[1]罗 帅,杨洪一.原位PCR原理及应用[J].黑龙江农业科学,2017,(06):108-111.[doi:10.11942/j.issn1002-2767.2017.06.0108]
　LUO Shuai,YANG Hong-yi.In Situ PCR Principle and Application[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(06):108-111.[doi:10.11942/j.issn1002-2767.2017.06.0108]
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原位PCR原理及应用

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2017年06期 页码: 108-111 栏目: 出版日期: 2017-06-10
Title:
In Situ PCR Principle and Application
文章编号:
1002-2767（2017）06-0108-04
作者:
罗 帅; 杨洪一
东北林业大学 生命科学学院，黑龙江 哈尔滨 150040
Author(s):
LUO Shuai; YANG Hong-yi
College of Life Science，Northeast Forestry University，Harbin，Heilongjiang 150040
关键词:
原位杂交; PCR ; 原位PCR
Keywords:
in situ hybridization; PCR; in situ PCR
分类号:
Q78
DOI:
10.11942/j.issn1002-2767.2017.06.0108
文献标志码:
A
摘要:
原位PCR是一种灵敏度特别高的基因水平上检测技术，该技术通过将原位杂交技术和普通的PCR技术相结合，从而可以检测出组织或细胞中低浓度的特异序列。为促进原位PCR技术的发展完善，简要介绍了原位PCR的基本原理、流程及应用情况。
Abstract:
In situ PCR is a highly sensitive gene level detection technique,the technique combines the in situ hybridization with the common PCR technique to detect low concentrations of specific sequences in tissues or cells.In order to promote the development of in situ PCR technology,the basic principle,process and application of in situ PCR were briefly introduced.

参考文献/References:

[1]Mullis K,Faloona F,Scharf S,et al.Specific enzymatic amplification of DNA in vitro:the polymerase chain reaction［J］.Cold Spring HarborSymp.Quant.Biol.1986,51: 263-273.
[2]Gall J G,Pardue M L.Formation and detection of RNADNA hybrid molecules in cytological preparations[J].Proceedings of the National Academy of Sciences of the United States of America,1969,63(2):378.
[3]Haase Ashley T,Retzel Ernest F,Staskus Katnerine A.Amplification and detection of lentiviral DNA inside cells[J].Proceedings of the National Academy of Sciences of the United State of America,1990,87(13): 4971-4975.
[4]陈林姣.原位PCR技术及其应用[J].生物工程进展,2000,2(5):475-478.
[5]谢辉,章伟文,陈宏,等.原位反转录PCR技术及其应用的研究进展[J].医学理论与实践,2003(4):402-403.
[6]李雪梅，李菲菲.原位RT-PCR技术的应用[J].中国兽医科技,2005,3(2):157-160.
[7]商明清,魏梅生.植物病毒检测新技术研究进展[J].植物检疫,2004,18(4)236-240.
[8]Bagasra O,Hauptman S P,Lischner H W,et al.Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction[J].New England Journal of Medicine,1992,326(21):1385-1391.
[9]Bagasra O,Seshamma T,Oakes J W,et al.Frequency of cells positive for HIV1 sequences assessed by in situ polymerase chain reaction[J].Aids,1993,2(11):7.
[10]赵英,牛建新.梨树组织中的苹果锈果类病毒原位RT-PCR检测[J].分子植物育种,2008,6(4):812-818.
[11]Rojas M R,Jiang H,Salati R,et al.Functional analysis of proteins involved in movement of the monopartite begomovirus,Tomato yellow leaf curl virus[J].Virology,2001,291(1):110-25.
[12]黄广明,张曼夫,乔素兰.应用原位PCR对鸡传染性法氏囊病病毒早期侵染过程的研究[J].病毒学报,2001,17(1):60-64.
[13]Murphy MLP,Rodriguez M,Schudel A A,et al.Localization FMDV RNA in tissue culture infected cells via in situ polymerase chain reaction [J].Journal of Virological Methods,1995,54(2-3):173.
[14]李爽,甘绍伯,彭瑞云,等.原位PCR对弓形虫感染鼠毒力检测效果的观察[J].中国人兽共患病杂志,2000,16(3):41-43.
[15]Yin J,Kaplitt M G,Kwong A D,et al.In situ PCR for in vivo detection of foreign genes transferred into rat brain[J].Brain Research,1998,783(2):347.
[16]李远华,江昌俊,余有本.茶树叶片馄咸烟擒彰富虻脑籔CR研究[J].茶叶科学,2004,24(2):147-150.
[17]Wood M J,Byrnes A P,Kaplitt M G,et al.Specific patterns of defective HSV1 gene transfer in the adult central nervous system: implications for gene targeting[J].Experimental Neurology,1994,130(1):127.
[18]Tokusashi Y,Nishikawa Y,Ogawa K.Differentiation of the normal and mutant rat albumin genes on hepatic tissue sections by in situ PCR[J].Nucleic Acids Research,1995,23(18):3790.
[19]Long A A,Komminoth P,Lee E,et al.Comparison of indirect and direct insitu polymerase chain reaction in cell preparations and tissue sections.Detection of viral DNA,gene rearrangements and chromosomal translocations[J].Histochemistry,1993,99(2):151-162.
[20]Komminoth P,Long A A.Insitu polymerase chain reaction[J].Virchows Archiv B,1993,64(1):67-73.
[21]Long A A,Komminoth P,Lee E,et al.Comparison of indirect and direct insitu polymerase chain reaction in cell preparations and tissue sections.Detection of viral DNA,gene rearrangements and chromosomal translocations[J].Histochemistry and Cell Biology,1993,99(2):151-62.
[22]Embleton M J,Gorochov G,Jones P T,et al.Incell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells[J].Nucleic Acids Research,1992,20(15):3831-7.
[23]Uchiumi T,Kuwashiro R,Miyamoto J,et al.Detection of the leghemoglobin gene on two chromosomes of Phaseolus vulgaris by in situ PCR linked fluorescent in situ hybridization(FISH)[J].Plant & Cell Physiology,1998,39(7):790-794.
[24]Heniford B W,ShumSiu A,Leonberger M,et al.Variation in cellular EGF receptor mRNA expression demonstrated by in situ reverse transcriptase polymerase chain reaction[J].Nucleic Acids Research,1993,21(14):3159-66.
[25]Cohen N S.Intracellular localization of the mRNAs of argininosuccinate synthetase and argininosuccinate lyase around liver mitochondria,visualized by highresolution in situ reverse transcriptionpolymerase chain reaction[J].Journal of Cellular Biochemistry,1996,61(1):81-96.
[26]钟筱波.用荧光原位杂交技术构建高分辨率的DNA物理图谱[J].遗传学报,1997(3):44-48.

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备注/Memo

收稿日期：2017-04-24

基金项目：黑龙江省自然科学基金资助项目(LC2016005)
第一作者简介：罗帅（1992-），男，安徽省阜阳市人，在读硕士，从事微生物学研究。E-mail: 847613797@qq.com。
通讯作者：杨洪一（1978-），男，吉林省九台市人，博士，副教授，从事微生物学研究。E-mail:hyi01@tom.com。

更新日期/Last Update: 2017-08-21