MA Fengjie,YANG Chunyin,SU Cuicui,et al.Effects of Interaction Between Vermicompost and Microbial Agents on Growth of Honey Dew Melon and Soil Nutrients[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(09):25-34.[doi:10.11942/j.issn1002-2767.2023.09.0025]
蚯蚓粪和微生物菌剂互作对蜜瓜生长和土壤养分的影响
- Title:
- Effects of Interaction Between Vermicompost and Microbial Agents on Growth of Honey Dew Melon and Soil Nutrients
- 文章编号:
- 5
- Keywords:
- vermicompost; microbial agents; honey dew melon; quality; soil enzyme activities
- 文献标志码:
- A
- 摘要:
- 为了缓解民勤蜜瓜种植上存在的连作障碍问题，以民勤蜜瓜银蒂为试验材料，采用大田试验，选用新型改良材料蚯蚓粪、根际促生菌、EM微生物菌剂，设置5种施肥处理，分别为OPE、OP、OE、O、CK，测定蜜瓜生长指标、产量、营养品质、土壤养分和酶活性，探讨蚯蚓粪有机肥和微生物菌剂配施在民勤蜜瓜栽培中的应用效果。结果表明，蚯蚓粪和微生物菌剂配施能够增加蜜瓜株高、茎粗、最大叶面积；蚯蚓粪和微生物菌剂配施显著提高土壤微生物活性，增加土壤酶活性，脲酶、过氧化氢酶、碱性磷酸酶表现为成熟期酶活性较开花期酶活性高；多酚氧化酶和硝酸还原酶活性则是开花期酶活性高于成熟期；蚯蚓粪和微生物菌剂配施显著提高蜜瓜土壤有机质含量（4.45%~25.62%），微生物碳、微生物氮含量均呈现增加的趋势，表现为OPE和OP处理含量最高，对其余养分的影响较小；蚯蚓粪和微生物菌剂配施能够显著提高蜜瓜维生素C含量、可溶性蛋白含量和可溶性糖含量，降低蜜瓜有机酸含量和硝酸盐含量，产量较对照增加了8.93%~29.59%。利用隶属函数综合评价分析各处理间改良效果，综合评价指数排序OPE处理得分最高，其次是OP、OE、O和CK，蚯蚓粪和两种微生物菌剂配施效果最好，蚯蚓粪和单一菌剂配施效果次之，单施蚯蚓粪效果较差，根际促生菌较EM微生物菌剂效果好。
- Abstract:
- In order to alleviate the continuous cropping obstacles in Minqin honey dew melon cultivation， this study explored the application effect of earthworm manure organic fertilizer and microbial inoculants in Minqin honey dew melon cultivation. In this experiment， Minqin honey dew Yindi was used as the experimental material， and a field experiment was conducted. New improved materials such as earthworm dung， rhizosphere growth promoting bacteria， and EM microbial inoculants were selected. Five fertilization treatments， OPE， OP， OE， O， and CK， were set up to determine the growth index， yield， nutrient quality， soil nutrients， and enzyme activity of honeydew. The results showed that the combination of earthworm manure and microbial inoculants could increase the plant height， stem diameter， and maximum leaf area of honey dew melon; The combination of earthworm manure and microbial inoculants significantly increased soil microbial activity and soil enzyme activity. Urease， catalase， and alkaline phosphatase showed higher enzyme activity in mature stage than in flowering stage; The activities of polyphenol oxidase and nitrate reductase in flowering stage were higher than those in mature stage; The combination of earthworm manure and microbial inoculants significantly increased the organic matter content of honeydew soil (4.45%-25.62%)， and the microbial carbon and microbial nitrogen content showed an increasing trend， with the highest content in T1 and T2 treatments， with a small impact on other nutrients; The combination of earthworm manure and microbial inoculants can significantly increase the vitamin C content， soluble protein， and soluble sugar content of honey dew melon， reduce the organic acid content and nitrate content of honeydew melon， and increase the yield per mu by 8.93% to 29.59% compared to the control. The comprehensive evaluation and analysis of the improvement effect among various treatments using the membership function showed that the comprehensive evaluation index ranked OPE treatment with the highest score， followed by OP， OE， O， and CK， The effect of earthworm manure combined with two microbial agents was the best， followed by earthworm manure combined with a single microbial agent. The effect of earthworm manure alone was poor， and the effect of rhizosphere growth promoting bacteria was better than that of EM microbial agent.
