GONG Na,YANG Tao,XIAO Jun,et al.Study on Callus Induction of Gerbera Jiamesonii Receptacle[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(03):21-23.
非洲菊花托愈伤组织的诱导研究
- Title:
- Study on Callus Induction of Gerbera Jiamesonii Receptacle
- 文章编号:
- 1002-2767（2011）03-0021-03
- Keywords:
- Gerbera jiamesonii; receptacle; callus
- 分类号:
- S682.1+1
- 文献标志码:
- A
- 摘要:
- 以温室生长健壮、无病虫害的非洲菊品种太阳和诺苏娜为材料，选取花托为外植体，研究了2，4-D+6-BA和2，4-D+KT不同浓度对非洲菊愈伤组织诱导的影响。结果表明：太阳接种在MS+2，4-D4.0 mg·L-1+6-BA0.5 mg·L-1培养基中愈伤组织诱导率最高，为65.0%，太阳的外植体35 d左右开始有愈伤组织出现，诺苏娜愈伤组织诱导率低于太阳，其接种后40 d才出现愈伤组织，但愈伤组织诱导率均较低，诺苏娜 接种在MS+2，4-D 4 mg·L-1+6-BA 1.0 mg·L-1培养基中愈伤组织诱导率最高，为45.0%。
- Abstract:
- Taking the Taiyang and the Nuosuna Gerbera jiamesonii without diseases and insect as the materials,and the receptacle was selected as explant,the effects of different concentration of 2，4-D+6-BA and 2，4-D+KT on Gerbera jiamesonii callus induction were studied were studied.The results showed that，the highest inductivity of the Taiyang callus which growing in MS+2，4-D 4.0 mg·L-1+6-BA 0.5 mg·L-1medium was 65.0%. The Taiyang explants began to produce callus after 35 days，and the nuosuna explants produce callus after 40 days，due to its low callus inductivity.And the highest inductivity of the nuosuna callus was 45.0%，in MS+2，4-D 4 mg·L-1+6-BA 1.0 mg·L-1medium.
参考文献/References:
[1]鲁雪华，郭文杰，林勇.几种因素对非洲菊离体培养再生植株的影响( 简报 )[J]. 植物生理学通讯，1999?（10）： 372 - 374. [2]叶华.非洲菊组织培养和转基因研究概况[J].思茅师范高等专科学校学报.2010（3）：10-12 [3]何遐义，张秀文，石和平，等.玉米幼胚培养及其影响因素的研究[J].中山大学学报论丛，1996（2）：19-25. [4]Carvalho C H S，Bohorova N，Bordallo P N，et al.TypeⅡcallus pro-duction and plant regeneration in tropical maize genotypes[J].Plant Cell Rep.，1997，17：73-76.
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备注/Memo
第一作者简介：龚娜（1982-），女，辽宁省沈阳市人，硕士，助理研究员，从事植物组织培养及分子育种等研究。E-mail：doll52133@163.com。