MA Xuena,YU Tao,ZHANG Jianguo,et al.Physiological Response of Different Low Temperature Tolerant Maize Inbred Lines to Low Temperature Stress During Seedling Stage[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(05):17-22.[doi:10.11942/j.issn1002-2767.2025.05.0017]
不同低温耐性玉米自交系苗期对低温胁迫的生理响应
- Title:
- Physiological Response of Different Low Temperature Tolerant Maize Inbred Lines to Low Temperature Stress During Seedling Stage
- 文章编号:
- 3
- Keywords:
- maize; low temperature stress; seedling stage; physiological response
- 文献标志码:
- A
- 摘要:
- 为探究玉米耐低温的生理机制，挑选了耐低温的玉米自交系B144和低温敏感的玉米自交系Q319作为试验材料，对其进行5 ℃的低温胁迫处理，通过比较玉米幼苗株高、生物量、SOD、POD、丙二醛含量、可溶性糖含量、根系活力以及叶绿素含量的变化，研究低温对不同基因型玉米幼苗形态的影响及其生理响应机制。结果表明，低温胁迫抑制了幼苗生长和生物量的积累，B144和Q319的平均株高分别下降了31.33%和34.42%。在低温胁迫条件下，与耐低温玉米自交系B144相比，低温敏感玉米自交系Q319的SOD活性、POD活性、可溶性糖含量、根系活力和叶绿素含量显著降低，但丙二醛含量在不同基因型玉米幼苗间呈增长趋势。表明，B144表现出较强的耐冷性，可溶性糖含量和多种抗氧化酶的共同参与是玉米幼苗耐冷性形成的响应机制。
- Abstract:
- In order to explore the physiological mechanism of low temperature tolerance in maize, the low-temperature-tolerant maize inbred line B144 and the low-temperature-sensitive maize inbred line Q319 were used as experimental materials, and treated in low-temperature stress at 5 ℃. By comparing the changes in plant height, biomass, SOD, POD, MDA activities, soluble sugar content, root vigour and chlorophyll content of maize during seedling stage were studied under low temperature (5 ℃), the effect of low temperatures on maize seedlings of different genotypes were studied. The results showed that, low temperature could significantly inhibit seedling growth and biomass accumulation, the average plant heights of B144 and Q319 decreased by 31.33% and 34.42% respectively. SOD activity, POD activity, soluble sugar content, root vigour and chlorophyll content of cold sensitive inbred lines Q319 decreased significantly compared to the tolerant maize inbred lines B144 under low-temperature stress conditions. However, malondialdehyde content showed an increasing trend among the seedlings of maize with different genotypes. The study showed that B144 exhibited strong cold tolerance, the accumulation of soluble sugar content and a variety of antioxidant enzymes jointly involved as the response mechanism of the formation of chilling resistance of maize seedlings.
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备注/Memo
收稿日期：2025-02-14 基金项目：黑龙江省科研业务费项目（CZKYF2025-1-B002）；黑龙江省农业科学院创新工程资助项目（CX23ZD05，CX23JQ04）；黑龙江省种业创新发展项目（2024HZYCXNK07）。 第一作者：马雪娜（1992-），女，硕士，研究实习员，从事玉米抗逆分子育种研究。E-mail：xuena19920207@163.com。 通信作者：于滔（1982-），女，博士，副研究员，从事玉米抗逆分子育种研究。E-mail：yutaoweiwei@163.com。