[1]宋维富,赵海滨,张延滨,等.龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基（LGW-GS）鉴定[J].黑龙江农业科学,2012,(02):1-5.
　SONG Wei-fu,ZHAO Hai-bin,ZHANG Yan-bin,et al.LMW-GS Identification at Gli-A1/GluA3 Lociin NILs of Wheat Variety Longmai 19[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(02):1-5.
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龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基（LGW-GS）鉴定

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2012年02 页码: 1-5 栏目: 出版日期: 2012-02-10
Title:
LMW-GS Identification at Gli-A1/GluA3 Lociin NILs of Wheat Variety Longmai 19
文章编号:
1002-2767（2012）02-0001-05
作者:
宋维富1; 2; 赵海滨1; 2; 张延滨1; 2; 李集临1; 姜焕焕1; 2; 丁琦1; 2; 宋庆杰2
1.哈尔滨师范大学 生命科学与技术学院,黑龙江 哈尔滨150025；
2.黑龙江省农业科学院 作物育种研究所,黑龙江 哈尔滨150086
Author(s):
SONG Wei-fu1; 2; ZHAO Hai-bin1; 2; ZHANG Yan-bin1; 2; LI Ji-lin1; JIANG Huan-huan1; 2; DING Qi; SONG Qing-jie2
1.Life Science and Technology College of Harbin Normal University，Harbin，Heilongjiang 150025；
2.Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences，Harbin,Heilongjiang 150086
关键词:
低分子麦谷蛋白亚基; 近等基因系; STS标记
Keywords:
LMW-GS; NILs; STS marker
分类号:
S512；Q813
文献标志码:
A
摘要:
为了鉴定小麦品种龙麦19（L-19，2*，7+9，2+12）和一对L-19的Gli-A1位点醇溶蛋白近等基因系L-19-626（2*，7+9，5+10）与L-19-613（2*，7+9，5+10）中与Gli-A1位点紧密连锁的Glu-A3位点低分子麦谷蛋白亚基（LMW-GS），利用一套Glu-A3位点特异低分子麦谷蛋白亚基STS标记对L-19、L-19-613、L-19-626及L-19/L-19-626的F2个体进行PCR扩增。结果表明：在具有醇溶蛋白Gli-A1m的个体中（L-19-626类型），Glu-A3e反应均为阳性，在不具有醇溶蛋白Gli-A1m的个体中（L-19和L-19-613类型），Glu-A3c反应均为阳性。说明编码L-19-626醇溶蛋白的Gli-A1m基因与编码LMW-GS的Glu-A3e基因紧密连锁，编码L-19和L-19-613的Gli-A1位点醇溶蛋白基因（未命名）与编码LMW-GS的Glu-A3c基因紧密连锁。因此，L-19-626与L-19-613近等基因系品质间的差别很可能是由其LMW-GS Glu-A3e与Glu-A3c基因间的差异引起的。
Abstract:
In order to identify LMW-GS at Glu-A3 locus linked with Gli-A1 locus in common wheat variety Longmai 19(L-19,HMW-GS 2*,7+9,2+12)and a pair of NILs(L-19-613 and L-19-626)at Gli-A1 locus with genetic background of Longmai 19 with HMW-GS 5+10,the materials that L-19,L-19-626 and L-19-613,F2 progeny of L-19/L-19-626 were assayed by a set STS markers of LMW-GS at Glu-A3 locus.The results showed that Glu-A3e amplified specific band in L-19-626 type materials with gliadin Gli-A1m.in L-19 and L-19-613 type materials without gliadin Gli-A1m,Glu-A3c amplified specific band.These results indicated the Gli-A1m at Gli-A1 locus was linked with LGW-GS Glu-A3e at Glu-A3 locus and the undesignated gliadin at Gli-A1 locus in L-19-613 and L-19 were linked with LGW-GS Glu-A3c at Glu-A3 locus.It was possible that quality difference between NILs L-19-626 and L-19-613 was due to LMW-GS gene difference of Glu-A3e and Glu-A3c at Glu-A3 locus.

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备注/Memo

基金项目：国家自然科学基金资助项目（30871525）

第一作者简介：宋维富（1982-），男，黑龙江省甘南县人，在读博士，从事小麦遗传育种研究。E-mail:songweifu1121@126.com。
共同第一作者赵海滨（1974-），男，在读博士。
通讯作者：张延滨（1957-），男，江苏省仪征市人，硕士，研究员，从事小麦品质遗传及育种研究。E-mail:yanbin_zhang@163.com。

更新日期/Last Update: 2014-10-25