YAN Rong,LIU Tongtong,HUANG Di,et al.Response of BoNR8 lncRNA to ABA Stress and Its Regulation of Hypocotyl Growth in Brassica oleracea[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(07):59-63.[doi:10.11942/j.issn1002-2767.2023.07.0059]
结球甘蓝BoNR8 lncRNA对ABA胁迫响应及对下胚轴生长的调节作用
- Title:
- Response of BoNR8 lncRNA to ABA Stress and Its Regulation of Hypocotyl Growth in Brassica oleracea
- 文章编号:
- 11
- Keywords:
- long noncoding RNA; Brassica oleracea; hypocotyl; ABA stress
- 文献标志码:
- A
- 摘要:
- 为探明结球甘蓝中RNA聚合酶III转录的非编码RNA BoNR8 lncRNA在ABA应激条件下的反应以及对下胚轴生长及相关基因表达的影响。利用生物信息学分析发现，BoNR8上游启动子区域存在ABA响应元件。预测其可能响应ABA胁迫，因此用ABA处理正常生长3 d 的结球甘蓝种子，发现ABA显著诱导BoNR8 lncRNA表达；随后利用qRT-PCR的方法对BoNR8过表达拟南芥突变体进行相关功能分析发现，正常条件下，拟南芥中BoNR8 lncRNA过量表达抑制了下胚轴生长；ABA应激条件下，BoNR8 lncRNA过表达促进下胚轴生长及下胚轴生长相关基因EXP3和PIN7表达。这表明，BoNR8 lncRNA可能通过响应ABA信号、影响下胚轴生长相关基因的表达，调节拟南芥下胚轴生长。
- Abstract:
- In order to investigate the response of noncoding RNA BoNR8 lncRNA transcribed by RNA polymerase III in Brassica oleracea under ABA stress and its effects on hypocotyl growth and related gene expression. In this study， it was found by bioinformatics analysis that ABA response elements existed in the upstream promoter region of BoNR8. It is predicted that it may respond to ABA stress， so the normal growth of Brassica oleracea seeds for 3 days was treated with ABA， and it was found that ABA significantly induced the expression of BoNR8 lncRNA; Subsequently， qRT-PCR was used to analyze the related functions of BoNR8 overexpressed Arabidopsis mutant. It was found that under normal conditions， the overexpression of BoNR8 lncRNA in Arabidopsis inhibited the growth of hypocotyls. Under ABA stress， the overexpression of BoNR8 lncRNA promoted hypocotyl growth and the expression of related genes EXP3 and PIN7. This indicates that BoNR8 lncRNA may regulate the growth of Arabidopsis hypocotyl by responding to ABA signals and affecting the expression of genes related to hypocotyl growth.
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备注/Memo
收稿日期：2023-02-10