QIN Guo-le,QIN Wen-yuan,WEN Fang,et al.Aseptic seeding，Tissue Culture and Rapid Propagation of Primulina hochiensis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(08):14-17.
河池报春苣苔的无菌播种、组织培养和快速繁殖
- Title:
- Aseptic seeding，Tissue Culture and Rapid Propagation of Primulina hochiensis
- 文章编号:
- 1002-2767（2013）08-0014-04
- 分类号:
- S682.303.8
- 文献标志码:
- A
- 摘要:
- 为研究河池报春苣苔的组培快繁技术，以河池报春苣苔的果实为外植体，通过比较不同的植物生长调节剂种类、浓度配比、生根培养基类型以及生根苗的移栽基质,建立河池报春苣苔织培养快速繁殖体系。结果表明,外植体表面消毒以75%酒精预处理1 min,再用0.1% HgCl2浸泡15 min。培养基1/2MS+NAA 0.1 mg.L-1+6-BA 0.15 mg.L-1利于种子萌发，形成不定芽，形成丛生芽,增殖与继代培养基MS+6-BA 0.5 mg.L-1+IBA 0.1 mg.L-1或MS用于继代增殖,30 d的增殖倍数为3~4倍;1/2MS+IBA 0.1 mg.L-1适宜诱导生根获得再生植株,生根率100%;生根苗适宜移栽于混合基质（碎木屑∶园土∶沙=7∶2∶1)中,一个月后成活率为90%。
- Abstract:
- The techniques of tissue culture and rapid propagation of Primulina hochiensis were studied by explants,comparing with different types and concentrations of plant growth regulators,types of rooting medium and transplanting medium.The results showed that the better suitable procedure for sterilization was to soak explants in 0.1% HgCl2solution for 15 min after pretreatment with 75% alcohol for 1 min,the seeds were the best explants.1/2MS+NAA0.1 mg?L-1+6-BA0.15 mg.L-1could effectively induce fasciculate buds,MS+6 -BA0.5 mg.L-1+IBA0.1 mg.L-1or MS was suitable for subculture and proliferation multiples was 3~4 times every 30 d;the rooting medium was 1/2MS+IBA 0.1 mg.L-1,and the rooting rate was 100%.The rooting seedlings were effectively transplanted in mixed matrix soil（sawdust∶garden soil∶sand=7∶2∶1）,the highest survival rate was 90% after a month.
参考文献/References:
[1]Weber A,Middleton D J,Forrest A,et al.Molecular systematics and remodelling of Chirita and associated genera(Gesneriaceae)[J].Taxon 2011，60(3):767-790.
[2]WEN Fang,QIN Guo-le,WEI Yi-gang,et al.Primulina hochiensis var.rosulata(Gesneriaceae)―a new variety at an entrance of a limestone cave from Guangxi,China[J].Phytotaxa,2012，54:37-42.
[3]温放，李湛东.广西苦苣苔科植物资源及产业化前景[J].广西园艺，2005,16（4）：57-59.
[4]李振宇，王印政.中国苦苣苔科植物[M].郑州:河南科学技术出版社，2004:13-677.
[5]韦毅刚，温放，Michael M ller，等.华南苦苣苔科植物[M].南宁广西科学技术出版社，2010：255-257.
[6]汤正辉，陈维伦，石雷，等.刺齿唇柱苣苔的离体快速繁殖[J].植物生理学通讯,2004,40(2):211.
[7]温放，李湛东，张启翔.牛耳朵的离体培养和快速繁殖[J].植物生理学通讯,2006,42(5):906.
[8]温放，张启翔，任翔翔.黄花牛耳朵的离体培养和快速繁殖[J].植物生理学通讯,2008,44(2):301-302.
[9]李翠，凌征柱，姚绍嫦，等.苦苣苔科植物组织培养研究进展[J].安徽农业科学,2010,38(31):17387-17388,17390.
[10]梁桂友，温放，李湛东.尖萼唇柱苣苔的组织培养和快速繁殖[J].植物生理学通讯,2007,43(2):321.
[11]余海霞，凌征柱，黄雪彦，等.三苞唇柱苣苔的组织培养与快速繁殖[J].植物生理学通讯,2010,46(11):1177-1178.
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备注/Memo
收稿日期：2013-04-24
基金项目：基金项目广西自然科学基金资助项目（2012 GXNSFBA053056）；河池学院科研课题资助项目(2008QS-N003)
第一作者简介：覃国乐（1969-），男，广西壮族自治区罗城市人，硕士，讲师，从事野生动植物保护与利用研究。E-mail:qinguole2005@sina.com。
通讯作者：温放（1976-），男，广东省梅县人，博士，助理研究员，从事植物分类、植物资源与植物地理学研究。E-mail:wenfang760608@139.com。