium for callus induction was:MS+6-BA0.6 mg·L-1+NAA0.5 mg·L-1,in which the callus induction rate reached 100%.
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备注/Memo
基金项目:“十一五”国家科技支撑计划资助项目(2011BAC0703)