[1]周丽,张萍,牛春,等.筑波链霉菌诱变株的筛选与保藏方法研究[J].黑龙江农业科学,2022,(06):84-88.[doi:10.11942/j.issn1002-2767.2022.06.0084]
　ZHOU Li,ZHANG Ping,NIU Chun,et al.Breeding and Preservation Method of Mutant Strain of Streptomyces tsukubaensis[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(06):84-88.[doi:10.11942/j.issn1002-2767.2022.06.0084]
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筑波链霉菌诱变株的筛选与保藏方法研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年06 页码: 84-88 栏目: 出版日期: 2022-06-10
Title:
Breeding and Preservation Method of Mutant Strain of Streptomyces tsukubaensis
作者:
周丽; 张萍; 牛春; 石彦鹏
宁夏泰瑞制药股份有限公司，宁夏 银川 750002
Author(s):
ZHOU Li; ZHANG Ping; NIU Chun; SHI Yan-peng
Ningxia Tairui Pharmaceutical Limited Company,Yinchuan 750002，China
关键词:
常压室温等离子体技术; 他克莫司; 突变高产菌种; 保藏方式
Keywords:
atmospheric pressure and room temperature plasma technology;  tacrolimus;  mutant high-yield strain;  depository
DOI:
10.11942/j.issn1002-2767.2022.06.0084
文献标志码:
A
摘要:
为了获得能稳定遗传的他克莫司生产菌种，供生产使用，本研究采用常压室温等离子体(ARTP)技术对他克莫司出发菌株TKM20-1进行诱变筛选，并对菌株保藏方法进行比较。结果表明：筛选获得1株可稳定遗传的突变高产菌株TKM21-15，相较于出发菌株，摇瓶效价提高了20%；对突变菌株进行4种保藏方式研究，确定该菌种可采用沙土管保藏法、冻干管保藏法、甘油管低温保藏法和甘油乳糖管保藏法来保藏，且沙土管保藏法和冻干管保藏法更适用于该菌种。
Abstract:
In order to obtain a stable genetic strain of tacrolimus for large-scale production,in this study,the atmospheric pressure room temperature plasma(ARTP)technology was used to mutagenize and select the tacrolimus starting strain TKM20-1,and strain preservation methods were compared.The results showed that a stable hereditary mutant high yielding strain TKM21-15 was obtained.Compared with the starting strain,the shake flask titer was obtained，increased by 20%.The mutant strain was studied in four preservation methods.It was determined that this strain can be preserved in sand soil tubes,freeze-dried tubes,glycerol cryogenic tubes and glycerol-lactose tubes.However,the sand tube preservation method and the freeze-drying tube preservation method are more suitable for this strain.

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备注/Memo

收稿日期：2022-02-02

第一作者：周丽（1990-），女，硕士，从事生物制药研究。E-mail:1463465891@qq.com。

更新日期/Last Update: 2022-07-29