[1]周菲.向日葵HaKAR1基因的生物信息学和表达分析[J].黑龙江农业科学,2021,(10):19-22.[doi:DOI:10.11942/j.issn1002-2767.2021.10.0019]
　ZHOU Fei.Bioinformatics and Expression Analysis of HaKAR1Gene in Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(10):19-22.[doi:DOI:10.11942/j.issn1002-2767.2021.10.0019]
点击复制

向日葵HaKAR1基因的生物信息学和表达分析

《黑龙江农业科学》[ISSN:1002-2767/CN:23-1204/S] 卷: 期数: 2021年10 页码: 19-22 栏目: 出版日期: 2021-10-10
Title:
Bioinformatics and Expression Analysis of HaKAR1Gene in Sunflower
作者:
周菲1; 2
1.黑龙江省农业科学院 博士后科研工作站，黑龙江 哈尔滨 150086；2.黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086
Author(s):
ZHOU Fei 1; 2
1.Postdoctoral Programme,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China; 2.Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
关键词:
向日葵; 油酸; HaKAR1; 生物信息学; 表达分析
Keywords:
sunflower;  oleic acid;  HaKAR1;  bioinformatics;  expression anaylsis
DOI:
DOI:10.11942/j.issn1002-2767.2021.10.0019
文献标志码:
A
摘要:
KAR编码β-酮脂酰-ACP还原酶，是在脂肪酸合成过程中催化酮脂酰还原反应的酶。为了解向日葵HaKAR1基因的表达特性及功能，对向日葵HaKAR1编码蛋白进行生物信息学分析和基因表达模式分析，预测HaKAR1编码蛋白为碱性蛋白且为亲水性蛋白，蛋白二级结构主要为α螺旋，预测亚细胞定位于叶绿体。系统进化分析发现，HaKAR1与莴苣（Lactuca sativa L.）KAR1进化关系最近，序列相似性高达90％。基因表达模式分析发现，HaKAR1在向日葵种子、舌状花和管状花中表达量较高，其次为茎、叶，在根中不表达。HaKAR1在高、低油酸向日葵种子开花后7~27 d表达均呈先上升后下降再上升的趋势，但在开花后37 d，低油酸材料种子中表达量明显高于高油酸材料，表明HaKAR1在高、低油酸种子发育后期可能存在不同表达调控机制。
Abstract:
KAR encodes beta-ketoacyl ACP reductase,which catalyzes ketoacyl reduction in fatty acid synthesis.In order to understand the expression characteristics and function of HaKAR gene in sunflower,the bioinformatics of HaKARprotein and gene expression pattern were analyzed.It was predicted that the protein encoded by HaKAR1 was basic protein and hydrophilic protein.The secondary structure of the protein encoded byHaKAR1 was mainly αhelix,and the subcellular localization was predicted in the chloroplast.Phylogenetic analysis showed that HaKAR1 was closely related to Lactuca sativa L.KAR,and the sequence similarity was as high as 90%.The expression pattern analysis of the gene showed that HaKAR1 was highly expressed in sunflower seeds,ligulate flowers and tubular flowers,followed by stems and leaves,but not in roots.The expression ofHaKAR1 in both high and low oleic acid sunflower seeds increased at first and then decreased,and then increased again at 7-27 days after flowering.However,the expression level of HaKAR1 in sunflower seeds with low oleic acid was significantly higher than that in sunflower seeds with high oleic acid at 37 days after flowering,which indicated that there might be different expression regulation mechanisms of HaKAR1 in high and low oleic acid sunflower seeds at late development stage.

参考文献/References:

参考文献：

［1］HUANG J Q,ZHANG T,ZHANG Q X,et al.The mechanism of high contents of oil and oleic acid revealed by transcriptomic and lipidomic analysis during embryogenesis inCarya cathayensis Sarg[J].BMC Genomics,2016,17(1):113.
［2］ZHANG H,JI Y,WANG Y,et al.Cloning and characterization of a novel betaketoacylACP reductase from Comamonas testosteroni[J].Chemicobiological Interactions,2015,234:213220.
［3］ROSADO L A,CACERES R A,JR W F A,et al.Role of Serine140 in the mode of action of Mycobacterium tuberculosis βketoacylACP Reductase (MabA)[J].BMC Research Notes,2012,5(1):526.
［4］FISHER M,KROON J T,MARTINDALE W,et al.The Xray structure of Brassica napusbetaketo acyl carrier protein reductase and its implications for substrate binding and catalysis[J].Structure,2014,8(4):339347.
［5］KLEIN B,PAWLOWSKI K,HRICKEGRANDPIERRE C,et al.Isolation and characterization of a cDNA from Cuphea lanceolataencoding a betaketoacylACP reductase[J].MGGMolecular and General Genetics,1992,233(1-2):122-128.
［6］韩平,阮成江,丁健,等.紫斑牡丹种子高积累碳十八不饱和脂肪酸的多基因调控[J].分子植物育种,2019,17(7):2101-2108.
［7］刘丽,王玉美,赵彦朋,等.棉花脂肪酸合成酶基因GhKAR和GhENR表达载体构建及其功能初探\[J].棉花学报,2016,28(6):527-537.
［8］杨佩.水稻-β酮脂酰ACP还原酶的克隆及功能初探[D].武汉:华中农业大学,2016:16-28.
［9］刘美兰.油桐KAR基因的克隆与功能研究[D].长沙:中南林业科技大学,2015:35-42.
［10］吴波,阮成江,熊朝伟,等.油茶种子油酸合成积累相关基因的表达模式分析[J].分子植物育种,2019,17(5):1493-1502.
［11］张莞晨,阮成江,丁健,等.沙棘果实不同器官脂肪酸差异的多基因协同调控[J].分子植物育种,2019,17(7):2117-2125.
［12］刘祾悦,阮成江,王莉,等.文冠果种仁油脂脂肪酸形成的多基因协同调控[J].分子植物育种,2019,17 (6):1834-1842.

