WANG Tong,ZHOU Chenni,LI Tao,et al.Research Progress on Extraction Technology of Protein and β-Glucan in Highland Barley and Oat[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(04):95-100.[doi:10.11942/j.issn1002-2767.2023.04.0095]
青稞与燕麦蛋白质及β-葡聚糖提取工艺的研究进展
- Title:
- Research Progress on Extraction Technology of Protein and β-Glucan in Highland Barley and Oat
- 文章编号:
- 19
- Keywords:
- highland barley; oat; protein; β-glucan; extraction process
- 文献标志码:
- A
- 摘要:
- 青稞与燕麦中蛋白质及β-葡聚糖的提取工艺主要有碱法、酶法、复合法,本文主要对碱法、酶法以及物理方法辅助其他提取方法进行论述。综合得出,虽然碱法适合提取植物蛋白质,但由于其高浓度碱会使植物蛋白产生反应,使其营养物质变性,进而产生不利于肾脏功能的有毒物质。相对于碱法,酶法反应条件温和且不会产生有毒物质,但其成本较高。此外,在上述提取工艺过程中加入物理法进行辅助不仅可以降低提取成本,而且可显著提升植物蛋白提取效率。
- Abstract:
- The extraction technologies of protein and β-glucan in highland barley and oat mainly include alkaline method, enzymatic method and composite method. This article mainly discussed the alkali method, enzymatic method and physical method and their assisting other extraction methods. It is comprehensively concluded that although alkali method was suitable for extracting plant protein, its high concentration of alkali will affect the plant protein, making its nutrients denatured and destroyed, and will produce toxic substances that damage kidney function. Compared with alkaline process, enzymatic reaction conditions were mild and do not produce toxic substances, but its cost was high. The addition of physical method in the extraction process can not only reduce the extraction cost, but also significantly improve the efficiency of plant protein extraction.
参考文献/References:
[1]杨春艳,沈渭寿,王涛.近30年西藏耕地面积时空变化特征[J].农业工程学报,2015,31(1):264-271.[2]张毅,马跃峰,贠民政,等.近30年西藏地区耕地面积及主要农作物时空变化特征[J].高原农业,2020,4(1):17-25.[3]谭占坤,商振达,刘锁珠,等.西藏主要饲料粮调查及养分测定分析[J].饲料研究,2020,43(6):91-96.[4]罗静,李玉锋,胥霞.青稞中的活性物质及功能研究进展[J].食品与发酵工业,2018,44(9):300-304.[5]姚豪颖叶,聂少平,鄢为唯,等.不同产地青稞原料中的营养成分分析[J].南昌大学学报(工科版),2015,37(1):11-15.[6]王鹏珍,牛忠海,张世满,等.青稞原料营养成分浅析[J].酿酒科技,1997(3):30-31.[7]洛桑旦达,强小林.青稞特有营养成份分析与开发利用现状调查研究报告[J].西藏科技,2001(8):55-64,54.[8]IZYDORCZYK M S,STORSLEY J,Labossiere D,et al.Variation in total and soluble β-glucan content in hulless barley:effects of thermal,physical,and enzymic treatments[J].Journal of Agricultural and Food Chemistry,2000,48(4):982-989.[9]徐菲,党斌,杨希娟,等.不同青稞品种的营养品质评价[J].麦类作物学报,2016,36(9):1249-1257.[10](徐冬勤,张晓航,戚春建.β-葡聚糖在抗肿瘤免疫应答中的研究进展[J].中国免疫学杂志,2016,32(11):1715-1717.