[1]杜翔鹰,谢颖苑,廖飞扬,等.木麻黄外植体选择与启动研究[J].黑龙江农业科学,2020,(01):104-106.[doi:10.11942/j.issn1002-2767.2020.01.0104]
　DU Xiang-ying,XIE Ying-yuan,LIAO Fei-yang,et al.Study on the Selection and Initiation of Casuarina equisetifolia Explants[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(01):104-106.[doi:10.11942/j.issn1002-2767.2020.01.0104]
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木麻黄外植体选择与启动研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2020年01期 页码: 104-106 栏目: 出版日期: 2020-01-10
Title:
Study on the Selection and Initiation of Casuarina equisetifolia Explants
作者:
杜翔鹰; 谢颖苑; 廖飞扬; 黄良宙
湛江市林业科学研究所，广东 湛江 524037
Author(s):
DU Xiang-ying; XIE Ying-yuan; LIAO Fei-yang; HUANG Liang-zhou
Zhanjiang Forestry Science Research Institute,Zhanjiang 524037,China
关键词:
木麻黄; 外植体; 灭菌; 防褐化; 组织培养
Keywords:
Casuarina equisetifolia;  explants;  sterilization;  anti-browning;  tissue culture
DOI:
10.11942/j.issn1002-2767.2020.01.0104
文献标志码:
A
摘要:
为探索木麻黄优良无性系品种抗性退化问题，对木麻黄优良无性系外植体启动进行组织培养试验，通过9组方案进行组合试验。结果表明：木麻黄以木质化外植体，选择用70%乙醇+0.3%新洁尔灭+3%次氯酸钠+0.5%升汞的灭菌方案，能达到最佳的少毒杀、灭菌、少褐化、高成活率等的效果。
Abstract:
In order to explore the problem of resistance degradation of superior clones of Casuarina equisetifolia,the tissue culture experiment was carried out on the explants of superior clones of Casuarina equisetifolia through 9 groups of schemes.The results showed that the best sterilization scheme was 70% ethanol + 0.3%Bromogeramine+3%sodium hypochlorite+0.5% mercuric chloride,which could achieve the effects of less toxicity,sterilization,less browning and high survival rate.

参考文献/References:

［1］李莹,罗晓芳,蒋湘宁.美国红枫外植体选择及启动培养研究[J].黑龙江农业科学,2010(8):6-9.

[2]刘英,仲崇禄,白嘉雨,等.不同盐浓度对木麻黄无性系愈伤组织的影响[J].广东林业科技,2003,19(2):47-50.

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备注/Memo

收稿日期：2019-08-01第一作者：杜翔鹰（1986-），男，林业工程师，从事植物组织培养研究。E-mail：dutstudent@126.com。

更新日期/Last Update: 2020-04-23