[1]顾晶,石彦鹏,牛春,等.癸酸钠抗性菌株产达托霉素的发酵补料策略[J].黑龙江农业科学,2022,(04):81-84.[doi:10.11942/j.issn1002-2767.2022.04.0081]
　GU Jing,SHI Yan-peng,NIU Chun,et al.Fermentation Feed Strategy for Daptomycin Production by Sodium Caprate-resistant Strains[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(04):81-84.[doi:10.11942/j.issn1002-2767.2022.04.0081]
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癸酸钠抗性菌株产达托霉素的发酵补料策略

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2022年04 页码: 81-84 栏目: 出版日期: 2022-04-10
Title:
Fermentation Feed Strategy for Daptomycin Production by Sodium Caprate-resistant Strains
作者:
顾晶; 石彦鹏; 牛春; 张萍
宁夏泰瑞制药股份有限公司,宁夏 银川 750101
Author(s):
GU Jing; SHI Yan-peng; NIU Chun; ZHANG Ping
Ningxiatairui Pharmaceutical Limited Company,Yinchuan 750101,China
关键词:
达托霉素; 玫瑰孢链霉菌; 癸酸钠; 正癸酸; 发酵瓶补料
Keywords:
daptomycin;  streptomyces roseosporus;  sodium caprate; N-decanoic acid; fermentation bottle refill
DOI:
10.11942/j.issn1002-2767.2022.04.0081
文献标志码:
A
摘要:
为提高达托霉素产生菌玫瑰孢链霉菌的发酵能力，通过筛选癸酸钠抗性菌株，优化其发酵瓶补加正癸酸的方法，提高达托霉素的摇瓶效价值。首先对原始菌株进行癸酸钠抗性选育，筛选出一株空白摇瓶效价值为43.4 mg·L-1的菌株TR21-29，利用该菌株对癸酸盐的耐受性，验证其发酵瓶补加正癸酸的最优方式。主要考察正癸酸的耦合剂，向发酵瓶添加正癸酸的补料时间、补料浓度和补料间隔，确定发酵过程中补加正癸酸的关键参数。结果表明：补料可将抗性菌株TR21-29的原始效价值由43.4 mg·L-1提高至138.1 mg·L-1，提高率为218.2%。补料方式为配制0.5 g·mL-1的正癸酸溶液，在发酵瓶培养24 h后开始补加，补料终浓度为1.5 mmol·L-1，补料间隔为4 h。
Abstract:
In order to improve the fermentation ability of daptomycin-producing bacterium streptomyces roseolae，by screening sodium caprate resistant strains，optimizing the method of supplementing N-decanoic acid in its fermentation flask，improving the shake flask effect value of tropicolin.The original strain was selected for sodium caprate resistance，a strain TR21-29 with a blank shake flask efficacy value of 43.4 mg·L-1 was screened out.We took advantage of the strain′s tolerance to caprate，verified the optimal strategy of supplementing N-decanoic acid in its fermentation flask.This study mainly investigated the coupling agent of N-decanoic acid,and feed time,feed concentration and feed interval for adding N-decanoic acid to fermentation flasks,determining the key parameters for the addition of N-decanoic acid to fermentation flasks.The results showed that the original efficacy value of the resistant strain TR21-29 was increased from 43.4 mg·L-1 to 138.1 mg·L-1，and the improvement rate was 218.2%.The feeding method is to start adding N-decanoic acid solution after culturing in the fermentation flask for 24 hours.The concentration of N-decanoic acid is 0.5 g·L-1，the feed final concentration is 1.5 mmol·L-1，and the feed is fed every 4 h

参考文献/References:

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备注/Memo

收稿日期：2021-11-26

第一作者：顾晶（1990-），女，学士，初级工程师，从事菌种发酵研究。E-mail：juw6883@dingtalk.com。

更新日期/Last Update: 2022-07-14