[1]白洁霞,李佳昕,刘璐璐,等.藏桃AmArg1基因克隆与互作蛋白筛选及抗逆性分析[J].黑龙江农业科学,2025,(01):40-50.[doi:10.11942/j.issn1002-2767.2025.01.0040]
　BAI Jiexia,LI Jiaxin,LIU Lulu,et al.Gene Cloning,Interacting Protein Screening and Stress Resistance Analysis of AmArg1 Gene from Amygdalus mira Koehne[J].HEILONGJIANG AGRICULTURAL SCIENCES,2025,(01):40-50.[doi:10.11942/j.issn1002-2767.2025.01.0040]
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藏桃AmArg1基因克隆与互作蛋白筛选及抗逆性分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2025年01 页码: 40-50 栏目: 出版日期: 2025-01-10
Title:
Gene Cloning,Interacting Protein Screening and Stress Resistance Analysis of AmArg1 Gene from Amygdalus mira Koehne
文章编号:
7
作者:
白洁霞; 李佳昕; 刘璐璐; 罗秋香
（东北盐碱植被恢复与重建教育部重点实验室/东北林业大学 生命科学学院，黑龙江 哈尔滨150040)
Author(s):
BAI Jiexia;  LI Jiaxin;  LIU Lulu;  LUO Qiuxiang
(Key Laboratory of Northeast Saline-alkali Vegetation Restoration and Reconstruction, Ministry of Education/School of Life Sciences, Northeast Forestry University, Harbin 150040，China)
关键词:
藏桃; 互作蛋白; 非生物胁迫; AmArg1基因
Keywords:
Amygdalus mira Koehne;  interacting proteins;  abiotic stress;  AmArg1 gene
DOI:
10.11942/j.issn1002-2767.2025.01.0040
文献标志码:
A
摘要:
为探究藏桃精氨酸酶1的耐逆性生物学功能，克隆了精氨酸酶1基因（AmArg1）全长ORF，用生物信息学技术分析其蛋白结构和理化性质，结果表明，该蛋白是酸性的无信号肽结构的非分泌蛋白。将AmArg1转入酵母细胞，其对酵母细胞无毒性、无自激活活性。通过酵母双杂交技术筛选出AmArg1的互作蛋白AmLhcb1、AmRabB1b 并在洋葱活体细胞内验证互作，并初步探究AmArg1在NaCl、甘露醇和CuSO4胁迫下的抗逆性反应，发现过表达转AmArg1酵母菌具有较强的胁迫耐受性。
Abstract:
In order to investigate the arginase 1 （AmArg1） of Amygdalus mira, the full-length ORF of the arginase 1，AmArg1was cloned and therefore, the deduced protein sequence was predicted for structure and physicochemical properties via bioinformatics analysis. The results showed that the protein was an acidic, non-secreted protein with no signal peptide structure.AmArg1 was transferred into yeast cells and it was non-toxic and non-self-activating for yeast cells. The interacting proteins, AmLhcb1 and AmRabB1b of AmArg1 were screened by yeast two hybrid assays, and their interactions were validated by BiFC analysis. Transgenic yeast in response to NaCl, mannitol, and CuSO4 stress was preliminarily explored, and found that over-expression of AmArg1 might increase the tolerance to these stresses.

备注/Memo

收稿日期：2024-07-23基金项目：国家自然科学基金(31500317)。

第一作者：白洁霞(1999-)，女，硕士研究生，从事植物逆境生理生态学研究。E-mail：b17735744603@163.com。
通信作者：罗秋香(1976-)，女，博士，副教授，从事植物逆境生理生态学研究。E-mail：luoqiuxiang@sina.com。

更新日期/Last Update: 2025-01-10