JIN Yu,WEN Jing-zhi.Advances on Methods for the Detection of Plant Virus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2005,(03):37-40.[doi:10.11942/j.issn1002-2767.2005.03.0037]
植物病毒检测方法研究进展
- Title:
- Advances on Methods for the Detection of Plant Virus
- 文章编号:
- 1002 -2767(2005)03 -0037 -04
- Keywords:
- plant vi rus ; detection ; advances
- 分类号:
- S432 .41
- 文献标志码:
- A
- 摘要:
- 主要介绍了通过生物学、血清学、电子显微镜以及分子生物学方法检测植物病毒的原理及特点, 并重点介绍了近几年发展起来的免疫胶体金技术和实时荧光定量PCR 技术。
- Abstract:
- The paper mainly introduced the theo ries and characte ristics o n the me thods to detect plant virus by applying bio logical , se ro logical , elect ron micro scopical and mo lecular techniques , and emphasised o n the Immunogo ld -label assay and real -time f luorescent quanti tative PCR techniques developed in recent y ear s .
参考文献/References:
[ 1] 吴凌娟, 张雅奎, 董传民, 等.用指示植物分离鉴定马铃薯轻花叶病毒(PVX)的技术[ J] .中国马铃薯, 2003 , 17(2):82-83.
[ 2] 周雪平, 濮祖芹.应用斑点法检测植物病毒的研究[ J] .病毒学杂志, 1990 ,(3):317-32.
[ 3] 焦奎, 张书圣.伏安酶联免疫分析法及其在植物血清学检测技术中的应用[ J] .化学通报, 2000 ,(10):50-55.
[ 4] 张建军, 莫晓凤.一种快速简便的植物病毒检测方法[ J] .植物检疫, 1998 , 12(4):222-223.
[ 5] 张建军, 谢为龙.兰花病毒病研究进展[ J] .植物检疫, 1999 , 13 (2):109-111.
[ 6] Ab dul S am ad N , Ari Z .The u se of antib ody -sen siti zed lat ex t o det ect Cymbidium m osai c viru s in orchid s[ J] .Pert emika Jou rnal of Tropical Agricu lture Sci ence , 1993 , 16(2):157-160.
[ 7] 徐平东.免疫胶体金技术及其在植物病毒研究中的应用[ J] .植物检疫, 1993 , 7(4):327-328.
[ 8] 傅仓生, 秦臻.胶体金免疫电镜技术用于烟草花叶病毒的检测 [ J].植物病理学报, 1994 , 24(4):353-355.
[ 9] 魏梅生.斑点免疫金和免疫金/ 银染色法检测烟草环斑病毒[ J] . 植物检疫, 2000 , 14(1):1-2.
[ 10] 洪健, 陈集双, 周雪平, 等.植物病毒的电镜诊断[ J] .电子显微学报, 1999 , 18(3):274-289.
[ 11] Derri ck K S .Qaunt it at ive as say f or plant viruses using serol ogically s pecif ic elect ron microscopy[ J] .Vi rology , 1973 , 56 :652.
[ 12] Milne R G .Elect ron micros copy for the identif icat ion of plant vi ru ses in vit ro preparat ions [ J] .Meth od s in Virology , 1984 , (4):87-120.
[ 13] Kat z D , Kohn A .Imm unosorbent elect ron mi croscopy f or det ecti on of vi ruses[ J] .Advances in Vi ru s Research , 1984 , 29 :169- 194.
[ 14] S hukla D D, Gough K V .The u se of protein A f rom S taphyl ococc usaureu s in immu ne elect ron micros copy f or detect ing plant vi ru s part icles[ J] .Gen .Virol., 1979 , 45 :533-536.
[ 15] Lin N S .Gold -IgG complexes imp rove the detect ion and ident ifi cati on of viru ses in leaf dip preparati ons[ J] .Virol.Methods , 1984 ,(8):181-190.
[ 16] Lou ro D , Lesem an n D-E .Use of prot ein A -gold com plex f or specifi c labeling of an tibodies b ou nd t o plant vi ru ses .1 .Vi ral ant igens in suspension s[ J] .Virol.M ethods , 1984 ,(9):107-122.
[ 17] 孟清, 曹先维, 张彤.应用Digoxigenin 标记的cDNA 探针检测香蕉束顶病毒[ J] .内蒙古大学学报(自然科学版), 1999 , 30(3): 367-371.
[ 18] 田文会, 王江柱.双链检测技术在鉴定病毒中的应用[ J].河北农业大学学报, 1995 , 18(4):112-117.
