[1]成妍,乔宁,梁燕平,等.辣椒小孢子胚胎发生早期的组织化学染色[J].黑龙江农业科学,2019,(08):18-21.[doi:10.11942/j.issn1002-2767.2019.08.0018]
　CHENG Yan,QIAO Ning,LIANG Yan-ping,et al.Histochemical Staining of Early Microspore Embryogenesis in Pepper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(08):18-21.[doi:10.11942/j.issn1002-2767.2019.08.0018]
点击复制

辣椒小孢子胚胎发生早期的组织化学染色

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2019年08期 页码: 18-21 栏目: 出版日期: 2019-08-10
Title:
Histochemical Staining of Early Microspore Embryogenesis in Pepper
作者:
成妍; 乔宁; 梁燕平; 田如霞; 焦彦生; 苗如意
山西省农业科学院 蔬菜研究所，山西 太原 030031
Author(s):
CHENG Yan; QIAO Ning; LIANG Yan-ping; TIAN Ru-xia; JIAO Yan-sheng; MIAO Ru-yi
Institute of Vegetable Research,Shanxi Academy of Agricultural Sciences，Taiyuan 030031，China
关键词:
辣椒; 小孢子; 胚胎发生; 组织化学染色
Keywords:
pepper;  microspore;  embryogenesis;  histochemical staining
DOI:
10.11942/j.issn1002-2767.2019.08.0018
文献标志码:
A
摘要:
为研究辣椒小孢子胚胎发生机理，以甜椒品种晋椒203为试验材料进行小孢子培养，制作培养0，3，7，15 d的花药石蜡切片，利用番红-固绿、碘-碘化钾和考马斯亮蓝3种染色液分别对小孢子的细胞质、淀粉粒和蛋白质染色。结果表明：高温诱导3 d后，小孢子体积明显膨大，细胞核移向中央。少数小孢子分裂产生大小、结构特征相等的两个细胞，进而增殖形成多细胞结构。起初具有胚性的小孢子内积累了大量淀粉，随着分裂的进行，有些多细胞结构内会沉积较多的淀粉，也有些多细胞结构没有淀粉积累，不被碘-碘化钾染色。具有胚胎发生能力的小孢子中蛋白质含量减少。这些变化必然影响辣椒小孢子胚状体的产生。
Abstract:
In order to study the mechanism of microspore embryogenesis in pepper,taking a sweet pepper variety Jinjiao 203 as the experimental material,paraffin sections of the anthers were made after 0,3,7 and 15 d of microspore culture,respectively.The cytoplasm,starch grains and protein of microspore was stained with sarranine-fast green,iodine-potassium iodide and Coomassie brilliant blue,respectively.The results showed that the volume of the micropore increased obviously after culturing 3 days and the nuclear migrated to the cente of the micropore.A few microspore divised into two cells which had equal size and structure,and then proliferated into a multicellular structure.A large amount of starch was accumulated in the embryogenetic microspores originally.As the division proceeding,some multicellular structures contained much starch,while the other multicellular structures accumulated little starch,and would not be stained by iodine-potassium iodide.The protein content of microspores with embryogenic ability was reduced.These changes were bound to affect the production of microspore embryoid in pepper.

参考文献/References:

［1］赵建平，姚祥坦.植物小孢子胚胎发生机理 [J].生物技术通报,2009(6): 7-11.

［2］李婷婷.辣椒花药培养及其胚胎发生机理研究[M].太古：山西农业大学,2014.
［3］李婷婷,马蓉丽,成妍,等.蔬菜作物小孢子胚胎发生机制研究进展[J].北方园艺,2014(3): 174-177.
［4］成妍,马蓉丽,焦彦生.季节因素和供体年龄对辣椒小孢子培养的影响[J].辣椒杂志,2012(3): 20-23.
［5］张菊平.辣椒花药小孢子培养及其胚状体发生机理研究[D].杨凌：西北农林科技大学,2007.
［6］王烨.辣椒游离小孢子培养及其显微观察[D].北京：中国农业科学院,2004.
［7］Yan Cheng,Rongli Ma,Yansheng Jiao,et al.Impact of genotype,plant growth regulatorsand activated and activated charcoal on embryogenesis induction in microspore culture of pepper(Capsicum annuum L.)[J].South African Journal of Botany,2013,88:306-309.
［8］耿华美,付强,郭骏,等.垂花青兰蜜腺的发育解剖学研究[J].植物研究,2012,32(1): 22-27.
〖HJ2.2mm〗［9］Maraschin S F,de Priester W,Spaink H P,et al.Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective[J].Journal of Experimental Botany,2005,56: 1711-1726.〖ＺＫ）〗
［10］〖ＺＫ（＃〗张菊平,巩振辉,张兴志,等.辣椒花药培养胚状体发生的组织学和细胞学研究[J].分子细胞生物学报,2009,42(3-4): 200-210.
［11］Barany I,Fadon B,Risueno M C,et al.Microspore reprogramming to embryogenesis induces changes in cell wall and starch accumulation dynamics associated with proliferation and differentiation events[J].Plant Signaling Behavior,2010,5(4):341-345.
［12］Barany I,Fadon B,Risueno M C,et al.Cell wall components and pection esterification levels as markers of proliferation and differentiation events during pollen development and pollen embryogenesis in Capsicum annum L.[J].Journal of Experimental Botany,2010,61(4):1159-1175.
［13］George L,Narayanswamy S.Haploid Capsicum through experimental androgenesis[J].Protoplasma,1973,78(4):467-470.
［14］Pauls K P,Chan J,Woronuk G,et al.When microspores decide to become embryos-cellular and molecular changes[J].〖JP〗Canadian Journal of Botany,2006,84(4): 668-678.

