[1]赵 洋,李俊杰,苏喆莹,等.白花桔梗不定根固体培养条件的优化研究[J].黑龙江农业科学,2021,(11):69-73.[doi:10.11942/j.issn1002-2767.2021.11.0069]
　ZHAO Yang,LI Jun-jie,SU Zhe-ying,et al.Optimization of Solid Culture Conditions for Adventitious Roots of Platycodon grandiflorum[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(11):69-73.[doi:10.11942/j.issn1002-2767.2021.11.0069]
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白花桔梗不定根固体培养条件的优化研究

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年11 页码: 69-73 栏目: 出版日期: 2021-11-10
Title:
Optimization of Solid Culture Conditions for Adventitious Roots of Platycodon grandiflorum
作者:
赵 洋; 李俊杰; 苏喆莹; 姜 雯; 严一字; 吴松权
延边大学 农学院，吉林 延吉 133002
Author(s):
ZHAO Yang; LI Jun-jie; SU Zhe-ying; JIANG Wen; YAN Yi-zi; WU Song-quan
Agricultural College of Yanbian University,Yanji 133002,China
关键词:
桔梗不定根; 固体培养基; 外源激素; 有效成分
Keywords:
Platycodon grandiflorus adventitious roots; solid medium; exogenous hormones; effective components
DOI:
10.11942/j.issn1002-2767.2021.11.0069
文献标志码:
A
摘要:
为进一步提高白花桔梗的产量，本研究优化了白花桔梗不定根固体培养的条件，包括培养基类型、盐强度、激素浓度和不同生长时期有效成分含量。结果表明：白花桔梗不定根培养基的最优条件为1/2盐浓度B5养基添加0.3 mg·L-1吲哚-3-丁酸（IBA），培养35 d时有效成分含量最高，且皂苷类化合物含量显著高于总黄酮量和多糖量。
Abstract:
In order to further improve the yield of Platycodon grandiflorum,the solid culture conditions of adventitious roots of Platycodon grandiflorum were optimized,including medium type,salt intensity,hormone concentration and effective component content at different growth stages.The results showed that the optimum condition of Platycodon grandiflorum adventitious root culture medium was 1/2 B5 medium supplemented with 0.3 mg·L-1 indole-3-butyric acid(IBA).The content of active components was the highest after 35 days of culture,and the content of saponins was significantly higher than that of total flavonoids and polysaccharides.

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备注/Memo

收稿日期：2021-08-18

基金项目：吉林省科研重点项目（20160204）。
第一作者：赵洋（1996-），女，在读硕士，从事特种植物资源与生物技术研究。E-mail:462479028@qq.com。
通信作者：吴松权（1972-），男，博士，副教授，从事特种植物资源与生物技术研究。E-mail:arswsq@ybu.edu.cn。

更新日期/Last Update: 2021-12-06