[1]李卫东,崔清梅,张 强,等.利用ISSR标记对恩施州茶树种质资源亲缘关系鉴定的研究[J].黑龙江农业科学,2011,(12):7-9.
　LI Wei-dong,CUI Qing-mei,ZHANG Qiang,et al.Identification of Tea Germplasm Genetic Relationshipin Enshi by ISSR Markers[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(12):7-9.
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利用ISSR标记对恩施州茶树种质资源亲缘关系鉴定的研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2011年12 页码: 7-9 栏目: 出版日期: 2011-12-10
Title:
Identification of Tea Germplasm Genetic Relationshipin Enshi by ISSR Markers
文章编号:
1002-2767（2011）12-0007-03
作者:
李卫东; 崔清梅; 张 强; 侯伟华; 袁程晓; 梁金波; 戴居会
恩施州农业科学院，湖北 恩施 445000
Author(s):
LI Wei-dong; CUI Qing-mei; ZHANG Qiang; HOU Wei-hua; YUAN Cheng-xiao; LIANG Jin-bo; DAI Ju-hui
Enshi Academy of Agricultural Sciences，Enshi，Hubei 445000
关键词:
恩施茶树; 种质资源; ISSR; 亲缘关系
Keywords:
Enshi tea; germplasm resources; ISSR; genetic relationship
分类号:
S571.1
文献标志码:
A
摘要:
为更好地收集、保存和研究茶树品种资源，从2008年开始应用ISSR分子标记技术，对恩施州茶树资源的亲缘关系和遗传多样性进行了研究。结果表明：采用CTAB法提取DNA，探索出茶树PCR扩增效果较好的反应体系及反应程序，筛选出28条左右多态性较高的ISSR引物，用类平均法进行聚类分析，得出供试品种遗传关系树状图。基于遗传相似系数的UPGMA聚类图中，在遗传相似性系数为0.64处，183份茶树种质资源被划分为两大类。
Abstract:
For the purpose of collecting,conserving and researching tea germplasm,the relationship and genetic diversity of Enshi local tea germplasm have been studied since 2008 by ISSR markers.The results showed that reaction system and response procedures with good PCR amplification effect of the tea tree were explored using CTAB law to extract DNA,28 about higher ISSR polymorphism primer were selected,the genetic relationship phylogenetic tree of the tested varieties was obtained by an average of clustering analysis method.The results showed that based on the genetic similarity coefficient groups by UPGMA cluster figure,at the genetic similarity coefficient was 0.64 place,183 tea tree germplasm resources were divided into two categories.

参考文献/References:

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[2]侯渝嘉,何桥,李中林,等.应用ISSR分子标记对茶树种质资源进行分子鉴定[J].西南农业学报,2007,20（6）:1272-1276.

[3]唐玉海，郭春芳，张木清,等.ISSR标记在茶树品种遗传多态性研究中的应用[J］.福建农林大学学报(自然科学版),2007,36(1):51-55.

[4]郭春芳,唐玉海,孙云,等.茶树种质资源遗传多样性的ISSR分析[J］.热带作物学报，2008，29（2）：181-186.

[5]Zietkiewicz E，Rafalski A，Labuda D．Genome fingerprinting by simple sequence repeat(SSR)2anchored polymerase chain reaction amplification[J]．Genomics，1994，20(2)：176-183.

[6]孙亮，陈大明，高其康，等.茶树基因组DNA提纯与鉴定[J］.茶叶科学，1997，17（2）：177-181.


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备注/Memo

基金项目： 湖北省农业科技创新中心资助项目（2007-620-001-03）

第一作者简介： 李卫东（1961-），男，湖北省恩施市人，高级农艺师，从事茶树育种栽培研究。

更新日期/Last Update: 2014-11-02