WU Jian-zhong,HUANG Wen-gong,ZHAO Qian,et al.Polyacrylamide Gel Electrophorus Used in Flax SSR Marker[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):18-20.
聚丙烯酰胺凝胶电泳检测亚麻SSR标记
- Title:
- Polyacrylamide Gel Electrophorus Used in Flax SSR Marker
- 文章编号:
- 1002-2767（2012）09-0018-03
- 分类号:
- S563.2
- 文献标志码:
- A
- 摘要:
- 以亚麻为试验材料，提取亚麻基因组DNA，选择多态性SSR标记，并将标记产物进行聚丙烯酰胺凝胶电泳，最后硝酸银染色，建立了一套有效、方便的应用于亚麻SSR标记的聚丙烯酰胺凝胶电泳银染检测方法。
- Abstract:
- Taking flax as the test material,extracting flax genome DNA,selecting the polymorphic SSR markers,PCR products were electrophoresised in the polyacrylamide gel,and finally they were stained with silver nitrate.An effective,simple and quick PAGE silver staining method was built by flax SSR markers.
参考文献/References:
[1]
汪由，吴禹，李兆波，等
.SSR
分子标记及其在农作物育种中的应用
[J].
农业科技与装备，
2011
，
2(2)
：
17
-
21.
[2]宋显军，张伟，曹萍，等.大豆SSR标记PAGE银染方法的改进[J].安徽农业科学，2006，34(16)：3922-3923. [3]张芳，权军利，单卫星.聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法改良[J].安徽农业科学，2011，39(27):16606-16607. [4]吴建忠.亚麻全基因组DNA的提取及分析[J].黑龙江农业科学,2011(7):18-19. [5]Ali S,Müller C R.DNA fingerprinting by oligonucleotideprobes specific for simple repeats[J].Hum.Genet.，1986(74)：239-243. [6]Wu Kunsheng,Steven D Tanksley.Abundance,polymorphism and genetic mapping of microsatellites in rice[J].Mol Gen Genet，1993，241(1-2)：225-235. [7]Mahinur S Akkaya,Arvind A Bhagwat,Perry B Cregan.Length polymorphisms of simple sequence repeat DNA in soybean[J].Genetics,1992,132(4):1131-1139. [8]Bell C J,Ecken J R.Assignment of 30 microsatellite loci tothe linkage map of Arabidopsis[J].Genomics,1994,19:137-144. [9]Wang Z,Weber J L,Zhong G,et al.Survey of plantshort tandem DNA repeats[J].Theor. Appl. Genet.,1994,88(1):1-6. [10]Morgante M,Olivieri A M.PCR-amplified microsatellites as markers in plant genetics[J].The Plant J.,1993,3:175-182. [11]王斌，张建平，党占海，等.亚麻SSR-PCR优化体系的建立[J].甘肃农业大学学报，2010，4(45)：69-73.
相似文献/References:
[1]姚玉波,吴广文,黄文功,等.浅析黑龙江省亚麻耕作栽培技术的发展[J].黑龙江农业科学,2014,(04):147.
YAO Yu bo,WU Guang wen,HUANG Wen gong,et al.Study on the Development of Tillage and Cultivation Techniques of Flax in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(09):147.
[2]吴建忠.利用正交设计优化亚麻SRAP-PCR反应体系[J].黑龙江农业科学,2012,(03):30.
WU Jian-zhong.Optimization of SRAP-PCR System for Flax Based on Orthogonal Design[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):30.
[3]吴建忠,赵 茜,关凤芝.11个亚麻品种的产量特征分析[J].黑龙江农业科学,2012,(10):18.
WU Jian-zhong,ZHAO Qian,GUAN Feng-zhi.Yield Characteristics Analysis of Eleven Flax Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):18.
[4]杨学,关凤芝,李柱刚,等.亚麻种质创新的研究现状[J].黑龙江农业科学,2011,(03):8.
YANG Xue,GUAN Feng-zhi,LI Zhu-gang,et al.Advances in Research of Flax Germplasm Innovation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):8.
[5]赵东升.亚麻耐盐碱ISSR标记引物筛选的研究[J].黑龙江农业科学,2013,(08):12.
ZHAO Dong-sheng.Reaction of ISSR Molecular Marker Primers Screening for Saline-alkali Tolerant Flax[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(09):12.
[6]杨 学,赵念力,关凤芝,等.不同药剂对亚麻炭疽病的防治效果[J].黑龙江农业科学,2011,(04):76.
YANG Xue,ZHAO Nian-li,GUAN Feng-zhi,et al.Control Efficacy of Different Chemical on the Flax Anthracnose[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):76.
[7]赵东升.亚麻耐盐碱品种筛选的研究[J].黑龙江农业科学,2011,(07):12.
ZHAO Dong-sheng.Screening Salt-alkali Resistant Varieties of Flax[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):12.
[8]吴建忠.亚麻全基因组DNA的提取及分析[J].黑龙江农业科学,2011,(07):18.
WU Jian-zhong.Extraction and Analysis of DNA in Flax Genomic[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):18.
[9]姜卫东.云南省元谋县亚麻冬季栽培技术[J].黑龙江农业科学,2010,(07):185.
JIANG Wei-dong.Cultivation Techniques of Flax in Winter in Yuanmou County of Yunnan Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(09):185.
[10]黄文功.亚麻种质资源的RAPD分析[J].黑龙江农业科学,2011,(08):11.
HUANG Wen-gong.RAPD Analysis of Flax Germplasm[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):11.
备注/Memo
第一作者简介：吴建忠（1983-），男，内蒙古自治区乌兰察布市人，硕士，研究实习员，从事亚麻生物技术及遗传育种研究。E-mail：wujianzhong176@163.com。