DONG Xiao-jie,LI Zhi-jiang,MA Jin-feng,et al.Sensitivity of Millet Dwarf Materials to Gibberellin During Germination[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(02):18-23.[doi:DOI:10.11942/j.issn1002-2767.2022.02.0018]
谷子矮秆材料萌发期对赤霉素的敏感性分析
- Title:
- Sensitivity of Millet Dwarf Materials to Gibberellin During Germination
- Keywords:
- Setariaitalica L.; germination stage; gibberellin; dwarf breeding
- 文献标志码:
- A
- 摘要:
- 为明确谷子矮秆材料萌发期的赤霉素敏感性反应类型，选用龙谷30×胜谷的杂交组合后代品系JA1~JA10、A1~A10、ZA1~ZA10等30份矮秆材料进行萌发期不同浓度赤霉素处理，分析其根长、中胚轴长、胚芽鞘长、胚芽长以及叶长对GA3的敏感性。结果表明：在两种赤霉素浓度处理条件下，60，80及110 cm株系分离后代各性状对GA3敏感性差异不显著。在GA3敏感性材料中，胚芽的敏感性要强于中胚轴、根、胚芽鞘和叶，且胚芽的敏感性都达到极显著水平以上；其次是中胚轴的敏感性要强于根、胚芽鞘和叶。因此，用GA3鉴定谷子敏感性时，胚芽和中胚轴可以作为鉴定的首选器官。在鉴定GA3敏感性所利用的5个性状中，根长可能会随着赤霉素的处理而减小，说明赤霉素处理可能会对根长产生抑制作用，而其他性状均随着赤霉素处理而增加，即适宜浓度的赤霉素处理对中胚轴长、胚芽鞘长、胚芽长和叶长产生促进作用。本试验利用的30份材料均对GA3处理敏感，表现在不同器官的伸长或抑制，由此推断这些矮秆材料可能含有与GA3合成相关基因，这些材料的矮化可能是由于GA3合成基因发生突变，导致植株不能合成正常生长所需的GA3。
- Abstract:
- In order to clarify the gibberellin sensitivive reaction type of millet dwarf materials at germination stage,30 dwarf materials,which used Longgu 30 as female parent and Shenggu as male parent,dwarf lines JA1-JA10,A1-A10 and ZA1-A10,were treated with gibberellin at different concentrations during germination.The sensitivity of root length,hypocotyl length,coleoptile length,germ length and leaf length to GA3 were analyzed.The results showed that under the treatment of two gibberellin concentrations,there was no significant difference in the sensitivity of the offspring of 60,80 and 110 cm line to GA3.Among the GA3 sensitive materials,the sensitivity of germ was stronger than that of hypocotyl,root,coleoptile and leaf,and the sensitivity of germ reached a very significant level.Secondly,the sensitivity of mesodermal axis was stronger than that of root,coleoptile and leaf.Therefore,when using GA3 to identify millet sensitivity,germ and mesodermal axis can be used as the preferred organs for identification.Among the five traits used to identify GA3 sensitivity,root length may decrease with gibberellin treatment,indicating that gibberellin treatment may inhibit root length,while other traits increase with gibberellin treatment.That is,gibberellin treatment with appropriate concentration canpromote mesodermal axis length,coleoptile length,germ length and leaf length.The 30 materials used in this experiment were sensitive to GA3 treatment,which were manifested in the elongation or inhibition of different organs.It was inferred that these dwarf materials may contain genes related to GA3 synthesis.The dwarfing of these materials may be due to the mutation of GA3 synthesis gene,resulting in the plant unable to synthesize GA3 required for normal plant growth.
参考文献/References:
参考文献： ［1］KONISHI H,YAMANE H,MAESHIMA M,et al.Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling[J].Plant Molecular Biology,2004,56(6):839-848.
［2］KOMATSU S，KONISHI H.Proteome analysis of rice root proteins regulated by gibberellin[J].Genomics Proteomics & Bioinformatics,2005,3(3):132-142.
［3］KOMATSU S,ZANG X,TANAKA N.Comparison of two proteomics techniques used to identify proteins regulated by gibberellin in rice[J].Journal of Proteome Research,2006,5(2):270.
［4］NONOGAKI H. Seed dormancy and germination-emerging mechanisms and new hypotheses［J］.Frontiers in Plant Science,2014,5:233.
［5］邓志军,宋松泉,艾训儒,等.植物种子保存和检测的原理与技术［M］.北京：科学出版社,2019.
［6］徐恒恒,黎妮,刘树君,等.种子萌发及其调控的研究进展［J］.作物学报,2014,40(7):1141-1156.
［7］DENG Z J,CHENG H Y,SONG S Q.Effects of temperature, scarification,dry storage, stratification,phytohormone and light on dormancy-breaking and germination of Cotinus coggygria var.cinerea(Anacardiaceae)seeds［J］.Seed Science & Technology,2010,38(3):572-584.
［8］HIROYUKI N.Seed biology updates-highlights and new discoveries in seed dormancy and germination research［J］.Frontiers in Plant Science,2017,8:524.
［9］李冬玲,段红霞,刘鸿晨,等.赤霉素及其功能类似物与赤霉素受体的研究进展[J].农药学学报,2013,15(6):601-608.
［10］高秀华,傅向东.赤霉素信号转导及其调控植物生长发育的研究进展［J］.生物技术通报,2018,34(7):1-13.
［11］URBANOVA T,LEUBNER-METZGER G.Gibberellin and seed germination［M］//Hedden P,Thomas S G.Annual Plant Reviews,Volume 49:The Gibberellins.Oxford:John Wiley & Sons,Ltd,2016:253-284.
［12］陈金桂,张玉宗.赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定［J］.华北农学报,1998,13(1):46-52.
［13］BEWLEY J D,BRADFORD K J,HILHORST H W M,et al.Seeds:Physiology of development, germination and dormancy,3rd edition［J］.Seed Science Research,2013,23(4):289-289.
［14］OGAWA M,HANADA A,YAMAUCHI Y,et al.Gibberellin biosynthesis and response during Arabidopsis seed germination［J］.Plant Cell,2003,15(7):1591-1604.
［15］CHENG H.Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function［J］.Development, 2004,131(5):1055-1064.
［16］MOSHE J P，MORITZ A.Effects of light,temperature,gibberellin(GA3)and their interaction on coleoptile and leaf elongation of tall,semi-dwarf and dwarf wheat[J].Plant Growth Regulation,1996,18(3):239-247.
［17］钱继岳.谷子矮秆基因的等位性和GA敏感性测定[D].石家庄：河北师范大学,2009.
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备注/Memo
收稿日期：2021-10-17
基金项目：黑龙江省农业科学院2020年度院级科研项目（2020YYYF007）；黑龙江省农业科学院“农业科技创新跨越工程”杂粮杂豆科技创新专项（HNK2019CX05-4）；国家谷子高粱产业技术体系建设专项资金（CARS-06-14.5-B22）；国家重点研发计划（2019YFD1001705-3）。
第一作者：董晓杰（1993-），女，硕士，研究实习员，从事谷子遗传育种研究。E-mail：823807416@qq.com。