GUO Hui-juan,LI Gai-li.Effects of Propagation Condition on Growth and Development of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Caragana korshinskii[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(12):44-47.
扩繁条件对柠条土著AM真菌生长发育的影响
- Title:
- Effects of Propagation Condition on Growth and Development of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Caragana korshinskii
- 文章编号:
- 1002-2767（2012）12-0044-04
- Keywords:
- host plant; culture substrate; arbuscular mycorrhizal fungi; spore density; mycorrhizal colonization
- 分类号:
- Q948.11
- 文献标志码:
- A
- 摘要:
- 利用盆栽试验，设置玉米（Zea mays L.）和三叶草（Trifolium repens L.）2种宿主植物和3种培养基质（沙土和农田土质量比分别为1∶1,2∶1和3∶1），研究了宿主植物和培养基质对柠条（Caragana korshinskii Kom.）根围土著AM真菌生长发育的影响。结果表明：宿主植物为玉米，培养基质中沙土与农田土质量比为3∶1时，AM真菌菌根侵染率、孢子密度、根内菌丝琥珀酸脱氢酶活性和菌根长度均为最高。说明以玉米为宿主植物，沙土与农田土质量比为3∶1的盆栽基质对柠条根围土著AM真菌的生长发育最为有利。
- Abstract:
- The effects of different host plants(Zea mays L.and Trifolium repens L.)and culture substrates(the mass ratio of sand to soil was 1∶1,2∶1 and 3∶1,respectively)on growth and development of arbuscular mycorrhizal(AM)fungi in the rhizosphere of Caragana korshinskii were studied using pot experiment.The results showed that mycorrhizal colonization,spore density,succinate dehydrogenase activity of intraradical hypha and mycorrhizal length were the highest when the host plant was Zea mays under the substrate(mass ratio of sand to soil was 3∶1).Therefore,Zea mays as host plant and culture substrate(mass ratio of sand to soil was 3∶1)were the best for the growth and development of indigenous AM fungi in the rhizosphere of C. korshinskii.
参考文献/References:
[1]Koide R T,Mosse B A.History of research on arbuscular mycorrhiza[J].Mycorrhiza,2004,14:145 - 163. [2]贺学礼，高露，赵丽莉.水分胁迫下丛枝菌根AM真菌对民勤绢蒿生长和抗旱性的影响[J].生态学报，2011，31（4）：1029-1037. [3]Wu Qiangsheng,He Xinhua,Zou Yingning,et al.Spatial distribution of glomalin-related soil protein and its relationships with root mycorrhization,soil aggregates,carbohydrates,activity of protease and β-glucosidase in the rhizosphere of Citrus unshiu[J].Soil Biology and Biochemistry,2912,45:181-183. [4]Gianinazzi S,Vosátka M.Inoculum of arbuscular mycorrhizal fungi for production systems:science meets business[J].Canadian Journal of Botany,2004,82:1264-1271. [5]温莉莉，梁淑娟，宋鸽.丛枝菌根(AM)真菌扩繁方法的研究进展[J].东北林业大学学报，2009，37（6）：92-96. [6]龙良鲲，姚青，羊宋贞，等.扩繁条件对2种AMF菌剂接种势的影响[J].微生物通报，2007，34（2）：204-207. [7]陈宁，王幼珊，蒋家珍，等.培养基质对丛枝菌根(AM)真菌生长发育的影响[J].农业工程学报，2007，23（2）：205-207. [8]Phillips J M,Hayman D S.Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J].Transactions of the British Mycological Society,1970,55:158-161. [9]Smith S E,Gianinazzi-Pearson V.Phosphate uptake and vesicular arbuscular activety in mycorrhizal Allium cepa L.Effect of Photon irradiance and phosphate nutrition[J].Australian Journal of Plant Physiology,1990,17:7-188. [10]王学奎.植物生理生化实验原理和技术[M].北京：高等教育出版社，2006：105-281. [11]中国土壤学会农业化学专业委员会.土壤农业化学常规分析方法[M].北京：科学出版社，1983：67-101. [12]Ianson D C,Allen M F.The effects of soil texture on extraction of vesicular arbuscular mycorrhizal spores from arid soil[J].Mycologia,1986,78:164-168. [13]Tahat M M,Kamaruzaman S,Radziah O,et a1.Plant host selectivity for multiplication of Glomus mosseae spore[J].International Journal of Botany,2008,4:66-470. [14]孙向伟，王晓娟，陈牧，等.生态环境因子对AM真菌孢子形成与分布的作用机制[J].草业学报，2011，20（1）：214-221. [15]石兆勇，刘润进，李瑞卿.栽培基质与AM真菌对园艺作物的影响[J].中国生态农业学报，2002，10（2）：50-52. [16]陈宁，王幼珊，李晓林，等.宿主植物栽培密度对AM真菌生长发育的影响[J].菌物系统，2003，22（1）：88-94. [17]姚青，胡又厘，廖金才，等.宿主植物的栽培密度对AM真菌孢子产量的影响[J].华南农业大学学报，2007，28（1）：14-17. [18]Leake J R,Johnson D,Donnelly D,et al.Networks of power and influence:the role of mycorrhizal mycelium in controlling plant communities and agroecosystem functio-ning[J].Canadian Journal of Botany,2004,82:1016-1045.
备注/Memo
基金项目：保定市科学技术研究与发展指导计划资助项目（11ZN032）