[1]王琳,王晓麟,蔺博超,等.银粉背蕨组织培养及无性系建立的研究[J].黑龙江农业科学,2008,(03):8-10.[doi:10.11942/j.issn1002-2767.2008.03.0008]
　WANG Lin,WANG Xiao-l in,LIN Bo-chao,et al.Tissue Culture and Clone Construction ofAleuritopteris argentea var .argentea[J].HEILONGJIANG AGRICULTURAL SCIENCES,2008,(03):8-10.[doi:10.11942/j.issn1002-2767.2008.03.0008]
点击复制

银粉背蕨组织培养及无性系建立的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2008年03 页码: 8-10 栏目: 出版日期: 2008-03-10
Title:
Tissue Culture and Clone Construction ofAleuritopteris argentea var .argentea
文章编号:
1002-2767(2008)03-0008-03
作者:
王琳;  王晓麟;  蔺博超;  孙彗超;  姜长阳
辽宁师范大学生命科学学院, 大连116029
Author(s):
WANG Lin ; WANG Xiao-l in ; LIN Bo-chao ;  SUN Hui-chao ;  JIANG Chang-yang
Life Science College of Liao ning N ormal University ,Dalian 116029
关键词:
银粉背蕨; 组织培养; 无性系
Keywords:
A leuritopteris argentea var .argentea ; tissue culture ; clo ne
分类号:
Q813 .1
DOI:
10.11942/j.issn1002-2767.2008.03.0008
文献标志码:
A
摘要:
以银粉背蕨叶柄为材料, 成功地诱导了愈伤组织, 建立起无性系。结果表明:1/2 MS +NH4 H2 PO4 200 mg·L-1 +BA 0.3 mg ·L-1 +IBA 0 .4 mg·L-1 是诱导叶柄形成愈伤组织的理想培养基;1/2 MS 是颗粒状愈伤组织分化培养的理想培养基;1/3 MS + IAA 0 .4 mg ·L-1 是试管苗生根培养的理想培养基;炉灰渣是试管苗移栽的理想基质;移植后的试管苗生长旺盛、根系发达, 秋末收获单株干草重量比野生植株增加14.6%。
Abstract:
Callus induction and plant reg ene ratio n from the petio le in Aleuritopteris argentea var .argentea wererepor ted in this re search .The re sults showed that the medium 1/ 2 MS +NH4H 2 PO4 200 mg ·L1 +BA 0.3 mg·L1+IBA 0.4 mg ·L1 wa s the best for inducing callus , medium 1/ 2 MS w as the most suitable fo r bud differe ntiation ,medium 1/3 MS + IAA 0 .4 mg ·L1 w as the optimum for roo ting .The ideal transplantation matrix fo r tube plantletswa s the furnace a shes .The tr ansplanted plantlets in the field g rew vig o rously and had a w ell develo ped r oo tsy stem .The hay w eig ht of the single plant w as 14 .6 % more than that of the wild plant.

参考文献/References:

[ 1] 中国科学院中国植物志编辑委员会.中国植物志[ 第三卷(第一分册)] [ M] .北京:科学出版社,1990 :154-155 .

[ 2] 李书心.辽宁植物志(上册)[ M] .沈阳:辽宁科学技术出版社,1988 :39-40 .
[ 3] 江苏新医学院.中药大辞典[ M] .上海:上海人民出版社, 1977 .
[ 4] 李文安, 王玉琴.狼尾蕨的离体培养[ J] .植物生理学通讯,1993 , 29(3):44 .
[ 5] 尹怀约.贯众叶片愈伤组织的诱导和植株再生[ J] .植物生理学通讯, 1989 , 25(4):39-45 .
[ 6] 郑若仙.彩叶凤尾蕨孢子离体繁殖与组织培养[ J] .植物杂志,1992 , 28(2):8 .
[ 7] 金建平, 兰涛.皱叶肾蕨卷曲叶尖的离体培养[ J] .植物生理学通讯, 1992 , 28(5):359-360 .
[ 8] Mat sum ot o S adamu .S pecies Ecological Study on Reproductive S yst em s and S peciat ion of Cy rtomium f alcatum C om plex(Dryop teridaceae)in Japanese A rchipelago[ J] .Ann al s of theT sukub a Botanical Garden , 2003 , 22 :1-24 .
[ 9] 黄韶玲, 张洁莲.鹿角蕨的组织培养[ J] .植物生理学通讯,1993 , 29(1):25 .
[ 10] 秦廷豪, 邹宗兰.鸟巢蕨的组织培养[ J] .植物生理学通讯,2004 , 40(3):349 .
[ 11] 彭晓明, 曾宋君.铁线蕨的组织培养及植株再生[ J] .植物生理学通讯, 2004 , 40(5):575 .
[ 12] Amb rosi o, S and ra Tereza, Nat oniel Fran klin de M elo .Int eraction betw een su crose and pH during in vi t ro cul tu re of Nephrolepis bi serrat a(Sw .) Sch ott (Pt eridophy ta)[ J] .Act a BotBra, 2004 , 18(4):809-813 .
[ 13] Camloha M , Gogala N , Rode J .Plant regernarati on f rom leafex plant s of the Platy cerium bi fu rcatum in vi t ro[ J] .Scient iaH orti culture , 1994 , 56 :257-266 .
[ 14] 韦景枫, 匡世秀, 程友忠, 等.贵州六种观赏蕨的组织培养初报[ J] .贵州林业科技, 2007 , 35(1):55-57 .
[ 15] 姜长阳.培养基琼脂用量计算的商榷[ J] .植物生理学通讯,1992 , 28(2):155 .
[ 16] 胡尚连, 王丹.植物生物技术[ M] .西安:西安交通大学出版社, 2004 :35-40 .

