MAN Chao-lai,YU Xiao-long.Sequence Analysis of ORF7 Gene of TransmissibleGastroenteritis Virus Isolated from Different Regions[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(08):41-43.
部分地区猪传染性胃肠炎病毒株ORF7基因的序列分析
- Title:
- Sequence Analysis of ORF7 Gene of TransmissibleGastroenteritis Virus Isolated from Different Regions
- 文章编号:
- 1002-2767（2011）08-0041-03
- Keywords:
- pig; transmissible gastroenteritis virus; ORF7 gene; sequence analysis
- 分类号:
- S852.65+1
- 文献标志码:
- A
- 摘要:
- 猪传染性胃肠炎（Porcine Transmissible Gastraenteritis,TGE）是猪的一种高度接触性肠道疾病。以不同地区猪传染性胃肠炎病毒株的ORF7基因为靶序列，序列分析不同地区TGEV病毒株ORF7基因的同源性和进化性关系，序列分析包括同源性比较、进化树分析和碱基替换分析等。结果表明：ORF7基因在TGEV不同毒株间高度保守，但ORF7基因存在腺嘌呤碱基（A）向胸腺嘧啶（T）突变的倾向。通过对TGEV ORF7基因核苷酸序列分析可为深入研究TGEV的毒力变化和流行病学提供参考。
- Abstract:
- Porcine Transmissible gastroenteritis（TGE）is an acute highly contagious disease of pigs caused by transmissible gastroenteritis virus（TGEV）.ORF7gene sequences of several TGEV strains derived from different regions were analyzed for the homology and evolution by Sequence analysis,which including measurement of sequence homology,phylogenetic tree analysis and residue substitution analysis.The results showed that the ORF7 genes were highly conservative between each TGEV strains,but there existed the tendency of adenine nucleotide（A）mutation to thymidine nucleotide（T）.It also showed that the nucleotide sequence analysis could form a base or further study on the virulence and epidemiological study of TGEV infections.
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备注/Memo
基金项目：黑龙江省自然基金重点资助项目（ZJN0602-02）