LIN Jiayu,LI Jinhong,FENG Xiangchi,et al.Effects of Gene on Fertility in Arabidopsis thaliana[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(08):1-7.[doi:10.11942/j.issn1002-2767.2023.08.0001]
基因对拟南芥育性的影响
- Title:
- Effects of Gene on Fertility in Arabidopsis thaliana
- 文章编号:
- 1
- 关键词:
- 拟南芥; 成束状阿拉伯半乳糖蛋白; 雄性育性
- 文献标志码:
- A
- 摘要:
- 成束状阿拉伯半乳糖蛋白（Fasciclin Like Arabinogalactan Proteins，FLAs）广泛存在于植物中，参与调控植物多个生长发育阶段。相关研究的转录组数据表明AtFLA22在拟南芥绒毡层中特异表达，推测其功能可能与花粉发育有关。为探究AtFLA22功能，通过eFP Browser数据库基因组织表达数据绘制热图，并通过qRTPCR实验进一步确认AtFLA22在花中特异表达，推测AtFLA22参与雄性育性调控。为进一步探究AtFLA22基因功能，利用CRISPR/Cas9技术创制了atfla22基因敲除突变体，其目标基因第108位碱基插入腺嘌呤A可能导致了蛋白翻译提前终止，对育性表型进行观察，发现该突变体果荚变短，种子出现败育表型。因此，从遗传上揭示AtFLA22参与了拟南芥雄性育性调控。
- Abstract:
- Fasciclin-like arabinogalactan proteins (FLAs) are widely distributed in various plants and are involved in plant growth and development. was identified through RNA-seq from previous study of other group， and its function may be related to anther and pollen development. In order to explore the function of ， heat map was drawn according to the data from eFP Browser database， and meanwhile qRT-PCR experiments were performed and further confirmed that was specific expressed in the flowers. Combined with the above data， it was speculated that may be involved in fertility regulation. In addition， atfla22 mutant was generated by CRISPR/Cas9 and the insertion of adenine at 108 bp may lead to premature termination of protein translation， and lead to the phenotype with shorter pods and abortive seeds. In conclusion， we revealed that functions in the regulation of male fertility of Arabidopsis thaliana.
参考文献/References:
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[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):163.[doi:10.11942/j.issn1002-2767.2019.06.0163]
备注/Memo
收稿日期：2023-04-04