[1]王 爽,李新民,刘春来,等.13株植物病原菌拮抗细菌的筛选[J].黑龙江农业科学,2015,(09):64-68.[doi:1002-2767（2015）09-0064-05]
　WANG Shuang,LI Xin-min,LIU Chun-lai,et al.Screening of Antagonistic Bacterium Against 13 Kinds of Common Plant Pathogenic Fungi[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(09):64-68.[doi:1002-2767（2015）09-0064-05]
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13株植物病原菌拮抗细菌的筛选

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2015年09 页码: 64-68 栏目: 出版日期: 2015-09-10
Title:
Screening of Antagonistic Bacterium Against 13 Kinds of Common Plant Pathogenic Fungi
文章编号:
1002-2767（2015）09-0064-05
作者:
王 爽; 李新民; 刘春来; 杨 帆; 夏吉星; 王克勤; 刘兴龙
黑龙江省农业科学院 植物保护研究所，黑龙江 哈尔滨 150086
Author(s):
WANG Shuang; LI Xin-min; LIU Chun-lai; YANG Fan; XIA Ji-xing; WANG Ke-qin; LIU Xing-long
Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086
关键词:
Keywords:
分类号:
S476
DOI:
1002-2767（2015）09-0064-05
文献标志码:
A
摘要:
为获得具有高效、广谱拮抗作用的生防细菌。从实验室及葡萄、黄瓜、西红柿的根和根际土壤中培养得到282株细菌，以13种常见植物病原真菌为靶标菌，通过平板对峙法和琼脂扩散法测定了分离细菌的发酵液和发酵滤液对靶标菌的抑制效果。结果表明：共有9株分离细菌具有较好的抑制作用，其中4株拮抗细菌的发酵液对所有13株靶标病原菌均有很好的抑制作用，表现出较为广谱的抑菌作用。其中k8j菌株的抑菌效果与其它拮抗菌株达到了显著性水平，其对玉米大斑病菌（Setosphaeria turcica）的抑制率最高，达到了92.02%。9株拮抗细菌的发酵滤液对不同的靶标病原菌也表现出一定的抑制作用，其中HMGR-2菌株对玉米大斑病菌的抑制效果最好，抑菌圈直径达到了4.15 cm；菌株k8j的发酵滤液对11株病原菌均具有很好的抑菌效果（除水稻稻瘟病菌和番茄灰霉病菌外），并与其它拮抗菌株比较达到了显著性水平，说明该菌株的代谢产物也具有较为广谱的抑菌效果。
Abstract:
In order to obtain bio-control bacteria with highly effective and broad spectrum antagonism effect against plant pathogenic fungi,282 isolates were obtained by the method of dilution coated tablet and streak plate from lab and roots and rhizosphere soil samples of grapes,tomatoes and cucumbers.With 13 kinds of common plant pathogenic fungi as indicators,inhibitory effect of fermentation liquid and fermentation filtrate against the indicators were tested by the methods of tablet confrontation and agar disk diffusion.The results showed that 9 bacterial strains had efficient and stable inhibitory activity,and 4 strains of them fermentation liquid had efficient inhibitory activity against all the indicators,showing broad antimicrobial spectrum against pathogenic fungi.Among them,the inhibition effect of k8j was significantly higher than other isolates,and it had the highest inhibition effect against Setosphaeria turcica,with the inhibition rate was 92.02%.9 bacterial strains fermentation filtrate also showed some inhibitory effect.HMGR-2 had the best inhibition effect against Setosphaeria turcica?with the inhibition zone diameters was 4.15 cm.Fermentation filtrate of k8j had good inhibition effect against 11 indicators (except for Pyricularia grisea?and Botrytis cinerea),and compared with other strains,its inhibitory action reached the significant level,and it showed that metabolites also had broad spectrum of antibacterial effect.

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备注/Memo

收稿日期：2015-04-23

基金项目：国家高技术研究发展计划（863计划）资助项目（2011-AA10A205）；黑龙江省农业科技创新工程资助项目（2014-ZD012）
第一作者简介：王爽（1981-），女，黑龙江省哈尔滨市人，硕士，助理研究员，从事微生物农药和生物防治研究。E-mail：wslovegyn0@163.com。
通讯作者：李新民(1964-)，男，甘肃省成县人，硕士，研究员，从事微生物农药和生物防治研究。

更新日期/Last Update: 2016-07-13