LI Liang-liang,LI Wei-jie,WU Di,et al.Optimization of the Rapid Propagation System of Hongyang Kiwifruit[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(09):17-21.[doi:10.11942/j.issn1002-2767.2019.09.0017]
红阳猕猴桃快繁体系优化
- Title:
- Optimization of the Rapid Propagation System of Hongyang Kiwifruit
- Keywords:
- Hongyang kiwifruit; callus; adventitious bud; rooting medium
- 文献标志码:
- A
- 摘要:
- 为提高红阳猕猴桃组织培养效率，以红阳猕猴桃雌株幼嫩的不带芽枝条、带叶脉和不带叶脉叶片为外植体，通过不同激素（6-BA、NAA）不同浓度诱导外植体出不定芽、生根等处理，建立高效稳定的快速繁殖体系。结果表明：幼嫩的不带芽茎段和带叶脉的叶片极易形成愈伤组织和不定芽，经过筛选最适诱导不定芽的培养基分别为MS+3 mg·L-1 -6-BA+0.2 mg·L-1- NAA和MS+2 mg·L-1- 6-BA+0.3 mg·L-1- NAA，生长大量的愈伤组织之后萌发不定芽，出芽率均能达到100%，成芽数/接种数分别达到4.6和4.4。采用不带芽的幼嫩茎段为外植体时，减少了试验材料的浪费，两种培养基均能不断再生不定芽，省去了增殖培养基的筛选，节省了培育时间。1/2 MS+0.7 mg·L-1- IBA诱导不定芽生根效果最佳，生根率达到90%。
- Abstract:
- In order to improve the tissue culture efficiency of kiwifruit,the young non-budding branches,leaves with leaf veins and leaves without leaf veins of Hongyang kiwifruit were used as explants to establish an efficient and stable rapid propagation system by inducing adventitious buds and rooting of explants with different hormones (6-BA,NAA) at different concentrations.The results showed that young leaves without bud stems and leaves with veins were easy to form callus and adventitious buds.The optimal medium for inducing adventitious buds was MS+3 mg· L-1- 6-BA+0.2 mg·L-1- NAA and MS+2 mg·L-1- 6-BA+0.3 mg·L-1- NAA.The adventitious buds were germinated after growing a large amount of callus in the medium,and the germination rate reached 100%,and the number of germination/inoculation reached 4.6 and 4.4.In this study,young stems without buds were used as explants,which reduced the waste of test materials.Both media were able to continuously regenerate adventitious buds,eliminating the need for screening of value-added media and saving cultivation time.1/2 MS+0.7 mg·L-1- IBA induced the best rooting effect of adventitious buds,and the rooting rate reached 90%.
参考文献/References:
［1］-徐小彪，张秋明．中国猕猴桃种质资源的研究与利用[J]．植物学通报，2003(6)：648-655．
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备注/Memo
收稿日期：2019-03-28