ZHANG Dong-mei,MU Lin-lin,QI Chun-jie,et al.SSR Analysis of Genetic Diversity of Amaranthus hypochondriacus Germplasm Resources[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(03):19-23.[doi:10.11942/j.issn1002-2767.2022.03.0019]
籽粒苋种质资源遗传多样性的SSR分析
- Title:
- SSR Analysis of Genetic Diversity of Amaranthus hypochondriacus Germplasm Resources
- 文献标志码:
- A
- 摘要:
- 为了解籽粒苋种质资源遗传多样性,本试验利用30对SSR引物对18份籽粒苋种质进行初步分析。结果表明:筛选出的21对SSR引物可扩增出清晰条带。试验共扩增出90个等位基因位点,其中81个为多态性位点,多态位点百分率(PPB)为90.00%。有效等位基因数(Ne)的变化范围在1.189 2~1.994 2之间;期望杂合度(He)的变幅介于0.159 1~0.498 5之间;Shannon′s信息指数(I)变幅为0.295 8~0.691 7;PIC值介于0.146 3~0.374 2之间,所选取的SSR标记具有中度多态水平。分子标记结果表明,筛选出来的引物可以很好地反映18种籽粒苋品系的遗传多样性,可以进一步进行籽粒苋种质资源的开发利用和品种选育。
- Abstract:
- In order to understand the genetic diversity of? A.hypochondriacus? resources,30 pairs of SSR primers were used to screen 18? A.hypochondriacus? varieties.21 pairs of SSR primers with clear bands were screened out.90 alleles were amplified,81 of which were polymorphic.The percentage of polymorphic bands was? 90.00%.The number of effective alleles(Ne) ranged from 1.189 2 to 1.994 2.The expected heterozygosity (He) ranged from 0.159 1 to 0.498 5.The change range of the number of Shannon′s information index (I) is between 0.295 8 and 0.691 7.PIC values ranged from 0.146 3 to 0.374 2,and the selected SSR markers showed moderate polymorphism.The results of molecular markers showed that the primers could well reflect the genetic diversity of 18 A.hypochondriacus,which could provide a reference for the development and breeding of A.hypochondriacus resources.
参考文献/References:
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备注/Memo
收稿日期:2021-12-16 基金项目:黑龙江省科研院所科研业务费项目(CZKYF2020C007);黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX08)。 第一作者:张冬梅(1979-),女,硕士,助理研究员,从事牧草栽培、育种技术研究。E-mail:zhd_mei@163.com。 通信作者:王建丽(1977-),女,博士,副研究员,从事牧草分子设计与遗传育种研究。E-mail:wangjianlivip@126.com。