参考文献/References:
［1］周自政，多峰.甘肃民勤露地蜜瓜高效栽培技术[J].果树实用技术与信息，2021(9):20-22.[2]赵文玉，李海荣，雷虎.瓜州县广至藏族乡设施蜜瓜产业发展现状及对策[J].农业科技与信息，2021(12):55-57.[3]民勤县融媒体中心.甘肃民勤：优品种拓销路 蜜瓜产业“精准”升级“强引擎”[EB/OL].(2023-07-12)[2023-07-15].https://new.qq.com/rain/a/20230712A06VFP00.[4]孙喜军，吕爽，高莹，等.蚯蚓粪对作物连作障碍抑制作用研究进展[J].土壤，2020，52(4):676-684.[5]刘学才.蚯蚓粪对番茄根区环境及产量和品质的影响[D].泰安：山东农业大学，2020.[6]李婉茹，郭延亮，王恩煜，等.优化施肥配施蚯蚓粪有机肥对薄皮甜瓜产量和品质的影响[J].中国瓜菜，2021，34(10):67-73.[7]王丙磊，王冲，刘萌丽.蚯蚓对土壤-植物系统生态修复作用研究进展[J].应用生态学报，2021，32(6):2259-2266.[8]熊孝楠.蚓粪用量对设施内番茄连作土壤酶活性的调节[D].沈阳：沈阳农业大学，2016.[9]曹旭，张先成，王向向，等.蚯蚓粪对番茄根际土壤微生物学特性的影响[J].北方园艺，2021(15):97-105.[10]胡艳霞，孙振钧，孙永明，等.蚯蚓粪对黄瓜炭疽病的系统诱导抗性作用[J].应用生态学报，2004(8):1358-1362.[11]胡艳霞，孙振钧，周法永，等.蚯蚓粪对黄瓜苗期土传病害的抑制作用[J].生态学报，2002(7):1106-1115.[12]戚嘉琦.高温闷棚配施生物制剂对草莓连作障碍修复作用的研究[D].淮安：淮阴工学院，2021.[13]马二磊，黄芸萍，臧全宇，等.4种微生物菌剂对多年连作甜瓜土壤理化性质的影响[J].浙江农业科学，2021，62(6):1129-1132.[14]马二磊，黄芸萍，臧全宇，等.4种微生物菌剂对多年连作甜瓜土壤真菌群落的影响[J].中国瓜菜，2021，34(4):15-20.[15]关松荫．土壤酶及其研究法［Ｍ］．北京：农业出版社，1986.[16]曹建康，姜微波，赵玉梅．果蔬采后生理生化实验指导［M］．北京：中国轻工业出版社，2007．[17]KMEOV M，KOVCc3.TIF;S-*8;Z-*7;Y-*8,IK P.The impact of vermicompost application on yield parameters of maize(Zea mays L.) observed in selected phenological growth stages (BBCH-scale)[J].Acta Fytotechnica et Zootechnica，2014，17(4)：100-108.[18]ARANCON N Q，EDWARDS C A，BIERMAN P，et al.Effects of vermicomposts produced from cattle manure，food waste and paper waste on the growth and yield of peppers in the field [J].Pedobiologia，2005，49(4)：297-306.[19]ARANCON N Q，EDWARDS C A，LEE S S，et al.Management of plant parasitic nematode populations by use of vermicomposts [C]//Brighton Crop Protection Conference Pests and Diseases.2002:705-716.[20]FERNNDEZ-LUQUEO F，REYES-VARELA V，MAR-TNEZ-SUREZ C，et al.Effect of different nitrogen sources on plant characteristics and yield of common bean (Phaseolus vulgaris L.) [J].Bioresource Technology，2010，101(1):396-403.[21]BONANOMI G，ANTIGNANI V，CAPODILUPO M.et al.Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases[J].Soil Biology and Biochemistry，2010，42(2):136-144.[22]赵海涛，赵雷明，姚旭，等.添加蚓粪和蛭石对沟塘底泥育苗基质培育番茄幼苗的影响[J].中国土壤与肥料，2015(3):88-94.[23]韩顺斌，马丽君，华军，等.蚯蚓粪和化肥配施对日光温室番茄产量和品质的影响[J].农业科技与信息，2020(13):19-21.[24]WARDLE 〖KG(0.2mm〗D A.Communities and ecosystems：linking 〖KG)〗the aboveground and belowground components(MPB-34)[M].Princeton University Press,2013.[25]黄自光.专用肥配施纳米蚯蚓粪生物有机肥对苹果、番茄产量与品质的影响[D].杨凌：西北农林科技大学，2021.[26]JINDO K，CHOCANO C，de AGUILAR J M，et al.Impact of compost application during 5 years on crop production，soil microbial activity，carbon fraction，and humification process［J］.Communications in Soil Science and Plant Analysis，2016,47：1907-1919.[27]CATANZARO C J,WILLIAMS K A,SAUVE R J.Slow release versus water soluble fertilization affects nutrient leaching and growth of potted chrysanthemum[J].Journal of Plant Nutrition，1998，21(5)：1025-1036.[28]熊孝楠.蚓粪用量对设施内番茄连作土壤酶活性的调节[D].沈阳：沈阳农业大学，2016.[29]KUMAR V，SINGH K.Enriching vermicompost by nitrogen fixing and phosphate solubilizing bacteria[J].Bioresource Technology，2001,76(2):173-175.[30]赵凤艳.蚓粪改善设施番茄连作土壤质量和番茄品质的机理[D].沈阳：沈阳农业大学，2018.[31]BANDICK A K,DICKA R P.Field management effects on soil enzyme activities[J].Soil Biology and Biochemistry，1999，31(11):1471-1479.[32]TOSCANO G，COLARIETI M L，GRECO J G.Oxidative polymerisation of phenols by a phenol oxidase from green olives[J].Enzyme and Microbial Technology，2003，33(1):47-54.
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备注/Memo
收稿日期：2023-06-16