CHU Bo-yan,ZHAO Yu-fen,GUO Shu-ying,et al.Effects of Different Concentrations of Colchicine on Seed Germination and Seedling Growth of Euonymus bungeanus[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(05):64-68.[doi:10.11942/j.issn1002-2767.2022.05.0064]
不同浓度秋水仙素处理对丝棉木种子发芽和幼苗生长的影响
- Title:
- Effects of Different Concentrations of Colchicine on Seed Germination and Seedling Growth of Euonymus bungeanus
- Keywords:
- Euonymus bungeanus; colchicine; seed germination
- 文献标志码:
- A
- 摘要:
- 为促进丝棉木化学诱变技术研究,以丝棉木成熟种子及幼苗作为试材,以蒸馏水浸泡种子作为对照,采用0%、0.05%、0.10%、0.15%和0.20%浓度梯度的秋水仙素分别处理12,24,36和48 h,测定分析秋水仙素处理后丝棉木种子发芽、胚根生长指标及幼苗苗高、地径生长状况。结果表明:随着秋水仙素处理浓度的增加和处理时间的增长丝棉木种子的发芽率和出苗率显著降低,部分种子幼胚变短变粗,胚根生长量降低,幼苗生长受到抑制。秋水仙素低浓度(0.10%以下)处理12 h有利于丝棉木种子的萌发。
- Abstract:
- In order to promote the research of chemical mutagenesis technology of Euonymus bungeanus,the mature seeds and seedlings of Euonymus bungeanus were used as test materials,and the seeds soaked in distilled water were used as control.Colchicine with five different concentration gradients of 0%,0.05%,0.10%,0.15% and 0.20% were treated for 12,24,36 and 48 hours respectively.The seed germination,radicle growth index,seedling height and ground diameter growth of seedlings after colchicine treatment were measured and analyzed.The results showed that with the increase of colchicine treatment concentration and treatment time,the germination rate and seedling emergence rate of Euonymus bungeanus seeds decreased significantly,the young embryos of some seeds became shorter and thicker,the growth of radicle decreased,and the growth of seedlings was inhibited.The seeds treated with colchicine at low concentration (below 0.10%) for 12 h were conducive to seed germination.
参考文献/References:
[1]中国科学院中国植物志编委会.中国植物志[M]. 北京:科学出版社,1999.
[2]郑永平.丝棉木塑料营养袋育苗及荒山造林技术[J].山西林业科技,2020,49(3):45-46.
[3]全国林木种子标准化技术委员会.丝棉木播种育苗技术规程:LY/T 3271—2021[S].北京:中国标准出版社,2022.
[4]汪妮,张志轩. 低温及赤霉素处理丝棉木种子对其发芽力的影响[J]. 种子,2010,29(12):95-97.
[5]董东平,李燕飞.低温及赤霉素处理对丝棉木种子萌发的影响[J].许昌学院学报,2018,37(8):7-12.
[6]徐佳琦.秋水仙素对洋紫荆种子萌发及幼苗生长的影响[D].广州:华南农业大学, 2017.
[7]方庆.不同秋水仙素处理对石榴种子发芽及幼苗生长的影响[J].江苏农业科学,2019,47(19):142-144.
[8]包梅荣,李铁柱,乌云塔娜,等.秋水仙素处理油茶种子和幼苗变异的初步研究[J].内蒙古农业大学学报(自然科学版),2009(2):46-51.
[9]李辉玲,张建文.不同浓度秋水仙素处理对色素辣椒种子发芽及幼苗生长的影响[J].新疆农业科技,2021(5):19-21.
[10]任威威.秋水仙素对花生种子萌发和植株生长发育的影响[D].郑州:河南农业大学,2019.
[11]王丽艳,荆瑞勇,肖莉杰,等.扁茎黄芪离体快繁及多倍体诱导[J].草业学报,2009,18(1):94-99.
[12]程心旻,高山林,卞云云.白术同源四倍体的诱导和鉴定及其与二倍体过氧化物酶的比较[J].植物资源与环境学报,2003,12(1):16-20.
[13]胡瑞阳,段红静,吴博,等.秋水仙素处理杉木种子的诱变效应[J].西南林业大学学报,2013(6):7-12.
[14]鲍智娟,邢秀芹.秋水仙素处理甘草种子诱导多倍体的研究[J].吉林农业科技学院学报,2009,18(4):3-5.
相似文献/References:
[1]曹惠美,张月学,刘凤歧,等.秋水仙素对肇东苜蓿根尖细胞染色体的影响[J].黑龙江农业科学,2012,(05):121.
CAO Hui-mei,ZHANG Yue-xue,LIU Feng-qi,et al.Effect of Colchicine on Root Tip Chromosomein Zhaodong Alfalfa[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(05):121.
[2]杨 学.亚麻多倍体诱导技术研究[J].黑龙江农业科学,2002,(04):14.[doi:10.11942/j.issn1002-2767.2002.04.0014]
YANGXue.The Study on the Induction of Polyploid Flax[J].HEILONGJIANG AGRICULTURAL SCIENCES,2002,(05):14.[doi:10.11942/j.issn1002-2767.2002.04.0014]
备注/Memo
收稿日期:2022-01-11
基金项目:河北省科技厅重点研发计划项目(19226359D)。
第一作者:储博彦(1965-),女,学士,农业技术推广研究员,从事园林植物的育种与栽培技术研究。E-mail:chuboyan@163.com。