ZHANG Dao-wei,CHEN Jing,GUO Yu-shuang.Cloning and Sequence Analysis of Trehalose Phosphate Synthase Gene from Sogatella furcifera [J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):14-19.
白背飞虱海藻糖合成酶基因的克隆及序列分析
- Title:
- Cloning and Sequence Analysis of Trehalose Phosphate Synthase Gene from Sogatella furcifera
- 文章编号:
- 1002-2767（2012）05-0014-06
- 分类号:
- S435.112.3；Q78
- 文献标志码:
- A
- 摘要:
- 为进一步深入研究白背飞虱的迁飞能力与能量代谢，利用同源克隆及RACE的方法，从白背飞虱Sogatella furcifera中克隆获得海藻糖合成酶基因(TPS)的全长cDNA序列(Genbank 登录号：JQ013797)，该基因全长为3 278 bp，包含353 bp 的3非编码区域(UTR)和501 bp的5UTR，开放阅读框(ORF)为2 424 bp，编码807个氨基酸。该基因预测的蛋白分子量为90.372 kD，等电点为6.08，有3个预测的糖基化位点，无信号肽及跨膜结构。进化分析结果表明：白背飞虱的海藻糖合成酶与其它昆虫的海藻糖合成酶基因的同源性较高，与褐飞虱相比较同源性高达98%。
- Abstract:
- For the purpose of deep studying the migration ability and energy metabolism of Sogatella furcifera，the Trehalose Phosphate Synthasegene from Sogatella furcifera was cloned by homology cloning strategy and technique of Rapid-Amplification of cDNA Ends(RACE).The full-length cDNA of S.furcifera TPS(SfTPS)was 3 278 bp(Genbank number：JQ 013797)and had an open reading frame(ORF)of 2 424 bp，a 3 UTR(untranslated region)of 353 bp and a 5UTR of 501 bp.The ORF of SfTPS encoded a polypeptide of 807 amino acids with a calculated molecular weight of 90.372 kD，and pl of 6.08.There were three N-glycosylation sites，but no signature peptide and putative transmembrane.A phylogenetic tree results using Blast showed that the amino acid sequence of SfTPS had the greatest similarity to other insects.Compared with Nilaparvata lugens，they were 98% identified.
参考文献/References:
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相似文献/References:
[1]张道伟,陈 静,郭玉双.白背飞虱HSP90全长cDNA的克隆与特性分析[J].黑龙江农业科学,2012,(06):13.
ZHANG Dao-wei,CHEN Jing,GUO Yu-shuang.Cloning and Characterization Analysis of Heat ShockProtein 90 Gene from Sogatella furcifera[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):13.
备注/Memo
第一作者简介：张道伟（1981-），男，河南省内乡县人，博士，讲师，从事昆虫及农业螨类分子生物学研究。E-mail：zhangdw1000@163.com。