YANG Hong-yi,ZHOU Yang-yang.Study on the Antagonistic Activity of Trichoderma viride andBacillus subtilis against Fusarium acuminatum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(12):57-59.
绿色木霉和枯草芽孢杆菌对玉竹锐顶镰孢菌的拮抗作用研究
- Title:
- Study on the Antagonistic Activity of Trichoderma viride andBacillus subtilis against Fusarium acuminatum
- 文章编号:
- 1002-2767(2010)12-0057-03
- 分类号:
- S435.67
- 文献标志码:
- A
- 摘要:
- 采用平板对峙培养法、对扣培养法和圆盘滤膜法研究绿色木霉和枯草芽孢杆菌对玉竹锐顶镰孢菌的拮抗作用。结果表明:木霉和枯草芽孢杆菌对锐顶镰孢菌菌丝生长具有一定抑制作用,木霉的抑制效果较差,枯草芽孢杆菌的抑制效果较好,其中枯草芽孢杆菌菌株Dc10的抑制作用最强,其非挥发性代谢产物对病原菌的抑制率可达100%。
- Abstract:
- The antagonistic activity of Trichoderma viride and Bacillus subtilis against Fusarium acuminatum was analyzed by competitive culture,culture on facing each other,and filter membrane culture in disk,respectively.The results indicated that both T. viride and B.subtilis had the efficacy to inhibit the development of mycelium of F.acuminatum.The efficacy of inhibition of T. viride was low,but B.subtilis had strong inhibition capability to F.acuminatum.It was the best for the efficacy of inhibition of B.subtilis Dc10,its inhibition for non-volatile metabolize product could up to 100%.
参考文献/References:
[1]国家药典委员会.中国药典(2005年版一部)[M].北京:化学工业出版社,2005:57-58. [2]高苇,李宝聚,孙军德,等.绿色木霉对黄瓜立枯丝核菌和尖孢镰刀菌的拮抗作用[J].中国蔬菜,2008(6):9-12. [3]宋漳,陈辉.绿色木霉对土传病原真菌的体外拮抗作用[J].福建林学院学报,2002,22(3):219-222. [4]Dennis C,Webster J.Antagonistic properties of species-groups of Trichodermaproduction of non-volatile antibiotics[J].Transactions of the British MycologicalSociety,1971,57:25-39. [5]Whipps J M,Magan N.Effects of nutrient status and water potential of media on fungal growth and antagonist-pathogen interactions[J].Bulletin OEEPP/EPPO Bulletin,1987,17:581-591. [6]毛腾霄.枯草芽孢杆菌BS-8D防治玉米纹枯病(Rhizoctonia solani)的研究[D].重庆:四川农业大学,2006.
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备注/Memo
基金项目: 黑龙江省博士后基金资助项目(LBH-Z08252)