DI Gui-li,XU Yan-mei,GAO Chao,et al.Study on Alleviating Salt Stress Injury of Maize Seedlings by Exogenous Ca2+[J].HEILONGJIANG AGRICULTURAL SCIENCES,2022,(02):14-17.[doi:DOI:10.11942/j.issn1002-2767.2022.02.0014]
外源Ca2+缓解玉米幼苗盐胁迫伤害的研究
- Title:
- Study on Alleviating Salt Stress Injury of Maize Seedlings by Exogenous Ca2+
- Keywords:
- exogenous calcium; alleviatory effects; maize; salt stress
- 文献标志码:
- A
- 摘要:
- 为改善大庆盐碱地土壤对农作物生长的抑制,促进耐盐玉米品种的选育,以龙育4号玉米为材料,对玉米幼苗进行NaCl盐胁迫处理,同时添加1,3和5 mmol·L-1的Ca2+缓解盐胁迫,测定玉米幼苗的丙二醛(MDA)、叶绿素含量、过氧化物酶(POD)、可溶性糖含量、可溶性蛋白含量和脯氨酸含量。结果表明:植物受到盐胁迫后与对照相比MDA含量显著增加,叶绿素含量显著降低,POD活性显著增强,渗透调节物质脯氨酸含量和可溶性蛋白增加不显著,但可溶性糖含量显著增加(P<0.05)。加入3 mmol·L-1Ca2+后,与盐胁迫处理相比,MDA、脯氨酸、可溶性蛋白含量及POD活性均降到最低,且与盐胁迫处理差异显著(P<0.05)。综上所述,外源Ca2+对玉米幼苗盐胁迫伤害具有一定的缓解作用,3 mmol·L-1Ca2+处理对玉米幼苗盐胁迫缓解效果最佳。
- Abstract:
- In order to improve the inhibition of saline alkali soil on crop growth in Daqing and promote the breeding of salt tolerant maize varieties,with maize Longyu 4 as the material,maize seedlings were treated with NaCl stress and alleviated by adding (1,3 and 5 mmol·L-1) concentrations of Ca2+,and the contents of malondialdehyde(MDA),chlorophyll,peroxidase (POD),soluble sugar,soluble protein and proline were measured.The results showed that the plant under salt stress compared with the control indicated the MDA content increased,chlorophyll content decreased,POD activity increased and soluble sugar content increased,significant differences(P<0.05).Proline and the content of soluble protein increased,but the differences were not significant.After adding 3 mmol·L-1Ca2+,the contents of MDA,proline,soluble protein and POD activity decreased to the lowest level compared with salt stress group,and the differences were significant(P<0.05).In conclusion,exogenous Ca2+ has a certain alleviating effect on maize seedlings under salt stress,and 3 mmol·L-1Ca2+ treatment has the best alleviating effect on maize seedlings under salt stress.
参考文献/References:
参考文献:
[1]戴高兴,彭克勤,皮灿辉.钙对植物耐盐性的影响[J].中国农学通报,2003,19(3):97-101.
[2]王霞,杨智超,钱海霞,等.添加外源物质硅对NaCl胁迫下玉米幼苗的缓解作用[J].安徽农业科学,2013(17):7404-7405.
[3]李献宇,朱博.大庆市盐碱化土地治理对策初探[J].牡丹江师范学院学报(自然科学版),2006(3):33-35.
[4]李建国,濮励杰,朱明,等.土壤盐渍化研究现状及未来研究热点[J].地理学报,2012,67(9):1233-1245.
[5]胡宝忱,艾军,郭守东,等.盐胁迫对玉米幼苗生长的影响[J].杂粮作物,2008,28(3):166-168.
[6]闫慧萍,彭云玲,赵小强,等.外源24-表油菜素内酯对逆境胁迫下玉米种子萌发和幼苗生长的影响[J].核农学报,2016,30(5):988-996.
[7]卢梦琪,周俊琴,刘懿瑶,等.外源钙离子对NaCl胁迫下油茶花粉萌发的影响[J].中南林业科技大学学报,2021,41(6):91-98.
[8]马超,孔蓓蓓,张均,等.不同引发剂处理对水分胁迫下小麦发芽及幼苗生理特性的影响[J].核农学报,2017,31(2):357-363.
[9]王洪义,王玉凤,杨克军,等.外源NO对NaCl胁迫下玉米幼苗脂质过氧化和抗氧化酶活性的影响[J].玉米科学,2014,22(4):84-87.
[10]魏晓艳,梁丹妮,庞丁铭,等.14个多年生黑麦草品种幼苗期对盐胁迫的生理响应[J].安徽农业科学,2017,45(1):8-12.
[11]谭皓,雷菲.盐胁迫对苏丹草幼苗生长和渗透调节物质含量的影响[J].南方农业,2019,13(28):28-30,34.
备注/Memo
收稿日期:2021-10-25
基金项目:黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX08);黑龙江省科研院所科研业务费(CZYF2020C007)。
第一作者:邸桂俐(1973-),女,硕士,高级农艺师,从事牧草栽培和草地改良。E-mail:diguili59@163.com。
通信作者:边亚娟(1975-),女,博士,副教授,从事微生物研究。E-mail:573637975@qq.com。