ZHOU Li,ZHANG Shen,CHEN Lin,et al.Screening of Highly Virulent Strains of Spodoptera litura and Virulence Determination of Four Insecticides[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(04):44-50.[doi:10.11942/j.issn1002-2767.2022.04.0044]
斜纹夜蛾高致病力菌株的筛选及四种杀虫剂的毒力测定
- Title:
- Screening of Highly Virulent Strains of Spodoptera litura and Virulence Determination of Four Insecticides
- Keywords:
- Spodoptera litura; entomopathogenic fungi; insecticides; virulence detection; biological control
- 文献标志码:
- A
- 摘要:
- 为筛选出相容性好且具有增效作用的菌药复配剂，本研究通过饲喂法，选用永州地区土壤中分离纯化的66株菌株以及保藏于华南农业大学生物防治中心的13株菌株，对斜纹夜蛾二龄幼虫进行高毒力菌株筛选试验，筛选出的高毒力菌株以不同浓度梯度进行毒力测定。同时测定了不同浓度梯度甲维盐、阿维菌素、多杀霉素和氯虫苯甲酰胺对斜纹夜蛾二龄幼虫的致死率。结果表明：孢子悬浮液浓度为1×108 conidia·mL-1时，79株菌株处理斜纹夜蛾二龄幼虫7 d后，球孢白僵菌SCAUYZ16和SCAUJH19的致病力最高，其校正死亡率分别为70.71%和57.84%，LT50分别为5.01和5.98 d。球孢白僵菌SCAUYZ16、SCAUJH19以1×104，1×105，1×106，1×107和1×108 conidia·mL-1的浓度梯度进行毒力分析试验，LC50分别为3.29×107和8.09×107 conidia·mL-1。甲维盐、阿维菌素、多杀霉素和氯虫苯甲酰胺分别对斜纹夜蛾二龄幼虫处理3 d后，LC50分别为3.56，59.14，18.82和2.76 mg·L-1。SCAUYZ16和SCAUJH19对斜纹夜蛾有较高的毒力，可作为斜纹夜蛾生物防治的潜力菌株。在后续试验中，可选取杀虫剂较低的LC50剂量为基础，设置不同浓度梯度进行菌药复配试验。
- Abstract:
- In order to screen out the bacterial-drug blends with good compatibility and synergistic effect,through the feeding method,66 strains isolated and purified from the soil in the Yongzhou area,as well as 13 strains preserved at the Biological Control Center of South China Agricultural University,were used to screen the highly virulent strains for the second instar larvae of Spodoptera litura,the highly virulent strains were assessed using different concentration gradients.At the same time,the mortality of emamectin benzoate,abamectin,spinosad,and chlorantraniliprole against the second instar larvae of insects was studied using different concentration gradients.When the spore suspension concentration of 79 strains was 1×108 conidia·mL-1 and the second instar larvae of Spodoptera litura were treated for 7 days,the results showed that Beauveria bassiana SCAUYZ16 and SCAUJH19 had the highest virulence,with corrected mortality rates of 70.71% and 57.84%,LT50 of 5.01 days and 5.98 days,respectively.The virulence of Beauveria bassiana SCAUYZ16 and SCAUJH19 were tested at concentration gradients of 1×104,1×105,1×106,1×107,and 1×108 conidia·mL-1,with LC50 values of 3.29×107 and 8.09×107 conidia·mL-1,respectively.After treatment with emamectin benzoate,abamectin,spinosad and chlorantraniliprole on the second instar larvae of Spodoptera litura for 3 days,and the LC50 were 3.56,59.14,18.82 and 2.76 mg〖DK1〗·L-1.SCAUYZ16 and SCAUJH19 showed a high level of virulence against Spodoptera litura and could be exploited as prospective Spodoptera litura biological control strains.The lower LC50 dose of the pesticide can be used as the basis for the follow-up test,and different concentration gradients for the compound test of bacteria and pharmaceuticals can be adjusted.
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备注/Memo
收稿日期：2021-12-24
基金项目：湖南省烟草公司永州市公司2018年度烟叶科研项目计划（永烟技[2018]45号）。
第一作者：周立（1995-），男，硕士，助理农艺师，从事农业昆虫与害虫防治研究。E-mail：869305801@qq.com。
通信作者：李佳颖（1985-），女，硕士，农艺师，从事农业昆虫与害虫防治研究。E-mail：jiaying-li217@139.com。