[1]任洪雷,陶波,李春霞.氯嘧磺隆降解菌F31发酵条件及降解能力的研究[J].黑龙江农业科学,2013,(09):45-48.
　REN Hong-lei,TAO Bo,LI Chun-xia.Study on Fermentation Conditions and Degradation Ability of Degrading Fungi F31 of Chlorimuron-ethyl[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(09):45-48.
点击复制

氯嘧磺隆降解菌F31发酵条件及降解能力的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2013年09 页码: 45-48 栏目: 出版日期: 2013-09-30
Title:
Study on Fermentation Conditions and Degradation Ability of Degrading Fungi F31 of Chlorimuron-ethyl
文章编号:
1002-2767（2013）09-0045-04
作者:
任洪雷1; 2; 陶波2; 李春霞1
1.黑龙江省农业科学院 玉米研究所，黑龙江 哈尔滨 150086；
2.东北农业大学 农学院，黑龙江 哈尔滨 150030
Author(s):
REN Hong-lei1; 2; TAO Bo2; LI Chun-xia1
1.Maize Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;
2.College of Agriculture,Northeast Agricultural University,Harbin，Heilongjiang 150030
关键词:
氯嘧磺隆; 降解真菌F31; 摇瓶培养; 降解能力
Keywords:
chlorimuron-ethyl; degrading fungi F31; shake flask; degradation ability
分类号:
S182
文献标志码:
A
摘要:
为促进和加快降解菌的工业化生产，解决氯嘧磺隆残留药害问题，利用单因素筛选结合正交试验的方法，以氯嘧磺隆降解菌F31的菌体生长量（OD600）为测定指标，系统研究了不同碳源、氮源及无机盐的发酵结果。结果表明：单因素试验初步筛选出碳源葡萄糖，氮源蛋白胨和硫酸铵，无机盐KH2PO4、NaCl、MgSO4、FeSO4利于降解菌生长。在此基础上通过L18（37）的正交试验，确定了最优培养基配方为葡萄糖2%、蛋白胨1%、硫酸铵1%、KH2PO40.07%、NaCl 0.2%、MgSO40.07%、FeSO40.000 5%；最优配方培养基摇瓶降解能力试验表明：降解菌F31在新培养基中生长状态良好，降解能力优良，培养36 h液相色谱条件下未检出氯嘧磺隆。
Abstract:
In order to promote and accelerate industrialization of degrading fungi,solve the residual phytotoxicity of chlorimuron-ethyl,with the single factor screening and orthogonal test,fermentation of different carbon sources,nitrogen sources and inorganic chemicals were systematically studied with index of degrading fungi growth(OD600).The results showed that in the single factor test,carbon source glucose,nitrogen source peptone and (NH4)2SO4,inorganic salts KH2PO4,NaCl,MgSO4 and FeSO4 were primarily screened out,which were benefit for the growth of the degrading fungi.Based on the single factor test,optimal carbon sources,nitrogen sources and inorganic chemicals were chosen to be component factors,and each of them was operated with orthogonal optimization test by three levels,thereby achieving optimum culture medium formula which was 1% glucose,2% peptone,1%（NH4）2SO4,0.07% KH2PO4,0.2% NaCl，0.07% MgSO4 and 0.000 5% FeSO4.The best medium shake flask tests showed that F31 could grow well in the new medium and the degradation ability was strong,chlorimuron-ethyl with HPLC wasn’t detected after 36 h.

参考文献/References:

[1]陶波,苏少泉.农作物磺酰脲类除草剂耐性的研究[J].东北农业大学学报,1995（5）:10-20.
[2]邓金保.磺酰脲类除草剂综述[J].世界农药,2003,25(3):24-32.
[3]陶波，何钟佩.豆磺隆在土壤中残留动态及对后茬作物的影响[J].西北植物学报，2000，20（6）：1229-1233.
[4]Madan Joshi，Hugh M Brown.Degradation of chlorsulfuronby soil microorganism[J].Weed Science,1985,33:888-893.
[5]Vega D，Bastide J,Poulain C.Chemical or microbial degradation of sulfonylureas in the soilⅡ:Metsulfuron-methy[J].Weed Res.,1992,32:149-155.
[6]滕春红，苏少泉.除草剂在土壤中的微生物降解及污染土壤的生物修复[J].农药，2006，45（8）：505-507. [7]刘辉，陶波.农药生物降解剂对氯嘧磺隆残留解毒效应的研究[J].微生物学报，2003（3）：36-38.
[8]滕春红，李晓薇，陶波.氯嘧磺隆降解真菌的分离和鉴定[J].东北农业大学学报，2008，39(12):19-22.
[9]钱存柔，黄仪秀.微生物学实验教程[M].北京：北京大学出版社，1999：12-22.
[10]陈天寿.微生物培养基的制造与应用[M].北京：中国农业出版社，1995：30-43.
[11]李治祥.苯磺隆?甲磺隆?氯磺隆混合物的液相色谱分析[J].农药科学与管理，2002,23(1):17-19.
[12]Claudia Sheedy，Hall J Christopher.Immunoaffinity purufication of chlorimuron ethyl from soil extracts prior to quantitation by enzyme-linked immunosorbent assay[J].Agric.Food Chem.,2001，49:1151-1157.

相似文献/References:

[1]王彦杰,洪秀杰,宋伟英,等.氯嘧磺隆降解菌降解效果研究[J].黑龙江农业科学,2012,(06):73.
　WANG Yan-jie,HONG Xiu-jie,SONG Wei-ying,et al.Research on Degradation Effect of Chlorimuronethyl Degradable Bacteria[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(09):73.

备注/Memo

收稿日期：2013-04-18
基金项目：哈尔滨市科技攻关资助项目（2007AA6CN097）
第一作者简介：任洪雷（1982-），男，黑龙江省肇源县人，硕士，研究实习员，从事玉米遗传育种研究。E-mail:hlren2008@yahoo.cn。

更新日期/Last Update: 2014-10-31