[1]刘 悦,王立达,兰 英,等.Hi-Ⅱ玉米的NGc抗虫基因遗传转化研究[J].黑龙江农业科学,2022,(01):1-5.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0001]
　LIU Yue,WANG Li-da,LAN Ying,et al.Study on Genetic Transformation of NGc Insect Resistant Gene in HI-Ⅱ Maize Immature Embryo[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(01):1-5.[doi:DOI:10.11942/j.issn1002-2767.2022.01.0001]
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Hi-Ⅱ玉米的NGc抗虫基因遗传转化研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年01 页码: 1-5 栏目: 出版日期: 2022-01-10
Title:
Study on Genetic Transformation of NGc Insect Resistant Gene in HI-Ⅱ Maize Immature Embryo
作者:
刘 悦; 王立达; 兰 英; 李青超; 刘 洋; 郑 旭; 赵秀梅
黑龙江省农业科学院 齐齐哈尔分院，黑龙江 齐齐哈尔 161006
Author(s):
LIU Yue; WANG Li-da; LAN Ying; LI Qing-chao; LIU Yang; ZHNEG Xu; ZHAO Xiu-mei
Qiqihar Branch,Heilongjiang Academy of Agricultural Sciences,Qiqihar 161006,China
关键词:
玉米; 抗虫基因CryNGc; 遗传转化; PCR
Keywords:
maize;  insect resistant gene CryNGc;  genetic transformation;  PCR
DOI:
DOI:10.11942/j.issn1002-2767.2022.01.0001
文献标志码:
A
Abstract:
In order to promote the research of transgenic insect resistant maize,in this study,the target gene were transferred into maize material Hi-Ⅱby Agrobacterium-mediated genetic transformation of maize embryos,maize plants with CryNGc anti-insect genes were cultured by explant infection,co-culture and differentiation screening,and the genetic expression and resistance of exogenous genes in the offspring were studied.The results showed that at the 1.2-1.8 mm maize embryosize,the infection effect was the best,and ten positive transformed plants with CryNGc insect resistance genes were successfully obtained,and the transformation efficiency was 1.428‰.

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备注/Memo

收稿日期：2021-10-28

基金项目：中国科学院战略性先导科技专项(XDA28130504);黑龙江省农业科学院“农业科技创新跨越工程”专项（HNK2019CX14）。

第一作者：刘悦(1995-)，女，硕士，研究实习员，从事植物保护研究。E-mail：2563522180@qq.com。

更新日期/Last Update: 2022-04-01