[1]侯晓宇,尚宏芹,刘梦迪,等.油用牡丹△9-硬脂酰-ACP脱氢酶（SAD）生物信息学分析[J].黑龙江农业科学,2018,(07):23-29.[doi:10.11942/j.issn1002-2767.2018.07.0023]
　HOU Xiao-yu,SHANG Hong-qin,LIU Meng-di,et al.Bioinformatics Analysis of Δ9-stearoyl-ACP Desaturase(SAD) in Paeonia ostii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2018,(07):23-29.[doi:10.11942/j.issn1002-2767.2018.07.0023]
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油用牡丹△9-硬脂酰-ACP脱氢酶（SAD）生物信息学分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2018年07期 页码: 23-29 栏目: 出版日期: 2018-07-10
Title:
Bioinformatics Analysis of Δ9-stearoyl-ACP Desaturase(SAD) in Paeonia ostii
作者:
侯晓宇; 尚宏芹; 刘梦迪; 郭梦露; 高昌勇
菏泽学院 牡丹学院/生理生化及应用山东省高校重点实验室，山东 菏泽 274015
Author(s):
HOU Xiao-yu; SHANG Hong-qin; LIU Meng-di; GUO Meng-lu; GAO Chang-yong
Paeon College,Heze University/Shandong Universities Key Laboratory of Physiology,Biochemistry and Application,Heze 274015,China
关键词:
油用牡丹; 凤丹; △9-硬脂酰-ACP脱氢酶（SAD）; 生物信息学分析
Keywords:
oil peony;  Paeonia ostii;  Δ9- stearoyl-ACP desaturase(SAD);  bioinformatics analysis
DOI:
10.11942/j.issn1002-2767.2018.07.0023
文献标志码:
A
摘要:
Δ9-硬脂酰-ACP脱氢酶(stearoyl-ACP desaturase,SAD)是控制植物油脂中饱和与不饱和脂肪酸比例的关键酶之一。利用生物信息学在线工具，对油用牡丹—凤丹（Paeonia ostii）Po-SAD的氨基酸序列特征、理化性质、二硫键、磷酸化位点、高级结构进行预测，并利用MEGA6.0构建了不同植物SAD分子进化树。结果表明：Po-SAD蛋白在相对分子量、理论pI与芍药、拟南芥的SAD相近，属于稳定的、可溶性蛋白。二级结构包括α螺旋（40.91%）、延伸串（12.37%）和不规则卷曲（46.72%），Po-SAD含有35个磷酸化位点和6个糖基化位点，亚细胞定位在质体中。进化树分析表明，凤丹Po-SAD与芍药的高度同源且亲缘关系最近。
Abstract:
Δ9- stearoyl-ACP desaturase(SAD) is the key enzyme regulating the ratio of saturated and unsaturated fatty acid in plant.In the present paper,bioinformatics tools were employed to characterize SAD proteins in Paeonia ostii，including physicochemical properties,disulfide,phosphorylation sites,subcellular localization,advanced 3D structure.And molecular phylogenetic tree of SAD from different plant were constructed by MEGA 6.0 software.The results showed that the molecular weight and theoretical pI of Po-SAD were similar to the Arabidopsis thaliana and Paeonia lactiflora SADs,belonging to stable,soluble protein.The secondary structure of Po-SAD protein contained 40.91% α helices,12.37% extended strand,46.72% random coil,and contained 35 phosphorylation sites,6 β-O-glycosylation sites.Prediction of subcellular localization showed that Po-SAD located in chloroplast.Analysis of molecular phylogenetic tree showed that the Po-SAD was highly homologous and close to Paeonia lactiflora.All these results provide a scientific reference for understanding the molecular mechanism underlying lipid biosynthesis and special fatty acid high accumulation in oil peony seeds.

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备注/Memo

收稿日期：2018-03-02基金项目：山东省“十三五”高等学校重点实验室资助项目：生理生化及应用实验室资助项目；山东省自然科学基金资助项目(ZR2015CL008)；菏泽学院博士基金资助项目（XY17BS07）；菏泽学院培育资助项目（XY17PY03）。第一作者简介：侯晓宇（1999-），女，在读学士，从事植物生物技术研究。E-mail:1870841586@qq.com。通讯作者：高昌勇（1976-），男，博士，副教授，从事植物油脂代谢工程、植物生物技术研究。E-mail：sxnydx@126.com。

更新日期/Last Update: 2018-09-13