[11]AGOSTINI S,CHIAVACCI E,MATTEUCCI M,et al.Barley beta-glucan promotes MnSOD expression and enhances angiogenesis under oxidative microenvironment[J].Journal of Cellular and Molecular Medicine,2015,19(1):227-238.[12]MCINTOSH G H,NEWMAN R K,NEWMAN C W.Barley foods and their influence on cholesterol metabolism[J].Plants in Human Nutrition,1995,77:89-108.[13]DAVIDSON M H,DUGAN L D,BURNS J H,et al.The hypocholesterolemic effects of β-glucan in oatmeal and oat bran:a dose-controlled study[J].The Journal of the American Medical Association,1991,265(14):1833-1839.[14]WOOD P J,BEER M U,BUTLER G.Evaluation of role of concentration and molecular weight of oat β-glucan in determining effect of viscosity on plasma glucose and insulin following an oral glucose load[J].British Journal of Nutrition,2000,84(1):19-23.[15]HALLFRISCH J,SCHOLFIELD D J,BEHALL K M.Diets containing soluble oat extracts improve glucose and insulin responses of moderately hypercholesterolemic men and women[J].The American Journal of Clinical Nutrition,1995,61(2):379-384.[16]TAPPY L,GGOLZ E,WRSCH P.Effects of breakfast cereals containing various amounts of β-glucan fibers on plasma glucose and insulin responses in NIDDM subjects[J].Diabetes Care,1996,19(8):831-834.[17]OU S,KWOK K,LI Y,et al.In vitro study of possible role of dietary fiber in lowering postprandial serum glucose[J].Journal of Agricultural and Food Chemistry,2001,49(2):1026-1029.[18]JENKINS A L,JENKINS D J A,ZDRAVKOVIC U,et al.Depression of the glycemic index by high levels of β-glucan fiber in two functional foods tested in type 2 diabetes[J].European Journal of Clinical Nutrition,2002,56(7):622-628.[19]章海燕,张晖,王立,等.燕麦研究进展[J].粮食与油脂,2009(8):7-9.[20]MOHAMED A,BIRESAW G,XU J,et al.Oats protein isolate:thermal,rheological,surface and functional properties[J].Food Research International,2008,42(1):107-114.[21]许英一,王宇,林巍.酶法提取燕麦蛋白理化性质研究[J].食品工业,2018,39(7):72-75.[22]徐悦,郭亚男,李顺秀,等.超声对燕麦蛋白氧化聚集体结构及特性的影响[J].食品工业科技,2020,41(11):85-91,116.[23]YUE J X,GU Z X,ZHU Z B,et al.Impact of defatting treatment and oat varieties on structural,functional properties,and aromatic profile of oat protein[J].Food Hydrocolloids,2021,112:106368.[24]谢昊宇,贾冬英,迟原龙,等.青稞蛋白质碱法提取条件的优化研究[J].食品工业科技,2014,35(18):281-283,287.[25]吴桂玲,刘立品,李文浩,等.碱溶酸沉法提取青稞蛋白质的工艺研究[J].食品研究与开发,2015,36(5):19-24.[26]张文会,顿珠次仁.碱溶酸沉法提取青稞蛋白的工艺研究[J].食品科技,2014,39(10):219-222.[27]王金水,李涛,焦健.水酶法提取青稞蛋白工艺研究[J].食品工业科技,2010,31(9):267-269.[28]葛娜,易翠平,姚惠源.