相似文献/References:

[1]刘春来,刘兴龙,王爽,等.不同来源向日葵菌核病分离物致病性分化研究[J].黑龙江农业科学,2013,(04):37.
　LIU Chun-lai,LIU Xing-long,WANG Shuang,et al.Study on Differentiation in Pathogenicity of Sclerotinia sclerotiorum of Sunflowers from Different Regions[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):37.
[2]王文军,李 岑,梁春波,等.钾肥对向日葵产量和品质的调控效应[J].黑龙江农业科学,2012,(12):41.
　WANG Wen-jun,LI Cen,LIANG Chun-bo,et al.Regulating Effect of Potassium on the Yield and Quality of Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2012,(10):41.
[3]王静,黄绪堂,王文军,等.黑龙江省食用向日葵标准化综合高产栽培技术[J].黑龙江农业科学,2013,(08):24.
　WANG Jing,HUANG Xu-tang,WANG Wen-jun,et al.The Standardized Comprehensive High-yield Cultivation Techniques of Edible Sunflower in Heilongjiang Province[J].HEILONGJIANG AGRICULTURAL SCIENCES,2013,(10):24.
[4]马立功,张匀华,孟庆林,等.向日葵黄萎病病原鉴定及其生物学特性研究[J].黑龙江农业科学,2011,(10):40.
　MA Li-gong,ZHANG Yun-hua,MENG Qing-lin,et al.PathogenIdentify and Biological Characteristics of Verticillium wilt of Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2011,(10):40.
[5]崔良基,王德兴,宋殿秀,等.氮磷钾硼配施对向日葵群体生理参数及产量的影响[J].黑龙江农业科学,2010,(09):1.
　CUI Liang-ji,WANG De-xing,SONG Dian-xiu,et al.Effects of Nitrogen，Phosphorus，Potassium and Boron onPhysiological Parameter and Yield of Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):1.
[6]黄绪堂,王文军,张 明,等.黑龙江省向日葵产业存在的问题和发展建议[J].黑龙江农业科学,2010,(09):4.
　HUANG Xu-tang,WANG Wen-jun,ZHANG Ming,et al.Main Problems and Suggestions for Development of Heilongjiang Sunflower Industry[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):4.
[7]孙 敏,蒋文敏,李慧英,等.应用GGE双标图进行向日葵杂交种产量稳定性分析[J].黑龙江农业科学,2010,(09):11.
　SUN Min,JIANG Wen-min,LI Hui-ying,et al.Analysis of Stable-production Character by GGE Biplot on Sunflower Hybrids[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):11.
[8]王德兴,崔良基,孙恩玉,等.密度对不同生育期油葵杂交种产量的影响[J].黑龙江农业科学,2010,(09):28.
　WANG De-xing,CUI Liang-ji,SUN En-yu,et al.Effects of Plant Density on Yield ofDifferent Oilseed Sunflower Hybrids[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):28.
[9]刘 壮,李慧英,于学鹏,等.早熟向日葵品种夏播栽培试验[J].黑龙江农业科学,2010,(09):32.
　LIU Zhuang,LI Hui-ying,YU Xue-peng,et al.The Summer-sowing Cultivation Trial for Early-maturing Sunflower Varieties[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):32.
[10]董 学,张 勤,高立起,等.生根粉双吉尔-GGR-6对向日葵的增产效果[J].黑龙江农业科学,2010,(09):62.
　DONG Xue,ZHANG Qin,GAO Li-qi,et al.Effects of ABT Two-Gill-GGR-6 on the Yield of Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2010,(10):62.
[11]周菲,王文军,刘岩,等.向日葵HafabZ基因的生物信息学和表达分析[J].黑龙江农业科学,2021,(07):11.[doi:10.11942/j.issn1002-2767.2021.07.0011]
　ZHOU Fei,WANG Wen-jun,LIU Yan,et al.Bioinformatics and Expression Analysis of HafabZ Gene in Sunflower[J].HEILONGJIANG AGRICULTURAL SCIENCES,2021,(10):11.[doi:10.11942/j.issn1002-2767.2021.07.0011]

备注/Memo

收稿日期：2021-05-07

基金项目：黑龙江省农业科学院院级科研项目（2020FJZX-005，2019YYYF012）；国家特色油料产业技术体系（CARS-14）；黑龙江省农业科学院“农业科技创新跨越工程”项目（HNK-2019CX06-02，HNK2019CX14）。
作者简介：周菲（1984-），女，博士，助理研究员，从事向日葵品质育种研究。E-mail:zhoufei66666@163.com。

更新日期/Last Update: 2021-11-06