ZHANG Xi-tai,SONG Jiu-ying,XIE Shu-qin.Reproduce Shoot from Stipule of Strawberry in Vitro[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(04):12-13.[doi:10.11942/j.issn1002-2767.2008.04.0012]
利用草莓试管苗托叶再生植株
- Title:
- Reproduce Shoot from Stipule of Strawberry in Vitro
- 文章编号:
- 1002-2767(2008)04-0012-02
- Keywords:
- straw ber ry ; straw berry in vitro ; stipule ; regenera tion plantlet
- 分类号:
- S668 .4
- 文献标志码:
- A
- 摘要:
- 利用脱毒草莓试管苗叶柄基部的托叶在“ MS +6 -BA 1.0～ 2 .0 mg ·L -1”培养基上不经过愈伤组织阶段,直接再生了草莓试管植株, 植株再生率达100 %, 每个托叶最高分化试管苗数达12 .5 株;提高了脱毒草莓试管苗的继代增殖倍数, 提高了脱毒草莓试管种质材料的利用率, 降低了脱毒草莓试管苗的成本。
- Abstract:
- The shoo ts have been repro duced directly fr om stipules o f virus-elimination st rawber ry in the“ MS +6-BA 1.0～ 2 .0 mg ·L-1”medium in vit ro , the percentage of sho ots rege ner ation was 100% and the hig hest numberof shoo ts reproduced from stipule s o f virus-eliminatio n st rawber ry w as 12 .5(stub/ stipule);the reproduction pe rcentageo f virus-elimination str awber ry in vitro w as bee n increased, the materia l utiliza tion of virus-eliminatio nstraw be rry in vitro w as increased and the pro ductio n costs w as reduced.
参考文献/References:
[ 1] 牛建新, 将迪军, 高守强.草莓茎叶切块愈伤组织试管苗生长结果调查[ J] .北方园艺, 1997(6):53-54 .
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备注/Memo
基金项目:邯郸市科技攻关项目(2003055)