碱性蛋白酶提取大米水解蛋白的研究[J].粮食与饲料工业,2006(4):25-27.[29]葛娜,易翠平,姚惠源.酸性蛋白酶提取大米水解蛋白的研究[J].食品与机械,2006(1):53-55,58.[30]葛娜.酶法提取大米蛋白及其应用的研究[D].无锡:江南大学,2006.[31]奚海燕.大米蛋白的提取及改性研究[D].无锡:江南大学,2008.[32]萨如拉,文静,于田田,等.超声波辅助酶法提取燕麦麸皮蛋白质的工艺研究[J].赤峰学院学报(自然科学版),2021,37(1):17-20.[33]杨希娟,党斌,吴昆仑,等.青稞蛋白的超声波辅助提取工艺及其功能特性研究[J].中国食品学报,2013,13(6):48-56.[34]霍金杰,肖志刚,王娜,等.青稞蛋白质的微波辅助提取工艺及性质研究[J].食品研究与开发,2020,41(21):145-153.[35]杨进妹,王婷,何荣海.超声对酶解反应的影响[J].农产品加工,2010(7):70-71,73.[36]游茂兰,覃小丽,段娇娇,等.超声-微波协同提取青稞β-葡聚糖[J].食品与发酵工业,2019,45(8):178-183.[37]马国刚,王建中.超声波辅助提取青稞β-葡聚糖的工艺条件优化[J].食品科技,2009,34(11):168-174.[38]徐菲,党斌,迟德钊.超声波辅助提取青稞β-葡聚糖工艺优化[J].食品科技,2014,39(12):217-221.[39]连喜军,张平平,罗庆丰.西藏青稞中β-葡聚糖提取的研究[J].天津农学院学报,2005(4):25-27.[40]郝勇.青稞β-葡聚糖提取纯化工艺研究[D].兰州:兰州大学,2011.[41]罗燕平,李家林,张雪飞.微波辅助提取青稞β-葡聚糖工艺优化[J].农产品加工,2016(14):35-38.[42]王谦,董海丽.超高压提取青稞β-葡聚糖工艺优化[J].粮食与油脂,2016,29(5):79-81.[43]王谦,董海丽.青稞β-葡聚糖高压微波提取工艺条件的优化[J].轻工科技,2015,31(3):16-17,41.[44]邓爱华,杨品红,刘也嘉,等.青稞β-葡聚糖超声辅助提取工艺优化[J].粮食科技与经济,2022,47(3):108-112.[45]郭欢.青稞β-葡聚糖的提取分离、结构表征、化学修饰及其生物活性研究[D].成都:四川农业大学,2020.[46]TEMELLI F.Extraction and functional properties of barley β-glucan as affected by temperature and pH[J].Journal of Food Science,1997,62(6):1194-1201.[47]张峰.青稞β-葡聚糖的分离纯化及其理化性质研究[D].重庆:西南农业大学,2005.[48]顾飞燕.青稞β-葡聚糖的提取及其在化妆品中的应用[D].上海:上海应用技术大学,2018.[49]刘新琦,何先喆,刘洁纯,等.发酵法提取青稞麸皮中β-葡聚糖的工艺优化及其理化性质研究[J].食品工业科技,2020,41(7):49-54.[50]刘建垒,郝利平.影响碱提酸沉法提取燕麦蛋白因素的分析[J].山西农业大学学报(自然科学版),2013,33(5):429-435.[51]翟爱华,季娜,刘恒芝.燕麦分离蛋白提取工艺研究[J].食品科学,2006(12):439-441.[52]曹辉,李蕾,马海乐.燕麦分离蛋白提取工艺研究[J].安徽农业科学,2009,37(22):10681-10683.[53]李桂娟,樊守瑞,尤伟,等.燕麦蛋白的制备工艺研究[J].食品与机械,2009,25(1):120-123.[54]高兴,李桂娟.碱提酸沉法提取燕麦蛋白的工艺研究[J].中国西部科技,2010,9(31):29-31.[55]赵素斌,张晓平,任清.3种方法提取燕麦麸蛋白及其产物的比较[J].食品科学,2010,31(14):71-79.[56]刘光明,李林.碱提酸沉法制备燕麦浓缩蛋白的工艺研究[J].湖南文理学院学报(自然科学版),2011,23(1):60-62.[57]张晓斌.燕麦蛋白和淀粉的提取纯化工艺研究[J].粮油食品科技,2011,19(5):12-14,32.[58]张晓平,任清,蒋琼,等.酶法提取燕麦蛋白及脱色工艺的研究[J].食品工业科技,2009,30(6):268-270,274.[59]李洋,韩小贤,张杰,等.碱性蛋白酶法提取燕麦蛋白的研究[J].河南工业大学学报(自然科学版),2012,33(6):77-80.[60]吴素萍.超声辅助酶法提取燕麦蛋白的研究[J].粮食与饲料工业,2007(9):22-25.[61]刘建垒,李英杰,郝利平.均匀试验设计优化酶法提取燕麦全粉蛋白质研究[J].中国粮油学报,2013,28(8):78-82,86.[62]申瑞玲,董吉林,王章存.裸燕麦麸皮β-葡聚糖微波提取工艺研究[J].中国农学通报,2006(10):316-320.[63]汪海波,刘大川,谢笔钧.燕麦中β-葡聚糖的提取及分离纯化工艺研究[J].食品科学,2004(5):143-147.[64]潘妍.生物转化提取燕麦β-葡聚糖及其化妆品功效研究[D].北京:北京工商大学,2010.[65]张娟,杜先锋.二次回归法优化燕麦β-葡聚糖提取工艺的研究[J].中国食品添加剂,2006(5):81-85.[66]张玉良.燕麦β-葡聚糖的微生物法提取及理化性质研究[D].大连:大连工业大学,2012.[67]谢宇霞,吴家乾,黄玉,等.碱法提取米糠中粗蛋白的几个影响因素探讨[J].粮食加工,2022,47(3):34-36.