MA Wen-dong.Study on Extraction Method of Rice Genomic DNA[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):7-10.
水稻基因组DNA提取方法的研究
- Title:
- Study on Extraction Method of Rice Genomic DNA
- 文章编号:
- 1002-2767（2014）01-0007-04
- Keywords:
- rice; genomic DNA; CTAB; SDS; molecular marker-assisted breeding
- 分类号:
- S511.035.3
- 文献标志码:
- A
- 摘要:
- 为探索分子标记辅助选择育种中快速有效的DNA提取方法，以水稻新鲜叶片、衰老叶片、水稻种子为材料，以CTAB法和SDS法为提取方法，利用紫外分光光度计和琼脂糖凝胶电泳进行DNA纯度检测，对水稻基因组DNA提取方法和实验体系进行了优化和摸索。结果表明：新鲜叶片提取效果最好，其次是种子，再次是衰老叶片；CTAB法和SDS法差别不大；为避免酚类物质氧化污染，可在提取液中添加β-巯基乙醇。
- Abstract:
- In order to study fast and effective extraction method of rice genomic DNA for molecular marker-assisted breeding,taking rice fresh leaves,senescing leaves and rice seeds as experimental materials and CTAB and SDS as methods,DNA purity was tested by UV spectrophotometer and agarose gel electrophoresis,extraction methods and experimental system of rice genomic DNA were optimized and explored.The results showed that quality extracted from fresh leaves was the best followed by seeds and senescing leaves was the last;the difference between CTAB and SDS methods was not very obvious,and β-mercaptoethanol could added into solution to avoide the pollution of phenols oxidation.
参考文献/References:
[1]李雪林,梁华,张泽民,等.分子标记技术在植物遗传育种中的进展[J].河南科技大学学报,2004,24(2):69-72. [2]周锦霞,敖光辉,魏琴,等.分子标记辅助选择及其在植物抗病育种中的作用[J].宜宾学院学报,2005(6):90-92. [3]黄文坤,郭建英,万方浩.AFLP标记在植物遗传多样性研究中的应用[J].农业生物技术科学,2006,22(8):50-54. [4]黄小群.一份新的水稻黄化突变体的遗传分析及基因定位[D].雅安：四川农业大学,2006:27-28. [5]黄龙花,吴清平,杨小兵，等.基于特定引物PCR的DNA分子标记技术研究进展[J].生物技术通报,2011(2):62-63. [6]张立荣,徐大庆,刘大群.SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):89-94. [7]刘之熙,陈祖武,朱克永,等.利用SSR分子标记快速鉴定杂交水稻种子纯度技术体系的优化[J].杂交水稻,2008,23(1):60-63. [8]雷东阳,陈立云,姜琴.分子标记辅助选择及其在水稻育种中的应用[J].作物研究,2005(5):257-260. [9]孙川.水稻基因组DNA简易制备方法研究以及分子标记辅助选择改良稻米品质[D].扬州：扬州大学,2010：21-23. [10]易庆平,罗正荣,张青林.植物总基因组DNA提取纯化方法综述[J].安徽农业科学,2007,35(25):7789-7791. [11]张宁,王凤山.DNA提取方法进展[J].中国海洋药物,2004(2):40-46. [12]朱世杨,罗天宽,张小玲,等.8种水稻基因组DNA提取方法的比较[J].安徽农业科学,2009,37(5):1929-1931. [13]王景雪,孙毅,高武军.一种简便实用的植物总DNA提取方法[J].山西大学学报,2000,23(3):271. [14]桑贤春,何光华,张毅，等.水稻PCR扩增模板的快速制备[J].遗传,2003,25(6):705-707. [15]楼巧君,陈亮,罗利君.三种水稻基因组DNA快速提取方法的比较[J].分子植物育种,2005,3(5):749-752. [16]John M E.An efficient method for isolation of RNA and DNA from plants containing polyphenolics[J].Nucleic acids research,1992,20(9):2381. [17]闫双勇,苏京平,王胜军.一种同时适用于水稻种子和叶片的简单快速DNA提取方法[J].中国稻米,2011,17(5):11-13. [18]袁云香,李海娟.水稻基因组DNA提取方法的研究进展[J].湖北农业科学,2010,49（4）:968-971.
相似文献/References:
[1]迟莉.11%多·咪·福美双悬浮种衣剂防治水稻恶苗病田间药效试验[J].黑龙江农业科学,2013,(02):59.
CHI LI.The Field Efficacy Trial of 11% Carbendazim?Prochloraz?Thiram FSC to Prevent Rice Bakanae Disease[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):59.
[2]王玉洋,蒋柳青,董雪梅,等. 不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(01):11.
WANGYu-yang,JIANGLiu-qing,DONGXue-mei,et al.EffectofDifferentFactorsonRiceCallusFormation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):11.
[3]丁佳红,薛正莲,杨超英. 水杨酸对铜胁迫下水稻幼苗膜脂过氧化作用的影响[J].黑龙江农业科学,2013,(01):14.
DINGJia-hong,XUEZheng-lian,YANGChao-ying.EffectofSalicylicAcidonMembraneLipidPeroxidationinRiceSeedlingsunderCopperStress[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):14.
[4]王岩成,胡焕春,岳萍. 一定体积的水稻种子间隙吸水量的定量试验[J].黑龙江农业科学,2013,(01):27.
WANGYan-cheng,HUHuan-chun,YUEPing.QuantitativeTestofRiceSeedsGapWaterAbsorptionofCertainVolume[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(01):27.
[5]解忠.不同温度对水稻灌浆期籽粒淀粉关键酶活性及稻米品质的影响[J].黑龙江农业科学,2014,(07):32.
XIE Zhong.Effect of Different Temperature on Grain Starch Key Enzyme Activity and Rice Quality at Filling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):32.
[6]张智杰.牡丹江地区水稻生产现状及发展趋势[J].黑龙江农业科学,2014,(07):144.
ZHANG Zhi-jie.Present Situation and Development Trends of Rice Production in Mudanjiang Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):144.
[7]张智杰.水稻粒形性状的QTL分析[J].黑龙江农业科学,2014,(06):15.
LIN Hong,SUN Dequan,LI Suiyan,et al.Evaluation on Induction Rate of Maize Haploid Derived ?Lines for Different Groups F1[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):15.
[8]曾宪国,项洪涛,王立志,等.孕穗期不同低温对水稻空壳率的影响[J].黑龙江农业科学,2014,(06):19.
ZENG Xianguo,XIANG Hongtao,WANG Lizhi,et al.Effect of Different Low Temperature on the Percentage of Rice Unfilled Grains in Booting Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):19.
[9]白 雪,郑桂萍,王宏宇,等.寒地水稻侧深施肥效果的研究[J].黑龙江农业科学,2014,(06):40.
BAI Xue,ZHENG Guiping,WANG Hongyu,et al.Research on the Effect of Side and Deep Fertilizing for Rice in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):40.
[10]王晓东,王茂青,王红霞.寒地水稻种质资源产量构成因素与产量的关系研究[J].黑龙江农业科学,2014,(06):5.
WANG Xiao-dong,WANG Mao-qing,WANG Hong-xia.Analysis on the Relationship Between Yield Components and Yield of Rice Germplasm in Cold Region[J].HEILONGJIANG AGRICULTURAL SCIENCES,2014,(01):5.
备注/Memo
基金项目：国家“十二五”科技支撑计划资助项目（2011BAD35B02-01-01，2012BAD04B01-01-01）