LAN Wei,WAN Ling-yun,MA Zong-xin,et al.Study on Tissue Culture of Three Varieties of Strawberry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(08):21-24.
三个草莓品种的组织培养研究
- Title:
- Study on Tissue Culture of Three Varieties of Strawberry
- 文章编号:
- 1002-2767（2012）08-0021-04
- Keywords:
- strawberry; stem section; proliferation; rooting
- 分类号:
- S668.4
- 文献标志码:
- A
- 摘要:
- 为生产出高产优质草莓，提高效益，以草莓达斯、弗州、丰香3个品种为试材研究草莓茎段离体再生。以MS为基本培养基，采用添加不同浓度梯度的植物生长调节剂对草莓幼苗进行了丛芽诱导、试管苗增殖，再接种至MS、1/2MS培养基中进行生根诱导，筛选出最佳生根培养基。结果表明:最佳增殖芽培养基分别为:达斯、弗州 MS+2.0 mg·L-16-BA +0.1 mg·L-1 NAA；丰香 MS+0.5 mg·L-16-BA +0.05 mg·L-1NAA。1/2MS培养基是草莓的最佳生根的基本培养基；其中在1/2MS +0.5 mg·L-1IBA上试管苗生根率达100%且长势强壮，根粗且多。3个材料试管苗离体愈伤组织诱导率均较高，为高产优质草莓生产提供了依据。
- Abstract:
- Using Darth,Virginia,Fengxiang three varieties of strawberries as test materials，strawberry stem tip regeneration in vitro was researched to produce high-yielding and high-quality strawberries and to improve efficiency.With MS as the basic culture medium,different concentration gradient plant growth regulators were added to conduct buds induction and tube seedlings proliferation.Vaccination in MS,1/2 MS culture to rooting induction and picked out the best rooting medium.The results showed that:the best bud culture medium for proliferation respectively:Darth,Virginia MS + 2.0 mg·L-16-BA + 0.1 mg·L-1 NAA;Fengxiang MS + 0.5 mg·L-16-BA + 0.05 mg·L-1NAA.1/2 MS culture was the best rooting basic culture medium;Which in a 1/2 MS + 0.5 mg·L-1IBA,rooting rate was 100% and grew strong,stout and more.Three materials vitro-preservation callus induction were higher and provided the basis for the high yield and good quality strawberry production
参考文献/References:
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备注/Memo
基金项目：安徽省科技计划资助项目(1104a0303042)；安徽高校省级自然科学研究规划资助项目（KJ2011B126）；阜阳师范学院科技成果孵化基金资助项目（2011kjfh04）；阜阳师范学院教研资助项目（2008JXXM27）