PENG Yuwei,WANG Shanglong,WANG Yuanfeng,et al.Correlation Analysis of X Chromosome Multiple Embryonic Traits in Qira Black Sheep[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(03):39-45.[doi:10.11942/j.issn1002-2767.2024.03.0039]
策勒黑羊X染色体多胎性状的关联分析
- Title:
- Correlation Analysis of X Chromosome Multiple Embryonic Traits in Qira Black Sheep
- 文章编号:
- 7
- 文献标志码:
- A
- 摘要:
- 为促进多胎性状策勒黑羊品种的培育，以策勒黑羊多胎性状和X染色体分子标记为对象，探究策勒黑羊多胎性状在X染色体上的遗传解析。选择100只策勒黑羊进行基因分型，利用PLINKv1.90进行质量控制，同时对X染色体上的SNP位点和策勒黑羊的多胎性状进行关联分析，选取P＜0.01的SNP位点进行标记，将筛选出的SNP位点附近的基因进行注释和分析，获得X染色体多胎性的候选基因。结果表明,有14个SNPs与策勒黑羊X染色体多胎性状在全基因组范围内显著相关。共筛选出20个候选基因，对20个候选基因进行生物学功能分析，多胎性状相关联的候选基因6个，其中，FTH1基因已有文献报道是多胎性状的候选基因。
- Abstract:
- In order to breed the Qira black sheep breed for multiple birth traits, the genetic resolution of multiple birth traits on the X chromosome of Qira black sheep was investigated by using multiple birth traits and X chromosome molecular markers. One hundred Qira black sheep were selected for genotyping, and PLINKv1.90 was used for quality control. At the same time, SNP loci on the X chromosome and multiparity trait of Qira black sheep were analyzed by association, and SNP loci with P＜0.01 were selected for labeling, and genes near the screened SNP loci were annotated and analyzed, so as to obtain the candidate genes for multiparity on the X chromosome. The results showed that 14 SNPs were significantly associated with X chromosome multiparity in Qira black sheep on a genome-wide scale. A total of 20 candidate genes were screened and analyzed for their biological functions. Six candidate genes were associated with the multiple birth trait, among which, the FTH1 gene has been reported in the literature as a candidate gene for the multiple birth trait.
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备注/Memo
收稿日期：2023-11-27