CHEN Ai-xiang.Fermentation Conditions of Antagonistic StrainXL12 against Alternaria alternate[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(09):42-44.
细链格孢菌拮抗细菌枯草芽孢杆菌XL12菌株发酵产抗菌蛋白条件研究
- Title:
- Fermentation Conditions of Antagonistic StrainXL12 against Alternaria alternate
- 文章编号:
- 1002-2767（2011）09-0042-03
- 关键词:
- 细链格孢菌; 拮抗细菌; 枯草芽孢杆菌XL12菌株; 抗菌蛋白; 发酵优化
- Keywords:
- Alternaria alternate; antagonistic bacteria; strain XL12 of Bacillus subtilis; antifungal protein; fermentation optimization
- 分类号:
- S432.9
- 文献标志码:
- A
- 摘要:
- 为了提高细链格孢菌[Alternaria alternate (Fr）Keissler］拮抗细菌枯草芽孢杆菌（Bacillus subtilis）XL12菌株的发酵产抗菌蛋白量，采用单因素法对XL12菌株的培养条件进行了优化，最终确定XL12最适宜的发酵条件为：基础培养基碳源和氮源(摩尔比）的比例为30∶1，接种量为0.5％，配制后灭菌前，培养液的pH调节在6.95～7.05，发酵温度为30～35℃，220 r·min-1摇床振荡培养72 h，用70%硫酸铵对XL12菌株发酵液上清液进行盐析，可以沉淀最多的抗菌蛋白。
- Abstract:
- In order to increase the quantity of antifungal protein from Bacillus subtilisXL12，the strain was cultured under different conditions.The optimization of fermentation factors,such as the C/N proportion of basic medium,inoculation amount,initial pH,fermentation temperature and fermentation time,was made by single factor experiments.The result showed that the optimal fermentation condition was initial pH 6.95～7.05 and shaking flask in 220 r·min-1speed,fermentation temperature was 30～35℃ for 72 hours，premise of which the C/N ration is 30∶1,inoculation amount is 0.5%,under the conditions,the most quantity of antifungal protein was obtained by the 70%(NH4）2SO4salting out supernatant fluid of strain XL12 fermentation.
参考文献/References:
[1]王军，辛玉成.冬枣黑斑病病原的分离与鉴定[J].青岛农业大学学报，2007,24(1):21-23. [2]王绪芬.提高冬枣产量和品质的关键技术[J].北方园艺,2008(9):91-92. [3]常慧红，刘俊展，张路生.冬枣病害的发生及防治措施[J].植物保护，2011（2）：159-160. [4]牛赡光，王清海，刘幸红,等.三种拮抗放线菌对冬枣黑斑病防治效果的研究[J].山东林业科技，2007(4):36-37. [5]王军，陈爱香，辛玉成.枯草芽孢杆菌XL12发酵液蛋白对冬枣黑斑病病原的抑制效果[J].贵州农业科学，2011，39（2）:114-116.
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备注/Memo
作者简介：陈爱香（1978-），女，山东省青岛市人，硕士，副教授，从事植物保护教学和科研工作。E-mail:aixiangchen03@163.com。