LIU Hua-ming,DONG Zhi-bo,SUI Xiao-kai,et al.Optimization of RNA Extraction Technology for Cold Region Rice[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(07):9-11.[doi:10.11942/j.issn1002-2767.2015.07.0009]
寒地水稻优质RNA提取技术优化
- Title:
- Optimization of RNA Extraction Technology for Cold Region Rice
- 文章编号:
- 1002-2767（2015）07-0009-03
- Keywords:
- rice(Oryza sativa); RNA; trizol; RT-PCR
- 分类号:
- S511
- 文献标志码:
- A
- 摘要:
-
空育131和稻花香是黑龙江省很有代表性的寒地栽培品种，也是育种研究的重要材料。为了获取此品种的优质RNA，以经低温避光处理3 d的空育131及稻花香水稻的10日龄水稻苗叶为材料，利用优化的UNIQ-10柱式Total RNA Extractor（Trizol）试剂盒法提取RNA。之后，进行RT-PCR反应，并对RT-PCR的结果进行琼脂糖凝胶电泳的检测。结果表明：通过优化，改进了UNIQ-10柱式Total RNA Extractor（Trizol）试剂盒法，提取的RNA纯度较高，A260?/ A280均接近1.8，并且RT-PCR的条带清晰。
- Abstract:
- ?Kongyu131 and Daohuaxiang are typical cultivars in Heilongjiang province.And they are important materials of breeding research.In order to obtain their high-quality RNA,Kongyu131 and Daohuaxiang rice seedling leaves were used as materials,and treated with low temperature away from light for three days,whose age were ten days.Optimization of UNIQ-10 pillar TotalRNA Extractor (Trizol) kits was used to extract RNA.After RT-PCR reaction,and the results of RT-PCR by detection of agarose gel electrophoresis.The result showed that by the optimization,the UNIQ-10 pillar TotalRNA Extractor (Trizol) kits could obtain RNA with high purity,whose A260 / A280?was close to 1.8,and stripe of RT-PCR was clear.
参考文献/References:
[1]Hannon G J.RNA interference[J].Nature,2002,418(6894):244-251.
相似文献/References:
[1]迟莉.11%多·咪·福美双悬浮种衣剂防治水稻恶苗病田间药效试验[J].黑龙江农业科学,2013,(02):59.
CHI LI.The Field Efficacy Trial of 11% Carbendazim?Prochloraz?Thiram FSC to Prevent Rice Bakanae Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):59.
[2]王玉洋,蒋柳青,董雪梅,等. 不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(01):11.
WANGYu-yang,JIANGLiu-qing,DONGXue-mei,et al.EffectofDifferentFactorsonRiceCallusFormation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):11.
[3]丁佳红,薛正莲,杨超英. 水杨酸对铜胁迫下水稻幼苗膜脂过氧化作用的影响[J].黑龙江农业科学,2013,(01):14.
DINGJia-hong,XUEZheng-lian,YANGChao-ying.EffectofSalicylicAcidonMembraneLipidPeroxidationinRiceSeedlingsunderCopperStress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):14.
[4]王岩成,胡焕春,岳萍. 一定体积的水稻种子间隙吸水量的定量试验[J].黑龙江农业科学,2013,(01):27.
WANGYan-cheng,HUHuan-chun,YUEPing.QuantitativeTestofRiceSeedsGapWaterAbsorptionofCertainVolume[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(07):27.
[5]解忠.不同温度对水稻灌浆期籽粒淀粉关键酶活性及稻米品质的影响[J].黑龙江农业科学,2014,(07):32.
XIE Zhong.Effect of Different Temperature on Grain Starch Key Enzyme Activity and Rice Quality at Filling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):32.
[6]张智杰.牡丹江地区水稻生产现状及发展趋势[J].黑龙江农业科学,2014,(07):144.
ZHANG Zhi-jie.Present Situation and Development Trends of Rice Production in Mudanjiang Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):144.
[7]张智杰.水稻粒形性状的QTL分析[J].黑龙江农业科学,2014,(06):15.
LIN Hong,SUN Dequan,LI Suiyan,et al.Evaluation on Induction Rate of Maize Haploid Derived ?Lines for Different Groups F1[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):15.
[8]曾宪国,项洪涛,王立志,等.孕穗期不同低温对水稻空壳率的影响[J].黑龙江农业科学,2014,(06):19.
ZENG Xianguo,XIANG Hongtao,WANG Lizhi,et al.Effect of Different Low Temperature on the Percentage of Rice Unfilled Grains in Booting Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):19.
[9]白 雪,郑桂萍,王宏宇,等.寒地水稻侧深施肥效果的研究[J].黑龙江农业科学,2014,(06):40.
BAI Xue,ZHENG Guiping,WANG Hongyu,et al.Research on the Effect of Side and Deep Fertilizing for Rice in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):40.
[10]王晓东,王茂青,王红霞.寒地水稻种质资源产量构成因素与产量的关系研究[J].黑龙江农业科学,2014,(06):5.
WANG Xiao-dong,WANG Mao-qing,WANG Hong-xia.Analysis on the Relationship Between Yield Components and Yield of Rice Germplasm in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(07):5.
备注/Memo
收稿日期：2015-03-02