[1]刘双奇,邹德堂,孟英,等.农杆菌介导北方粳稻转化体系的研究[J].黑龙江农业科学,2008,(04):6-8.[doi:10.11942/j.issn1002-2767.2008.04.0006]
　LIU Shuang-qi,ZOU De-tang,MENG Ying,et al.Establishment of Transformation System Mediated by AgrobacteriumTumefaciens in Grain-oryza of North of China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(04):6-8.[doi:10.11942/j.issn1002-2767.2008.04.0006]
点击复制

农杆菌介导北方粳稻转化体系的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2008年04 页码: 6-8 栏目: 出版日期: 2008-07-10
Title:
Establishment of Transformation System Mediated by AgrobacteriumTumefaciens in Grain-oryza of North of China
文章编号:
1002-2767(2008)04-0006-03
作者:
刘双奇1 ;  邹德堂1 ;  孟英2 ;  卢泳全3 ;  卞景阳2
1 .东北农业大学农学院, 哈尔滨150030 ;
2 .黑龙江省农业科学院耕作栽培研究所, 哈尔滨150086 ;
3 .黑龙江省农业科学院生物技术研究所, 哈尔滨150086
Author(s):
LIU Shuang-qi1 ; ZOU De-tang1 ;  MENG Ying2 ;  LU Yong-quan2 ; BIAN Jing-yang2
1 .Agro nomy College of No rtheast Agricul tural University , Harbin 150030 ;
2 .C rop Tillage andCul tivat ion Insti tute of Heilongjiang Academy of A gricul tural Sciences , Harbin 150086 ;
3 .Biotechnological Research Insti tute of Heilo ng jiang Academy of Ag ricultural Sciences , Harbin150086
关键词:
粳稻; 农杆菌; 遗传转化; GUS 瞬时表达率
Keywords:
Japonica rice ; Ag robacte rium tumefaciens ; gene tic transforma tion ; transient expression of GUS gene
分类号:
Q933 ;S513
DOI:
10.11942/j.issn1002-2767.2008.04.0006
文献标志码:
A
摘要:
通过农杆菌介导的方法将Bar-Bt 基因(抗除草剂基因和抗虫基因构建在同一载体上的双基因)整合到北方优质粳稻龙稻6 号、松粳9 号等品种中;分别以供试品种的幼胚和成熟胚为外植体诱导愈伤组织, 携带有Bar-Bt 基因双元载体PCAM BIA1301 号的根癌农杆菌EHA105 为载体, GUS 基因的瞬时表达率为衡量指标, 对影响农杆菌介导的龙稻6 号、松粳9 号等品种的转化效率的主要因素进行了研究。结果表明:农杆菌侵染愈伤组织的菌液浓度以D(600nm)= 0 .8 ～ 1.0 较为适宜, 最佳共培养时间为2 ～ 3 d , 预培养时间为5 d , 共培养培养基中添加100 μmo l·L-1 乙酰丁香酮的转化效率有明显提高。
Abstract:
With juvenile embry o o f g rain-ory za of No rth of China as e xplant , Ag ro bac te rium tumefaciens strainEHA105 car rying Tipla smid PCAMBIA1301 w ith Bar-Bt gene a s vector , and the tr ansient expressio n rate of GUSgene as measuring index , main facto rs that affected tra nsfo rmatio n efficiency of g rain-or yza o f No rth of China mediated by Ag robacterium tumefaciens w ere studied in this a rticle .Results showed tha t the proper concentratio n ofbac teria solution to infect callus w as D(600nm)= 0 .8～ 1 .0 , the most suitable co-cultiva tion time w as 2 ～ 3 days ,pre-cultiv atio n time was 5 day s, and addition o f 100 μmo l·L-1 Aceto sy ring one im pr oved transfo rma tion efficiencyconsider ably.

参考文献/References:

[ 1] 程继鸿, 王鸣, 何玉科, 等.植物抗虫基因工程研究进展[ J] .北京农学院学报, 2000 , 15(1):56-58 .

