WANG Xuesong,ZHANG Yutong,YANG Wanting,et al.Synergistic Inhibitory Effects of Buckwheat and Oat Polyphenol Extracts on Colon Cancer Caco-2 Cell Proliferation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(10):61-68.[doi:10.11942/j.issn1002-2767.2024.10.0061]
荞麦和燕麦多酚提取物对结肠癌Caco-2细胞增殖的协同抑制作用
- Title:
- Synergistic Inhibitory Effects of Buckwheat and Oat Polyphenol Extracts on Colon Cancer Caco-2 Cell Proliferation
- 文章编号:
- 10
- Keywords:
- buckwheat; oatmeal; polyphenols; colon cancer; synergistic inhibitor
- 文献标志码:
- A
- 摘要:
- 为减少癌细胞对药物的抗药性，通过液质联用法、MTT法和流式细胞仪分析法对荞麦和燕麦多酚提取物进行组分鉴定以及对结肠癌Caco-2细胞增殖的协同抑制作用进行研究，并结合高效液相色谱-质谱鉴定(-)-表阿夫儿茶精-表儿茶素等10种化合物。结果表明，荞麦和燕麦多酚提取物对结肠癌Caco-2细胞抑制作用表现为一定的浓度范围内随着多酚提取物浓度的增加而极显著增加（P<0.01），其中，燕麦多酚提取物的抑制作用显著高于荞麦（P<0.05），最佳培养时间皆为24 h。联合作用条件下，燕麦浓度增加至6.0 mg·mL-1以上时，荞麦和燕麦多酚提取物呈现出协同抑制作用，其最佳浓度组合为荞麦（2.0或3.0 mg·mL-1）和燕麦（10.0 mg·mL-1），对结肠癌Caco-2细胞增殖抑制率浓度分别为47.03%和50.10%。经凋亡图谱分析发现，荞麦和燕麦组（C和D）不仅远远高于对照组，与同剂量燕麦组（B）相比，细胞凋亡率分别增加了3.5%和6.8%。因此，说明荞麦和燕麦中多酚化合物存在形式之间的互补可有效地诱导细胞发生凋亡，且能够对多酚癌细胞增殖协同抑制作用提供帮助。
- Abstract:
- In order to reduce the drug resistance of cancer cells, the fractional identification of buckwheat and oat polyphenol extracts and their synergistic inhibition of colon cancer Caco-2 cell proliferation were investigated by liquid mass spectrometry, MTT and flow cytometry analysis. Ten compounds were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS), including (-)-epi-affeocatechin-epicatechin. The inhibitory effect of buckwheat and oat polyphenol extracts on colon cancer Caco-2 cells was shown to increase significantly (P<0.01) with increasing concentrations of polyphenol extracts in a range of concentrations, with the inhibitory effect of oat polyphenol extracts being significantly higher than that of buckwheat (P<0.05), and the optimal incubation time was 24 hours for both. Under the combined conditions, buckwheat and oat polyphenol extracts showed synergistic inhibition when the concentration of oats was increased above 6.0 mg·mL-1. The optimal combination of buckwheat (2.0 or 3.0 mg·mL-1) and oats (10.0 mg·mL-1) inhibited the proliferation of colon cancer Caco-2 cells by 47.03% and 50.10%, respectively. Analysis of the apoptosis profiles revealed that not only were the buckwheat and oat groups (C and D) much higher than the control group, the apoptosis rates increased by 3.5% and 6.8%, respectively, compared with the same dose of oats (B). The results suggest that complementarity between the forms of polyphenolic compounds present in buckwheat and oats can be effective in inducing apoptosis and will contribute to the synergistic inhibition of polyphenolic cancer cell proliferation.
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备注/Memo
收稿日期：2024-04-18