相似文献/References:

[1]孙岩,张宏纪,王广金,等.航天处理对小麦组织培养效果的研究[J].黑龙江农业科学,2014,(11):1.
　SUN Yan,ZHANG Hong-ji,WANG Guang-jin,et al.Study on the Effect of Space Treatment on Wheat Tissue Culture[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):1.
[2]王碧才,丰 锋.文心兰不定芽生根培养基配方的优化与移栽基质筛选[J].黑龙江农业科学,2014,(11):12.
　WANG Bi-cai,FENG Feng.Optimization of Rooting Medium and Screening of Transplanting Medium of Oncidium[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):12.
[3]曹天旭,宿肇辉,韩超慧.铁皮石斛组培快繁技术的研究[J].黑龙江农业科学,2014,(05):30.
　CAO Tian-xu,SU Zhao-hui,HAN Chao-hui.Study on Tissue Culture and Rapid Propagation of Dendrobium candidum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):30.
[4]唐文杰,王玉,周泉澄,等.马齿苋组培与耐盐筛选[J].黑龙江农业科学,2013,(03):7.
　TANG Wen-jie,WANG Yu,ZHOU Quan-cheng,et al.Tissue Culture and Salt Resistance Selection of Portulaca oleracea L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):7.
[5]尹相博,于咏梅,于立芝.红树莓组织培养研究进展[J].黑龙江农业科学,2013,(02):140.
　YIN Xiang-bo,YU Yong-mei,YU Li-zhi.Review of Progress in Tissue Culture of Red Raspberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):140.
[6]陈荣珠,沈应柏,罗晓芳,等.连香树组织培养及植株再生体系的初步研究[J].黑龙江农业科学,2012,(01):16.
　CHEN Rong-zhu,SHEN Ying-bai,LUO Xiao-fang,et al.Research on Tissue Culture and Plant Regeneration of Cercidiphyllum japonicum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):16.
[7]曹羽.香花槐组织培养研究现状[J].黑龙江农业科学,2012,(04):142.
　CAO Yu.Research Advance in Tissue Culture of Robinia pseudoacaia ‘Idaho’[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):142.
[8]张瑜,柏彦伸,孙靖靓,等.活血丹子叶无性系建立的研究[J].黑龙江农业科学,2012,(11):21.
　ZHANG Yu,BAI Yan-shen,SUN Jing-liang,et al.Study on Establishment of Clone for Giechoma longituba[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):21.
[9]时群,何贵整,陈乃明,等.木薯产业化组培育苗技术研究[J].黑龙江农业科学,2012,(11):25.
　SHI Qun,HE Gui-zheng,CHEN Nai-ming,et al.Study on Industrialization Tissue CultureSeedlings Technology of Cassava[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):25.
[10]张元国,李 芳,包艳存,等.芦笋全雄品种WF-8茎尖组培快繁技术研究[J].黑龙江农业科学,2012,(12):21.
　ZHANG Yuan-guo,LI Fang,BAO Yan-cun,et al.Study on Tissus Culture Rapid Reproduction Technology of Stem Tip of All Male Asparagus Cultivar WF-8[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):21.

备注/Memo

第一作者简介:王琳(1986-), 女, 辽宁沈阳人, 辽宁师范大学生命科学学院本科在读。T el:13842696809 。

更新日期/Last Update: 2016-12-13