SHU Guo-biao,WANG Zu-tao,YANG Liu,et al.The Anatomical Structure of Pseudobulb of Bletilla striata?Adapt to Nutrient Accumulation and Fungus Resistance[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):121-125.
适应于营养积累和防止真菌侵染的白芨块茎结构
- Title:
- The Anatomical Structure of Pseudobulb of Bletilla striata?Adapt to Nutrient Accumulation and Fungus Resistance
- 文章编号:
- 1002-2767（2012）08-0121-05
- Keywords:
- Bletilla striata; fungus; tuber; pseudobulb; polysaccharide
- 分类号:
- S567.23+9
- 文献标志码:
- A
- 摘要:
- 兰科植物白芨的块茎是我国传统中药，该文对白芨块茎的显微结构进行了观察，同时为确认白芨块茎内是否有真菌侵染，进行了内生真菌分离实验。结果表明：白芨的块茎具有茎的基本结构特征，为单子叶植物典型的茎结构，具一层表皮细胞，外被发达的角质层；表皮下有一层细胞壁加厚的下皮层，之内则为由薄壁组织构成的基本组织，多数小维管束星散分布于基本组织中。基本组织中有两种类型的薄壁组织，大的储水细胞和小的贮藏细胞，储水细胞内积聚水和无机盐，具针晶束；贮藏细胞内积聚有较大的颗粒，经PAS反应显示为多糖类物质；两种细胞有规则地分布。真菌分离实验和显微结构观察均表明白芨块茎中没有任何真菌侵染。白芨块茎中储水细胞和贮藏细胞很好地适应了它作为营养繁殖器官的需要，而表皮外发达的角质层以及表皮细胞加厚的细胞壁则是适应于地下生活的需要，有效地阻止了真菌菌丝的侵入。
- Abstract:
- Bletilla striata(Thunb.)Reichb.f.of Orchidaceae is widely used as traditional medicine and the function part is the underground pseudobuld(usually called tuber)of the plant.In this study,the anatomy of the pseudobuld of B.striata?was studied by paraffin section.The pseudobulb of B.striata had a typical monocot stem structure,consisting of an epidermis outside and large ground tissue inside with scattered vascular bundles.The epidermal cells had thickened walls and a smooth thick cuticle layer was formed outside of the epidermis.The adjacent cell layer developed into hypodermis by cell wall thickening.The ground tissue is made up of two types of parenchyma cells,large and small starch grain containing assimilatory cells and larger,circular,water-storage cells.No fungus was found in pseudobulb of B.striata?and the PDA medium culture proved the resistance of the pseudobulb from the fungus.The thick walls of the epidermal cells and the outside cuticle layer were the reason of its resistance to fungi
参考文献/References:
[1] 中国植物志编委会 . 中国植物志 ( 第十八卷 ) 兰科 (2)[M]. 北京 : 科学出版社 ,1999 ： 50. [2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010：95. [3]郑传胜,冯敢生,周汝明,等.中药白芨的新用途[J].中华肿瘤杂志,1996（18）:305-307. [4]周高龙.白芨用于血管瘤[J].中医杂志,1997,38(6):326. [5]杨俊慧,孟庆军,李建东,等.利用组织培养法繁殖白芨[J].山东科学，2002,15(1):13-16. [6]彭丽丽,刘祥东,刘华,等.白芨的组培快繁（简报）[J].中国野生植物资源,2003,23(5):65. [7]曾宋君,黄向力,陈之林,等.白及的无菌播种和组织培养研究[J].中药材,2004,27(9):625-627. [8]余朝秀,李枝林,王玉英.野生白芨组培快繁技术研究[J].西南农业大学学报：自然科学版，2005,27(5):601-604. [9]付志惠,张建霞,李洪林,等.白及种子萌发与快速繁殖技术的研究[J].武汉植物学研究,2006,24(1):80-82. [10]〖ZK(#〗吴华芬,姚宏,刘南祥,等.离体培养丽水野生白芨快速繁殖[J].北方园艺,2008(6):180-182. [11]石云平,李锋,凌征柱.白芨组织培养与快速繁殖技术研究[J].广西农业科学,2009,40(11):1408-1410. [12]袁宁,何俊蓉,何锐,等.白芨组培快繁育苗技术研究[J].西南农业学报,2009,22(3):781-785. [13]赵漫丽,黄春球,李明静,等.添加剂对白芨组培的影响[J].云南农业大学学报,2011,26(6):821-827. [14]张亦诚.白芨的生物特性及栽培技术[J].中药材,2007(10):45. [15]Pridgeon,Crib,Chase，et al.Genera Orchidacearum Volume 4 Epidendroideae(Part one)[M].Landon：Oxford University Press,2005:30-33. [16]郑小珠,杜泉基.中药白芨的显微鉴别[J].广东药学院学报,1994,10(3):169. [17]Krüger P.Die oberiridischen Vegetationsorgane der Orchideen in ihren Beziehungen zu Clima und Standort [M].Flora,1883:435-443,451-459,467-477,499-510,515-523. [18]Weltz M.Zur Anatomie der monandrischen sympodialen Orchideen[M].Heidelberg,1897：1-53. [19]Stern W L，Judd W S.Comparative anatomy and systematics of Catasetinae(Orchidaceae)[J].Botanical Journal of the Linnean Society,2001,136(2):153-178. [20]Yukawa T，Stern W L.Comparative vegetative anatomy and systematics of Cymbidium(Cymbidieae:Orchidaceae)[J].Botanical Journal of the Linnean Society，2002,138(4):383-419. [21]朱小平,吴东儒.白芨多糖分离、纯化、组成及其性质[J].安徽大学学报：自然科学版,1991（4）:87-91.
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备注/Memo
基金项目：云南大学国家级大学生科研创新资助项目(教育部X3108052)；云南大学“211”三期重点学科建设资助项目（21134025）