CUI Hualei,GUO Huanhuan,YANG Lixiao,et al.Leaf Morphology and Photosynthetic Characteristics Analysis of Diploid and Polyploid Ulmus pumila[J].HEILONGJIANG AGRICULTURAL SCIENCES,2023,(03):68-72.[doi:10.11942/j.issn1002-2767.2023.03.0068]
二倍体及多倍体白榆叶片形态与光合特性分析
- Title:
- Leaf Morphology and Photosynthetic Characteristics Analysis of Diploid and Polyploid Ulmus pumila
- 文章编号:
- 13
- 文献标志码:
- A
- 摘要:
- 白榆是我国重要的乡土树种之一。为促进我国多倍体白榆的人工杂交培育和选优,以二倍体、三倍体及同源四倍体白榆叶片为试材,测定叶片表型指标、显微差异及光合生理特性,比较分析不同倍体间的变异程度及基因倍性发生改变对白榆产生的影响。结果表明,三倍体及同源四倍体白榆叶长、叶宽、叶面积都显著高于二倍体白榆,可见白榆在叶长、叶宽、叶面积这些形态学特征方面表现出多倍化后的巨大化特征。通过显微镜观察发现四倍体及三倍体白榆叶片上下表皮厚度、栅栏组织厚度、海绵组织厚度这些指标都高于二倍体白榆,巨大化特征表现十分突出,置于相同放大倍数视野下观察,出现了倍性增加气孔器密度稀疏的现象,可见气孔器密度与倍性有明显的相关性。三倍体及同源四倍体白榆的净光合速率(Pn)和蒸腾速率(Tr)都显著高于二倍体白榆,说明倍性的增加会使白榆的Pn及Tr增加。白榆叶绿素a (Chl a)含量高于叶绿素b(Chl b)含量,并且Chl a、Chl b及总叶绿素(Chl)含量随着倍性的增加而增大,表现出明显的多倍体植株的光合色素含量增加的特征。
- Abstract:
- Ulmus pumila is one of the important native species in China. In order to promote artificial hybrid cultivation and selection of polyploid Ulmus pumila in our country. Using diploid, triploid and homologous tetraploid Ulmus pumila leaves as test materials,the phenotypic indexes, microscopic differences and photosynthetic physiological characteristics of leaves were measured .The degree of variation among different ploidy and the effect of gene ploidy change on Ulmus pumila were compared and analyzed. The results showed that the leaf length, leaf width and leaf area of triploid and homologous tetraploid Ulmus pumila were significantly higher than those of diploid Ulmus pumila. It can be seen that the morphological characteristics of Ulmus pumila such as leaf length, leaf width and leaf area showed magnification after multiploidy. The thickness of upper and lower epidermis, palisade tissue and spongy tissue of leaves of tetraploid and triploid Ulmus pumila were higher than those of diploid Ulmus pumila, and the characteristics of magnification were very prominent.Under the same magnification field, the ploidy increased the sparse density of stomatal apparatus, this showed there was obvious correlation between stomatal density and ploidy. The Pn and Tr of triploid and homologous tetraploid Ulmus pumila were significantly higher than those of diploid Ulmus pumila, so the increase of ploidy would increase the Pn and Tr of Ulmus pumila. The content of Chl a was higher than that of Chl b, and the contents of Chl a, Chl b and Chl increased with the increase of ploidy.It showed obvious characteristics of photosynthetic pigment content in polyploid plants.
参考文献/References:
[1]晓章.白榆[J].新农业,1984(16):39.[2]续九如,宋婉,邹受益,等.榆属树种遗传改良研究现状及思考[J].北京林业大学学报,2000(6):95-99.[3]王瑞娟.中华金叶榆种植及养护管理技术[J].农家参谋,2020(10):151.[4]刘道强,韩洪利.速生白榆树组培苗大田栽培技术[J].国土绿化,2016(9):53.[5]徐春梅.白榆树种的育苗及造林技术[J].农村实用科技信息,2014(8):13.[6]王书欣,朱延林,董铁民,等.白榆无性系主要经济性状的遗传分析[J].河南林业科技,1995(1):29-30.[7]张昭洁,郑亦津.山东榆科植物叶表皮结构的比较[J].山东大学学报(自然科学版),1990(3):375-382.[8]孙同兴.9种榆科植物叶表皮结构特征研究[J].亚热带植物科学,2008,37(4):1-8.[9]李贺敏,张江涛,高致明,等.中国榆属12种2变种木材解剖学研究[J].河南林业科技,2007(3):1-3,19.[10]杜久军,左力辉,梁海永,等.5种榆树光合特性对比[J].分子植物育种,2018,16(7):2348-2357.[11]王力源,李文杰,麻芸娇,等.白榆白化芽变突变体光合特性及转录组分析[J].西北林学院学报,2020,35(6):10-16.[12]曹德美,张亚红,成星奇,等.青杨不同种群叶片表型性状的遗传变异[J].林业科学,2021,57(8):56-67.[13]成星奇,贾会霞,孙佩,等.丹红杨×通辽1号杨杂交子代叶形性状的遗传变异分析[J].林业科学研究,2019,32(2):100-110.[14]罗向东,戴亮芳,钱春桃,等.黄瓜属不同倍性异源多倍体的形态及生理特性分析[J].西北植物学报,2008(3):542-546.[15]李文英,顾万春.蒙古栎天然群体表型多样性研究[J].林业科学,2005(1):49-56.[16]吕建珍,任莹,王宏勇,等.264份谷子主要育成品种(系)表型多样性综合评价[J].作物杂志,2022(4):22-31.[17]王长海,张晓艳,李金花.小叶杨与欧洲黑杨杂交子代苗期叶形变异分析[J].林业科学研究,2020,33(3):132-138.[18]周元元,王军,杨照剑,等.不同白木香种质叶表型遗传多样性分析[J].分子植物育种,2022,20(21):7281-7294.[19]张石虎,古敏,周玮,等.黄梁木多倍体离体诱导及其性状变异[J].分子植物育种,2022,20(7):2372-2383.[20]张源源,岳秦伊,濮寿琴,等.橡胶树四倍体来源鉴定及不同倍性叶片解剖结构比较[J].植物遗传资源学报,2019,20(6):1597-1605.[21]张源源,郭涵,袁红章,等.橡胶树不同倍性的气孔性状差异研究[J].热带作物学报,2017,38(3):389-394.[22]王冰,华佳文,李娅翔,等.不同绿化树种叶片叶绿素SPAD值对比研究[J].黑龙江农业科学,2015(11):111-114.[23]孙玉婷,王映龙,杨红云,等.RGB与HSI色彩空间下预测叶绿素相对含量的研究[J].浙江农业学报,2018,30(10):1782-1789.[24]CASAR,CASTALDI F,PASCUCCI S,et al.Chlorophyll estimation in field crops: an assessment of handheld leaf meters and spectral reflectance measurements[J].The Journal of Agricultural Science,2015,153(5):876-890.[25]李秀宇.四倍体刺槐苗期表型生理特性及再生能力的评价[D].北京:北京林业大学,2020.[26]张丞慧,李凯铭,赵琪,等.新疆野苹果多倍体诱导及叶片特征分析[J].天津农学院学报,2021,28(4):6-10.[27]吴丽杰,李甲斌,刘旭霞,等.苹果二倍体和三倍体几个生理指标的比较[J].河北果树,2002(1):4-5.[28]刁凯.不同枣树品种光合特性规律研究[D].乌鲁木齐:新疆农业大学,2016.
备注/Memo
收稿日期:2022-11-27