[1]孟昭河,孟巧霞,刘永巍,等.根癌农杆菌介导反义蜡质基因获得水稻植株研究初报[J].黑龙江农业科学,2005,(02):8-11.[doi:10.11942/j.issn1002-2767.2005.02.0008]
　MENG Zhao-he,MENG Qiao-xia,LIU Yong-wei,et al.Primary Studies on Transformation of Waxy Antisense Gene Mediated by Agrobacterium in Japonica Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2005,(02):8-11.[doi:10.11942/j.issn1002-2767.2005.02.0008]
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根癌农杆菌介导反义蜡质基因获得水稻植株研究初报

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2005年02 页码: 8-11 栏目: 出版日期: 2005-03-10
Title:
Primary Studies on Transformation of Waxy Antisense Gene Mediated by Agrobacterium in Japonica Rice
文章编号:
1002 - 2767(2005)02 - 0008 - 04
作者:
孟昭河;  孟巧霞;  刘永巍;  李春光;  张景龙
黑龙江省农垦科学院水稻研究所, 佳木斯154025
Author(s):
MENG Zhao-he ;  MENG Qiao-xia ;  LIU Yong-wei ;  LI Chun-guang ;  ZHANG Jing-long
Rice Research Institute of Land Reclamation Academy o f Heilo ng jiang Province , Jiamusi 154025
关键词:
反义蜡质基因; 遗传转化; 根癌农杆菌; 水稻
Keywords:
waxy antisanse gene ; ransfo rmatio n ; ag ro bacterium tumefacien ; rice
分类号:
S511. 035. 3
DOI:
10.11942/j.issn1002-2767.2005.02.0008
文献标志码:
A
摘要:
采用根癌农杆菌介导法, 将水稻反义蜡质基因导入寒地4 个水稻品种(系)成熟胚的愈伤组织。这些愈伤组织经连续2 次25 ～ 50 mg /L 潮霉素抗性筛选, 获得抗性愈伤组织的转化频率为 12. 15 %～ 25. 95 %, 经分化培养, 不同品种的绿苗转化频率分别为2. 49 %～ 4. 43 %。分化成苗的植株经G US 检测, 80 %以上的植株叶片呈阳性反应, 显示兰色。经PCR 检测, 也能扩增出外源基因相应的DNA 片断, 证明外源基因已整合到转基因植株中。
Abstract:
Waxy anti sense gene w as transfe rred into calli derived from immature embryo s of four rice va rieties by Ag robacterium tumefaciens. These calli we re cul tived o n selective media co ntaining 25 ～ 50 mg /L Hyg romy cin tw o times , 12. 15 %～ 25. 95 % resi stant calli . These resistant calli were t ransferred to dif ferentiatio n and regeneration media , 2. 49 % ～ 4. 43 % calli g row n up to plant s. A ssay of Gus activi ty showed that more than 80 % o f t ransgenic plants are Gus posit ive. A few t ransgenic plants w hich w ere negat ive for the Gus assay were analysed by PCR , foreign DNA fragment s w ere detected in these plants. The re sults show ed that fo reig n genes have been inse rted into rice plants.

参考文献/References:

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备注/Memo

收稿日期:2004 - 11 - 22 　
本试验得到了上海农科院作物育种栽培研究所沈革志研究员、殷丽青副研究员和中科院上海植物生理研究所王宗阳研究员的大力支持和指导, 在此表示感谢　
基金项目:国家“ 863” 和黑龙江省农垦总局项目　
第一作者简介:孟昭河(1966 -), 男, 黑龙江省勃利县人, 副研究员, 从事植物生物技术育种研究。

更新日期/Last Update: 2016-12-07