[68]de GROOT A P,SLUMP P.Effects of severe alkali treatment of proteins on amino acid composition and nutritive value[J].The Journal of Nutrition,1969,98(1):45-56.[69]魏明英,邬应龙.大米蛋白的研究进展[J].粮食与饲料工业,2003(3):44-45.[70]王文高,陈正行,姚惠源.不同蛋白酶提取大米蛋白质的研究[J].粮食与饲料工业,2002(2):41-42.[71]李宏凯.酶法水解植物蛋白概况[J].中国食品添加剂,2010(4):233-237.[72]彭清辉,林亲录,陈亚泉.大米蛋白研究与利用概述[J].中国食物与营养,2008(8):34-36.[73]郭荣荣,潘思轶,王可兴.碱法与酶法提取大米蛋白工艺及功能特性比较研究[J].食品科学,2005(3):173-177.[74]席文博,赵思明,刘友明.大米蛋白分离提取的研究进展[J].粮食与饲料工业,2003(10):45-47.[75]许凤,王长远.响应面法优化物理辅助碱法提取米糠蛋白工艺[J].食品科学,2014,35(20):11-16.[76]张安宁,王晔,李照晴,等.反复冻融辅助弱碱法提取米糠蛋白[J].食品工业,2020,41(10):141-145.[77]袁孝瑞,陈贺宇,刘玉,等.超声波辅助热碱法提取藜麦蛋白的工艺优化[J].食品工业科技,2022,43(13):190-197.[78]吴佳,林向阳,黄迪惠,等.燕麦β-葡聚糖的冻融法提取及其结构表征[J].中国食品学报,2011,11(4):48-54.
相似文献/References:
[1]路 颖,陈 浩,杨 学,等.黑龙江省燕麦科研和生产现状及发展建议[J].黑龙江农业科学,2010,(02):125.
LU Ying,CHEN Hao,YANG Xue,et al.Scientific Research and Production Condition of Oat and Its Development Suggestions in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(04):125.
[2]陈 浩,路 颖,杨 学,等.黑龙江省燕麦栽培要点[J].黑龙江农业科学,2010,(02):138.
CHEN Hao,LU Ying,YANG Xue,et al.Outline of Oat Cultivation in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(04):138.
[3]盖颜欣,王艳芝,季志强,等.承德冷凉山区燕麦和荞麦种植结构及开发情况[J].黑龙江农业科学,2017,(09):23.[doi:10.11942/j.issn1002-2767.2017.09.0023]
GAI Yan-xin,WANG Yan-zhi,JI Zhi-qiang,et al.Plantation Structure and Developing Situation of Oats and Buckwheat Along the Dam Areas in Chengde City[J].HEILONGJIANG AGRICULTURAL SCIENCES,2017,(04):23.[doi:10.11942/j.issn1002-2767.2017.09.0023]
[4]姜莉莉.燕麦猴头菇保健鸡肉丸的研制[J].黑龙江农业科学,2019,(09):104.[doi:10.11942/j.issn1002-2767.2019.09.0104]
JIANG Li-li.Development of Healthy Chicken Meatball with Oat and Hericium erinaceus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2019,(04):104.[doi:10.11942/j.issn1002-2767.2019.09.0104]
[5]王艳芝,盖彦华,杨保峰,等.冀北冷凉山区燕麦播种量及施肥优化模式研究[J].黑龙江农业科学,2020,(06):34.[doi:10.11942/j.issn1002-2767.2020.06.0034]
[J].HEILONGJIANG AGRICULTURAL SCIENCES,2020,(04):34.[doi:10.11942/j.issn1002-2767.2020.06.0034]
[6]王雪松,张羽彤,杨婉婷,等.荞麦和燕麦多酚提取物对结肠癌Caco-2细胞增殖的协同抑制作用[J].黑龙江农业科学,2024,(10):61.[doi:10.11942/j.issn1002-2767.2024.10.0061]
WANG Xuesong,ZHANG Yutong,YANG Wanting,et al.Synergistic Inhibitory Effects of Buckwheat and Oat Polyphenol Extracts on Colon Cancer Caco-2 Cell Proliferation[J].HEILONGJIANG AGRICULTURAL SCIENCES,2024,(04):61.[doi:10.11942/j.issn1002-2767.2024.10.0061]
备注/Memo
收稿日期:2022-10-20