FENG Yinghui,YU Jinli,LIU Jinwen.Mechanism of Leaf Color Change in Red Maple at Different Development Stages[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(05):63-69.[doi:10.11942/j.issn1002-2767.2024.05.0063]
不同发育阶段红枫叶片呈色机制
- Title:
- Mechanism of Leaf Color Change in Red Maple at Different Development Stages
- 文章编号:
- 11
- Keywords:
- Acer palmatum Atropurpureum; anthocyanins; transcriptome; leaf color
- 文献标志码:
- A
- 摘要:
- 为了探究红枫叶色变化的生理和分子机制，以不同发育阶段叶色差异显著的3种叶片为材料，利用生物化学和转录组学方法对叶色变化相关的生理指标和差异基因进行检测和分析。结果表明，不同发育阶段红枫叶片由红色发育为绿色过程中，叶片中的叶绿素含量、叶绿素合成前体ALA与PBG含量和类胡萝卜素含量呈上升趋势，而花色素苷含量、总酚和类黄酮含量显著下降；转录组数据显示不同发育阶段的红枫叶片存在6 931个差异表达基因，花青素和类黄酮生物合成途径关键酶的编码基因CHS、ANS、CHI、HHT、F3′H、FLS、ART在红色叶片中上调表达，两个MYB转录因子基因（MYB2和MYB113）和两个蛋白激酶基因（TMK1和RUL1）在红色叶片中上调表达。本研究初步阐明了不同发育阶段红枫叶色差异的生理特征，鉴定了花青素合成途径相关的结构基因和调控基因，为进一步研究红枫叶色变化的分子机制提供了丰富的遗传信息。
- Abstract:
- In order to explore the physiological and molecular mechanisms of leaf color change in red maple, three color types of leaves with significant differences at different developmental stages were used as experimental materials and biochemical and transcriptome sequencing analysis were carried out to clarify and analyze physiological characteristics and differential genes related to leaf color changes. The results showed that the chlorophyll content, chlorophyll synthesis precursor ALA and PBG content, and carotenoid content significantly increased and anthocyanin content, total phenol and flavonoid content significantly decreased. Transcriptome analysis showed that there were 6 931 differentially expressed genes in red maple leaves at different developmental stages. The structural genes CHS, ANS, CHI, HHT, F3′H, FLS, and ART, which were key enzymes in the anthocyanin biosynthesis pathway, were highly expressed in the red leaves. Two gens of MYB transcription factors (MYB2 and MYB113) and two gens of protein kinases (TMK1 and RUL1) were highly expressed in the red leaves. The study preliminarily elucidated the physiological characteristics of leaf color differences and identified structural and regulatory genes related to anthocyanin synthesis pathways in red maple at different developmental stages, which will provide rich genetic information for further research on the molecular mechanisms of leaf color changes in red maple.
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备注/Memo
收稿日期：2023-12-18