相似文献/References:

[1]杨 光,张 青,龚 娜,等.辣椒工厂化育苗基质筛选的研究[J].黑龙江农业科学,2014,(06):88.
　YANG Guang,ZHANG Qing,GONG Na,et al.Study on Screening of Optimum Substrate forIndustrial Seedling of Hot Pepper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(08):88.
[2]吴文林,毛艳辉,梁玉霞,等.纳米材料对辣椒种子萌发的作用参数优化[J].黑龙江农业科学,2012,(02):54.
　WU Wen-lin,MAO Yan-hui,LIANG Yu-xia,et al.Optimization of Function Parameters of Nanomaterials on Germination of Pepper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):54.
[3]赵发辉,王文霞.辣椒疫病防治研究[J].黑龙江农业科学,2012,(09):61.
　ZHAO Fa-hui,WANG Wen-xia.Prevention and Control of Phytophthora capsici of Pepper[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):61.
[4]迟莉.黄绿木霉菌及其混剂对辣椒植株及土壤的影响[J].黑龙江农业科学,2013,(07):42.
　CHI Li.Effects of Trichoderma aureoviride and its Agents on Hot Pepper Plants and Soil[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):42.
[5]王海鹏,汪玉萍,马育斌,等.秦王川辣椒膜下滴灌栽培新技术[J].黑龙江农业科学,2010,(08):176.
　WANG Hai-peng,WANG Yu-ping,MA Yu-bin,et al.New Cultivation Techniques of Pepper Drip under the Plastic Film in Qinwangchuan[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(08):176.
[6]谢礼洋,匡华琴,赖钟雄,等.辣椒子叶不定芽的诱导与伸长研究[J].黑龙江农业科学,2013,(10):12.
　XIE Li-yang,KUANG Hua-qin,LAI Zhong-xiong,et al.Study on Adventitious Buds Induction and Elongation of Capsicum annuum L. Cotyledon[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):12.
[7]谢礼洋,匡华琴,赖钟雄,等.辣椒愈伤组织诱导的研究[J].黑龙江农业科学,2013,(11):17.
　XIE Li-yang,KUANG Hua-qin,LAI Zhong-xiong,et al.Study on Callus Induction from in vitro Capsicum annuum L.[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):17.
[8]朱 林,于琴芝,胥宇建,等.辣椒新品种的评比筛选试验[J].黑龙江农业科学,2015,(10):110.[doi:10.11942/j.issn1002-2767.2015.10.0110]
　ZHU Lin,YU Qin-zhi,XU Yu-jian,et al.Appraisal Comparison and Screening Trial of New Pepper Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(08):110.[doi:10.11942/j.issn1002-2767.2015.10.0110]
[9]肖淑芹,陆秀华,刘惕若.黑龙江省辣椒疫病发生情况调查[J].黑龙江农业科学,2002,(05):45.[doi:10.11942/j.issn1002-2767.2002.05.0045]
　XIAO Shu-qin,LU Xiu-hua,LIU Ti-ruo.Investigation on Pepper Blight of Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2002,(08):45.[doi:10.11942/j.issn1002-2767.2002.05.0045]
[10]王金玲,吕长山,于广建,等.辣椒碱的研究进展[J].黑龙江农业科学,2004,(03):36.[doi:10.11942/j.issn1002-2767.2004.03.0036]
　WANGJin-ling,L Chang-shan,YU Guang-jian.Study Advances of Capsaicin[J].HEILONGJIANG AGRICULTURAL SCIENCES,2004,(08):36.[doi:10.11942/j.issn1002-2767.2004.03.0036]

备注/Memo

收稿日期：2019-03-27

基金项目：山西省农业科学院特色农业技术攻关项目（YGG17073）；山西省自然科学基金（201701D121090）；山西省重点研发计划项目（201703D221019-2，01703D211019）；山西省留学归国人员科研资助项目（2016-130）；山西省农业科学院育种工程项目（17yzgc038）；山西省基础条件平台项目（201605D121022，201705D121019-6）。
第一作者简介：成妍（1982-），女，博士，副研究员，从事辣椒遗传育种研究。E-mail：chengyan820620@163.com。
通讯作者：苗如意（1964-），男，硕士，研究员，从事蔬菜遗传育种及栽培研究。E-mail：jyseed@163.com。

更新日期/Last Update: 2019-09-12