GAO Feng-mei,SHAO Li-gang,WANG Yan,et al.Genotype Identification and Screening of Hybrid Early Generation of Waxy Spring Wheat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2015,(06):1-4.[doi:DOI:10.11942/j.issn1002-2767.2015.06.0001]
糯性春小麦杂交早代的基因型鉴定与筛选
- Title:
- Genotype Identification and Screening of Hybrid Early Generation of Waxy Spring Wheat
- 文章编号:
- 1002-2767（2015）06-0001-04
- Keywords:
- waxy spring wheat; hybrid early generation; waxy protein
- 分类号:
- S512
- 文献标志码:
- A
- 摘要:
- 为筛选出糯性小麦材料，研究糯性小麦籽粒的出现频率,明确各分离群体的Wx基因型，使筛选的糯性与非糯性材料呈现出表现型与基因型的统一，以生产上大面积推广应用的普通小麦品种克旱16、克春5号为母本，糯性小麦品种宁糯1号、南农09P18为父本4个杂交组合的F2代分离群体为研究对象，以半籽粒碘染色与分子检测法为研究手段，对F2分离群体进行糯与非糯性材料鉴定与筛选。结果表明：组合克旱16/宁糯1号、克春5号/宁糯1号、克旱16/南农09P18、克春5号/南农09P18糯性小麦的出现频率分别为1.1%、1.4%、1.4%、1.3%；从４个组合的F2 群体100个单株中筛选到了共11株Waxy基因全缺型的植株，与碘染色结果一致。
- Abstract:
- In order to screen waxy wheat ,study frequency of waxy wheat grain,determine Wx genotype of segregation population ,presented unification of phenotype and genotype ,taking wheat varieties( Kehan 16 and Kechun 5) as female parents,waxy wheat varieties(Ningno 1,Nannong 09P18) as male parents,identification and screen of waxy and nonwaxy wheat from F2 separation population of 4 cross combinations was studied with half grain idodine dyeing and molecular examination .The results indicated that the waxy wheat appearance frequency of Kehan 16/Ningnuo 1,Kechun 5/Ningnuo 1,Kehan 16/Nannong 09P18 and Kechun 5/Nannong 09P18 were 1.1%,1.4%,1.4%,and 1.3% respectively.there was 11 plants with waxy genes all deletion were screened from 100 individual plants of F2 generation population of four cross combinations by using the Wx molecular mark identification,this result obtained the confirmation by the idodine dyed way. waxy spring wheat; hybrid early generation; waxy protein （该文作者还有刘宁涛 、邹东月、王志坤、 程睿钰，单位同第一作者）
参考文献/References:
［1］陈新民.糯小麦（Waxy Wheat）研究进展[J]．麦类作物学报，2000,20(3):82-85.
[2]Miura H,Sugawara A.Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm[J].Theoretical and Applied Genetics,1996,93(7):1066-1070.
[3]Nakamura T,Yamamori M,Hirano H,et al.Production of waxy(amylose free) wheats[J].Molecular and General Genetics,1995,248(3):253-259.
[4]Yasui T,Sasaki T,Matsuki J.Milling and flour pasting properties of waxy endosperm mutant lines of bread wheat(Triticum aestivum L)［J］.J.Sci.Food Agric.1999,79: 687-692.
[5]Zeng M,Morris C F,Batey I L,et al.Sources of variation for starch gelatinization ,pasting，and gelation properties in wheat[J].Cereal Chemistry，1997,74(1)：63-71.
[6]Hayakawa K,Tanaka K,N akamura T,et al.Quality characteristics of waxy hexap loid wheat:P roperties of starch gelatinizationand retrogradation[J].Cereal Chemistry,1997,74(5):576-580.
[7]Zhao X C,Sharp P J.An improved 1DSDSPAGE method for the identification of three waxy bread wheat waxy protein.J[J].Cereal Sci,1996,23:191-193.
[8]Nakamura T,Yamamori M,Hirano H，et al.Decrease of waxy protein in two wheat cultivars with low amylose contents[J].Plant Breeding,1993,111:99-105.
[9]Miura H,Tani S.Endosperm starch properties in several wheat varities preferred for Jappanese noodles[J].Euphytica,1994,72:171-175.
[10]Mirua H,Sugawara A.Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm[J].Theoretical Appl Genetics,1996,93(7):1066-1070.
[11]陈新民.糯小麦（Waxy Wheat）研究进展[J]．麦类作物学报，2000,20(3):82-85.
[12]李中安,宁锟.糯小麦研究初报[J].麦类作物学报,2001,21(3):95-96.
[13]梁荣奇,张义荣,刘守斌，等.利用Wx基因分子标记辅助选择培育糯小麦[J].遗传学报,2001,28 (9):856-863.
备注/Memo
收稿日期：2015-01-12
基金项目：国家现代农业小麦产业技术体系东北综合试验站资助项目（CARS-03）；黑龙江省农业科学院创新工程资助项目
第一作者简介：高凤梅（１９７１-），女，山东省寿光市人，在读博士，副研究员，从事小麦遗传育种研究。Ｅ-ｍａｉｌ：Fengmeigao2007@163.com。