[1]白郁山,刘 政,张 丹,等.异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物的影响[J].黑龙江农业科学,2014,(01):118-120.
　BAI Yu-shan,LIU Zheng,ZHANG Dan,et al.Effect of Isoleucine on Extracellular Polymeric Substance of Acidithiobacillus ferrooxidans[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):118-120.
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异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物的影响

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2014年01 页码: 118-120 栏目: 出版日期: 2014-01-10
Title:
Effect of Isoleucine on Extracellular Polymeric Substance of Acidithiobacillus ferrooxidans
文章编号:
1002-2767（2014）01-0118-03
作者:
白郁山; 刘 政; 张 丹; 龚权君; 刘 丹; 刘晓梅
辽宁工程技术大学 理学院，辽宁 阜新 123000
Author(s):
BAI Yu-shan; LIU Zheng; ZHANG Dan; GONG Quan-jun; LIU Dan; LIU Xiao-mei
College of Science，Liaoning Technical University，Fuxin,Liaoning 123000
关键词:
嗜酸氧化亚铁硫杆菌; 生物预氧化; 胞外多聚物; 异亮氨酸
Keywords:
Acidithiobacillus ferrooxidans; biological pre-oxidation; extracellular polymeric substances; isoleucine
分类号:
TF18
文献标志码:
A
摘要:
为提高生物冶金效率，研究了添加异亮氨酸对嗜酸氧化亚铁硫杆菌胞外多聚物组分、嗜酸氧化亚铁硫杆菌生长和吸附能力的影响。结果表明：与对照相比，添加异亮氨酸的嗜酸氧化亚铁硫杆菌生长周期明显缩短，Fe2+的耗尽时间缩短，培养后期Fe2+剩余量明显降低。最高菌密度提高17%，环境pH降低20.45%，胞外多聚物中蛋白质组分提高16.84%，多糖组分提高5.05%。培养时间为72 h时，添加异亮氨酸的嗜酸氧化亚铁硫杆菌菌液EPS吸附量为54.520 8 μg·mL-1，比对照提高了44.05%。整个生长周期中，异亮氨酸对菌液中游离EPS含量影响不大。
Abstract:
In order to improve the efficiency of biological metallurgy，the effect of isoleucine added to the liquid medium on extracellular polymeric component of Acidithiobacillus ferrooxidans，growth and adsorption capacity were studied.The results showed that compared with CK，growth cycle of Acidithiobacillus ferrooxidans with isoleucine and consumption time of Fe2+ were shortened，the highest cell density increased by 17%，pH of medium decreased by 20.45%,protein content of extracellular polymeric increased by 16.84%,polysaccharide content increased by 5.05%.The adsorption amount of EPS added isoleucine was 54.520 8 μg·mL-1 for 72 h,increased by 44.05% than CK.During the whole growth cycle,isoleucine had little effect on dissociation of EPS.

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备注/Memo

第一作者简介：白郁山（1975-），男，辽宁省辽阳市人，硕士，讲师，从事微生物冶金研究。E-mail：903551042@qq.com。

更新日期/Last Update: 2014-12-07