[ 2] 朱玉贤.植物基因的克隆与转化研究进展[ J] .河北科技大学学报, 1999 , 20(2):5-9 .
[ 3] Jeff erson R A .As saying chimeric genes in p lan ts ;the GUSgene fu si on syst em[ J] .Plan t Mol Rep ., 1987 , 5 :387-405 .
[ 4] 王关林, 方宏筠.植物基因工程的原理与方法[ M] .北京:科学出版社, 1998 .

相似文献/References:

[1]许健,梁虹,曲忠诚,等.钾肥对黑龙江省西部水稻产量及构成因素的影响[J].黑龙江农业科学,2014,(05):52.
　XU Jian,LIANG Hong,QU Zhong cheng,et al.Effects of Potassium Fertilizer Amount on Rice Yield and Component Factors in the Western of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):52.
[2]陈书强.两种穗型品种粒重和结实率在穗上不同粒位的分布差异[J].黑龙江农业科学,2014,(04):18.
　CHEN Shu qiang.Difference Analysis on Grain Weight and Seed Setting Rate of Different Grain Positions at Panicle Between Two Panicle Types of Japonica Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):18.
[3]孙玉友.北方粳稻产量及产量构成因素相关特性研究[J].黑龙江农业科学,2012,(05):6.
　SUN Yu-you.Correlation Characters Study on the Yield and Yield Components of Japonica Rice in Northern China[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):6.
[4]谭贺,曾宪楠,夏天舒,等.高温对寒地粳稻萌发影响的研究[J].黑龙江农业科学,2012,(04):32.
　TAN He,ZENG Xian-nan,XIA Tian-shu,et al.Effects of High Temperature on Germination of Japonica Rice Seed in Cold Area[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):32.
[5]夏天舒,赵宏亮,谭 贺,等.中国北方粳稻资源数据库平台系统的构建[J].黑龙江农业科学,2012,(10):147.
　XIA Tian-shu,ZHAO Hong-liang,TAN He,et al.Construction of North China JaponicaRice Resources Database Platform System[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(04):147.
[6]马 波,刘传增,胡继芳,等.寒地粳稻耐盐碱种质资源筛选[J].黑龙江农业科学,2011,(01):6.
　MA Bo,LIU Chuan-zeng,HU Ji-fang,et al.Screening and Selecting of JaponicaGermplasm withSaline-Alkali Tolerance in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):6.
[7]贺玺强,徐恒戬.大白菜再生体系和转基因影响因素的研究[J].黑龙江农业科学,2013,(09):9.
　HE Xi-qiang,XU Heng-jian.Study on Regeneration System and Transgenic Impact Factors of Chinese Cabbage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(04):9.
[8]李 辉,戴常军,张瑞英,等.储藏时间对东北粳稻品质影响的研究[J].黑龙江农业科学,2011,(10):94.
　LI Hui,DAI Chang-jun,ZHANG Rui-ying,et al.Effect of Storage Time on the Quality of Northeastern Japonica Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(04):94.
[9]孟庆虹,李霞辉,卢淑雯,等.黑龙江省粳稻品种的品质现状与评价[J].黑龙江农业科学,2010,(06):108.
　MENG Qing-hong,LI Xia-hui,LU Shu-wen,et al.Status and Evaluation of Japonica Rice Varieties Eating Quality in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(04):108.
[10]高 尚,李红宇,潘世驹,等.粳稻幼苗前期耐盐碱性鉴定方法研究[J].黑龙江农业科学,2014,(12):36.
　GAO Shang,LI Hong-yu,PAN Shi-ju,et al.Study on Identification Methods of Alkali-saline Tolerance at Early Seedling Stage for Japonica Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(04):36.

备注/Memo

第一作者简介:刘双奇(1982-),男, 江苏人, 农学硕士, 从事水稻遗传育种研究。E -mail:pi rat e.y @163 .com 。

更新日期/Last Update: 2016-12-14