[ 19] 周雪平, 李德葆.双链检测技术在植物病毒研究中的应用[ J] . 生物技术, 1995 , 5(1):1-4.
[ 20] M orri s T J , Dodds J A.Isolation and analy sis of dou ble st randed RNA f rom virus inf ected plant and fungal ti ssue[ J] .Phyt opathology , 1979 , 67 :854-858.
[ 21] 黄培堂.PCR 技术试验指南[ M] .北京:科学出版社, 1998 .
[ 22] 王继华, 瞿素萍, 孔宝华, 等.百合无症病毒的RT -PCR 和IC -RT -PCR 检测[ J] .云南农业大学学报, 2004 , 19(2):148- 150.
[ 23] Deb orab S G .Quanti tat ive real - time polymerase chain react ion for the core f acilit y using T aqMan and the Perkin - Elmer- PApplied bi osyst em s division 7700 sequence det ect or[ J ] .Jou ranl of Bi om ol ecu lar Techniques , 1999 , 10(1):11-16.
[ 24] 朱建裕, 朱水芳, 廖晓兰, 等.实时荧光RT -PCR 一步法检测番茄环斑病毒[ J] .植物病理学报, 2003 , 33(4):338-341.
相似文献/References:
[1]刘路,申流柱,吴俊,等.毕节市无公害蔬菜农药残留检测分析[J].黑龙江农业科学,2014,(04):66.
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(03):66.
[2]杨洪一,张娜娜,郭世辉,等.植物病毒种传机制研究进展[J].黑龙江农业科学,2012,(06):150.
YANG Hong-yi,ZHANG Na-na,GUO Shi-hui,et al.Advances in Seed-borne Mechanism of Plant Virus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(03):150.
[3]何晓青,程 莉,朱 轶,等.饮用水中病原微生物检测方法与评价标准[J].黑龙江农业科学,2010,(07):111.
HE Xiao-qing,CHENG Li,ZHU Yi,et al.Progress in Monitoring Approaches and Criteria for the Waterborne Pathogens in Drinking Water[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):111.
[4]田方方,王晓萍.除草剂使它隆残留的分析方法简述[J].黑龙江农业科学,2010,(11):65.
TIAN Fang-fang,WANG Xiao-ping.Analysis Methods on the Residues ofthe Herbicide Starane[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(03):65.
[5]胡珅,程阳,栾凤侠.转基因作物及产品检测技术的研究进展[J].黑龙江农业科学,2013,(11):135.
HU Shen,CHENG Yang,LUAN Feng-xia.Research Progress of Detection Methods for Genetically Modified Crops and Products[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(03):135.
[6]陈君,蔡富尧,蔡冲.咪鲜胺乳油在温州蜜柑果实中的动态变化[J].黑龙江农业科学,2015,(01):121.[doi:10.11942/j.issn1002-2767.2015.01.0121]
CHEN Jun,CAI Fu yao,CAI Chong.Dynamic Change of Prochloraz EC in Citrus unshiu Fruit[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(03):121.[doi:10.11942/j.issn1002-2767.2015.01.0121]
[7]. 参与植物病毒移动的蛋白质[J].黑龙江农业科学,1994,(06):25.[doi:10.11942/j.issn1002-2767.1994.06.0025]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,1994,(03):25.[doi:10.11942/j.issn1002-2767.1994.06.0025]
[8]申 宇,高艳玲,范国权,等.DAS-ELISA与RT-PCR法检测马铃薯S病毒的比较[J].黑龙江农业科学,2016,(10):58.[doi:10.11942/j.issn1002-2767.2016.10.0058]
SHEN Yu,GAO Yan-ling,FAN Guo-quan,et al.Comparison of DAS-ELISA and RT-PCR Method for Detection of Potato Virus S[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):58.[doi:10.11942/j.issn1002-2767.2016.10.0058]
[9]隋娟娟,刘佩冉,宁伟,等.太和香椿茎、叶、花中总黄酮含量的比较[J].黑龙江农业科学,2016,(12):92.[doi:10.11942/j.issn10022767.2016.12.0092]
SUI Juanjuan,LIU Peiran,NING Wei,et al.Comparison on Total Flavonoids Content in Stem,Leaf and Flower of Toona sinensis in Taihe County[J].HEILONGJIANG AGRICULTURAL SCIENCES,2016,(03):92.[doi:10.11942/j.issn10022767.2016.12.0092]
备注/Memo
收稿日期:2005 -03 -03
第一作者简介:金羽(1979 -), 女, 黑龙江省铁力县人, 在读硕士, 从事植物病毒的检测与鉴定研究。